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Plant Growth Regulation

, Volume 67, Issue 1, pp 83–92 | Cite as

Response of antioxidant systems in oxygen deprived suspension cultures of rice (Oryza sativa L.)

  • Revandy Iskandar Damanik
  • Mohd Razi Ismail
  • Zulkifli Shamsuddin
  • Sariam Othman
  • Abd Mohd Zain
  • Mahmood Maziah
Original paper

Abstract

The effect of oxygen deprivation (anoxia) on the antioxidant system in suspension culture of anoxia-intolerant Malaysian rice mutants cells was examined. Abiotic stresses have been reported to adversely affect cell division, damage cellular and organelle membranes. The signaling defense mechanisms, such as molecular and biochemical aspects responding to stress have been proven to be very complex, and still largely untapped. The objective of this study was to determine the potential involvement of activated oxygen species, such as superoxide dismutase, catalase (CAT), ascorbate peroxidase (APX), and glutathione reductase which occur in cells of rice plants exposed to anoxia stress in two Malaysian rice mutants, MR219-4 and MR219-9, and rice cultivar FR13A which is known to be tolerant to anoxia stress during 5–30 days of exposure. The antioxidative enzymes were decreased for MR219-4 and MR219-9 mutants for CAT and APX activities, and increased in FR13A cultivar starting at 20 days in suspension culture compared to that of control. CAT and APX activities were maintained higher in anoxia condition for all mutants and cultivar. These findings suggested that anoxia stress in suspension cultures induced the level of H2O2 to toxic levels.

Keywords

Antioxidant enzymes Suspension culture Cultivars Periods of stress Rice Anoxia stress 

Notes

Acknowledgments

This research was supported by Graduate Research Fund (GRF) of Universiti Putra Malaysia (UPM).

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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Revandy Iskandar Damanik
    • 1
  • Mohd Razi Ismail
    • 2
  • Zulkifli Shamsuddin
    • 3
  • Sariam Othman
    • 4
  • Abd Mohd Zain
    • 5
  • Mahmood Maziah
    • 1
  1. 1.Department of Biochemistry, Faculty of Biotechnology and Biomolecular SciencesUniversiti Putra MalaysiaSerdangMalaysia
  2. 2.Institute of Tropicial AgricultureUniversiti Putra MalaysiaSerdangMalaysia
  3. 3.Department of Land Management, Faculty of AgricultureUniversiti Putra MalaysiaSerdangMalaysia
  4. 4.Rice and Industrial Crop Research Centre MARDI Head QuartersKuala LumpurMalaysia
  5. 5.Department of Agrotechnology, Faculty of Agrotechnology and Food ScienceUniversiti Malaysia TerengganuKuala TerengganuMalaysia

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