Plant Growth Regulation

, Volume 64, Issue 3, pp 211–219 | Cite as

Chilling-induced changes of vacuolar proton pumps in hypocotyls of Vigna unguiculata

  • Luciana Maia Nogueira de Oliveira
  • Alana Cecília de Menezes Sobreira
  • José Hélio Costa
  • Maria de Lourdes Oliveira Otoch
  • Masayoshi Maeshima
  • Dirce Fernandes de Melo
Original paper


Vigna unguiculata (cowpea) is a legume adapted to high temperatures and is sensitive to low temperatures. Temperature is one of the limiting factors of growth and yield for many crops but its effect on cowpea metabolism is not known. We investigated the effect of chilling on activity of vacuolar proton pumps (V-ATPase and V-PPase) and their protein content in tonoplast vesicles of cowpea hypocotyls. Seedlings grown for 7 days at 10 or 4°C were used for experiments. Chilling treatment at 10 or 4°C markedly suppressed growth of cowpea seedlings. Following chilling at 10 and 4°C, activity of both proton pumps and the relative amount of V-PPase and subunit A of V-ATPase were significantly increased. Both substrate hydrolysis and H+ transport activities of V-PPase remained at relatively high levels during chilling treatment. For V-ATPase, treatment at 10°C for 6 days increased the ATP hydrolysis activity. However, the H+ transport activity of the enzyme was increased when treated for 4 days but was markedly decreased when treated for 6 days. Our results provide evidence for different regulation for these vacuolar proton pumps, indicating that V-PPase is the more stable proton pump throughout chilling stress.


Chilling Cowpea Stress Vacuole V-ATPase V-PPase 











Ethylenediaminetetra-acetic acid


Ethylene glycol-bis (β-aminoethyl ether) N, N, N′, N′-tetraacetic acid


Phenylmethyl-sulfonyl fluoride


Vacuolar H+-ATPase


Vacuolar H+-translocating pyrophosphatase



The CAPES, CNPq and FUNCAP supported this research.


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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Luciana Maia Nogueira de Oliveira
    • 1
    • 2
  • Alana Cecília de Menezes Sobreira
    • 1
    • 3
  • José Hélio Costa
    • 1
  • Maria de Lourdes Oliveira Otoch
    • 3
  • Masayoshi Maeshima
    • 4
  • Dirce Fernandes de Melo
    • 1
  1. 1.Departamento de Bioquímica e Biologia MolecularUniversidade Federal do CearáFortalezaBrazil
  2. 2.Unidade Acadêmica de GaranhunsUniversidade Federal Rural de PernambucoGaranhunsBrazil
  3. 3.Departamento de BiologiaUniversidade Estadual do CearáFortalezaBrazil
  4. 4.Laboratory of Cell Dynamics, Graduate School of Bioagricultural SciencesNagoya UniversityNagoyaJapan

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