Differential expression of farnesyl diphosphate synthase gene from Withania somnifera in different chemotypes and in response to elicitors
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Withania somnifera (L.) Dunal (Family, Solanaceae), commonly known as Ashwagandha is one of the most valuable medicinal plants synthesizing large number of pharmacologically active secondary metabolites known as withanolides. Though the plant has been well characterized in terms of phytochemical profiles as well as pharmaceutical activities, not much is known about the genes responsible for biosynthesis of these compounds. In this study, we have characterized a gene encoding farnesyl diphosphate synthase (FPPS; EC 22.214.171.124), a key enzyme in the pathway of biosynthesis of isoprenoids, from W. somnifera. The full-length cDNA of Withania somnifera FPPS (WsFPPS) of 1,253 bps encodes a polypeptide of 343 amino acids. The amino acid sequence homology and phylogenetic analysis suggest that WsFPPS has close similarity to its counterparts from tomato (SlFPPS) and capsicum (CaaFPPS). Using semi quantitative RT–PCR, the expression pattern of the WsFPPS gene was analyzed in different tissues of Withania chemotypes (NMITLI-101, NMITLI-108, NMITLI-118 and NMITLI-135) as well as in response to elicitors (salicylic acid and methyl jasmonate) and mechanical wounding. The expression analysis suggests that WsFPPS expression varies in different tissues (with maximal expression in flower and young leaf) and chemotypes (with highest level in NMITLI-101) and was significantly elevated in response to salicylic acid, methyl jasmonate and mechanical injury. This is the first report on characterization of an isoprenoid pathway gene involved in withanolide biosynthesis.
KeywordsElicitor response Medicinal plant Mevalonate pathway Withanolides WsFPPS
The authors are thankful to the Council of Scientific and Industrial Research, New Delhi, Govt. of India, for providing the financial support to carry out this work under NMITLI scheme and Network Project (NWP-08).
- Closa M, Vranová E, Bortolotti C, Bigler L, Arró M, Ferrer A, Gruissem W (2010) The Arabidopsis thaliana FPP synthase isozymes have overlapping and specific functions in isoprenoid biosynthesis, and complete loss of FPP synthase activity causes early developmental arrest. Plant J 63:512–525CrossRefGoogle Scholar
- Felsenstein J (1989) PHYLIP—phylogeny inference package (Version 3.2). Cladistics 5:164–166Google Scholar
- Kaileh M, Berghe WV, Boone E, Essawi T, Haegeman G (2007) Screening of indigenous Palestinian medicinal plants for potential anti-inflammatory and cytotoxic activity. J Ethn Pharmacol 113:510–516Google Scholar
- Lal P, Misra L, Sangwan RS, Tuli R (2006) New withanolides from fresh berries of Withania somnifera. Z Naturforsch 61b:1143–1147Google Scholar
- Madina BR, Sharma LK, Chaturvedi P, Sangwan RS, Tuli R (2007) Purification and characterization of a novel glucosyltransferase specific to 27β-hydroxy steroidal lactones from Withania somnifera and its role in stress responses. Biochim Biophys Act 1774:1199–1207Google Scholar
- Misra P, Pandey A, Tiwari M, Chandrashekar K, Sidhu OP, Asif MH, Chakrabarty D, Singh PK, Trivedi PK, Nath P, Tuli R (2010b) Modulation of transcriptome and metabolome of tobacco by Arabidopsis transcription factor, AtMYB12, leads to insect resistance. Plant Physiol 152:2258–2268PubMedCrossRefGoogle Scholar
- Sangwan RS, Chaurasiya ND, Misra LN, Lal P, Uniyal GC, Sharma R, Sangwan NS, Suri KA, Qazi GN, Tuli R (2004) Phytochemical variability in commercial herbal products and preparations of Withania somnifera (Ashwagandha). Curr Sci 86:461–465Google Scholar
- Sanmiya K, Iwasaki T, Matsuoka M, Miyao M, Yamamoto N (1997) Cloning of a cDNA that encodes farnesyl diphosphate synthase and the blue-light-induced expression of the corresponding gene in the leaves of rice plants. Biochim Biophys Act 1350:240–246Google Scholar
- Takaya A, Zhanga YW, Asawatreratanakul K, Wititsuwannakul D, Wititsuwannakul R, Takahashi S, Koyama T (2003) Cloning, expression and characterization of a functional cDNA clone encoding GGPPS of Hevea brassiliensis. Biochem Biophy Act 1625:214–220Google Scholar
- Ven Murthy MR, Ranjekar PK, Ramassamy C, Deshpande M (2010) Scientific basis for the use of Indian Ayurvedic medicinal plants in the treatment of neurodegenerative disorders: Ashwagandha. Cent Nerv Syst Agents Med Chem (in press)Google Scholar