Influence of gibberellin A3 application, pH of the medium, photoperiod and temperature on the enhancement of in vitro flowering in Vitex negundo L.
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Vitex negundo L. is an economically important small medicinal tree and an essential oil is extracted from its flowers. In nature, flowering is season specific and irregular, appearing only after a long vegetative phase. A very much improved protocol has been developed for the enhancement of flowering in vitro, one which increases both the number of flowers and the percentage (98.6%) of micro-plants flowering. The inclusion of gibberellin A3 (GA3) in the culture medium played the key role in controlling the in vitro flowering in V. negundo. The promotive effect of GA3 was further improved by optimising pH, photoperiod and temperature. Our in vitro flower induction procedures provide an extremely effective method for further research on flowering regulation mechanisms in this important medicinal tree.
KeywordsGibberellic acid Micropropagation Murashige and Skoog medium Plant growth regulators Photoperiod Temperature regime
Murashige and Skoog (1962)
Photosynthetic photon flux density
- Anonymous (2003) The wealth of India, raw material, vol 1 Publication and information directorate. CSIR, New Delhi, pp 522–524Google Scholar
- Chithra M, Martin KP, Sunandakumari C, Madhusoodanan PV (2004) Silver nitrate induced rooting and flowering in vitro on rare rhoeophytic woody medicinal plant, Rotula aquatica Lour. Indian J Biotechnol 3:418–421Google Scholar
- de Jong AW, Bruinsma J (1974) Pistil development in Cleome flowers. III. Effects of growth-regulating substances on flower buds of Cleome iberidella Welw. ex Oliv. grown in vitro. Z Pflanzenphysiol 73:142–151Google Scholar
- Kapur V, Pillai KK, Hussain SZ, Balani DK (1994) Hepatoprotective activity of JIGRINE (An Unani Poly Phasmaontical herbal formulation) on lives damage and lipid peroxidation caused by alcohol-carbon tercichloride and paracetamol in rats. Indian J Pharm Sci 5:160Google Scholar
- Kinet JM, Sachs RM, Bernier G (1985) The Physiology of Flowering. The Development of Flowers, vol III. CRC Press, Boca RatonGoogle Scholar
- Krekule J, Macháčková L, Paplová L, Seidlová F (1989) Hormonal signals in photoperiodic control of flower initiation. In: Krekule J, Seidlová F (eds) Signals in Plant Development. SPB Academic Publishing, The Hague, pp 145–162Google Scholar
- Luckwill LC (1970) The control of growth and fruitfulness of apple trees. In: Luckwill LC, Cutting CV (eds) Physiology of tree crops. Academic Press, London, pp 237–254Google Scholar
- Seidlová F (1989) Signals for changing rates and directions of apical growth operating in flowering. In: Krekule J, Seidlová F (eds) Signals in plant development. SPB Academic Publishiing, The Hague, pp 163–178Google Scholar
- Shu MJ (1994) VITEX Linnaeus, Sp. Pl. 2: 638. 1753. Flora of China 17:28–32Google Scholar
- Vadawale AV, Barve DM, Dave AM (2006) In vitro flowering and rapid propagation of Vitex negundo L.—a medicinal plant. Indian J Biotechnol 5:112–116Google Scholar