Plant Growth Regulation

, Volume 66, Issue 2, pp 203–209 | Cite as

Influence of gibberellin A3 application, pH of the medium, photoperiod and temperature on the enhancement of in vitro flowering in Vitex negundo L.

  • Saikat Gantait
  • Uma Rani Sinniah
  • Perisamy Suranthran
Brief communication


Vitex negundo L. is an economically important small medicinal tree and an essential oil is extracted from its flowers. In nature, flowering is season specific and irregular, appearing only after a long vegetative phase. A very much improved protocol has been developed for the enhancement of flowering in vitro, one which increases both the number of flowers and the percentage (98.6%) of micro-plants flowering. The inclusion of gibberellin A3 (GA3) in the culture medium played the key role in controlling the in vitro flowering in V. negundo. The promotive effect of GA3 was further improved by optimising pH, photoperiod and temperature. Our in vitro flower induction procedures provide an extremely effective method for further research on flowering regulation mechanisms in this important medicinal tree.


Gibberellic acid Micropropagation Murashige and Skoog medium Plant growth regulators Photoperiod Temperature regime 



Gibberellin A3


Murashige and Skoog (1962)


Photosynthetic photon flux density


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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Saikat Gantait
    • 1
  • Uma Rani Sinniah
    • 1
  • Perisamy Suranthran
    • 1
  1. 1.Department of Crop ScienceFaculty of Agriculture, Universiti Putra MalaysiaSerdangMalaysia

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