Nitrite, sodium nitroprusside, potassium ferricyanide and hydrogen peroxide release dormancy of Amaranthus retroflexus seeds in a nitric oxide-dependent manner
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Nitric oxide (NO) and reactive oxygen species (ROS) are important regulators involving various processes of plant growth and development. Amaranthus retroflexus L. seeds possess a relative dormancy property that means freshly collected seeds can only germinate over a limited, high temperature range. Here, we show that the relative dormancy of A. retroflexus seeds could be significantly released following treatments with exogenous NO/cyanide (CN) donors such as nitrite, gases evolved from acidified nitrite, sodium nitroprusside (SNP), potassium ferricyanide (Fe(III)CN) and gases evolved from SNP or Fe(III)CN solutions, as well as exogenously supplied ROS, hydrogen peroxide (H2O2). However, the effectiveness varied among these chemicals. Gases evolved from acidified nitrite displayed maximum effect while H2O2 had minimum effect. We also show that the effects of these compounds could be significantly inhibited by NO specific scavenger 2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl 3-oxide (PTIO), indicating that NO signaling pathway might play a central role in the dormancy release and germination of A. retroflexus seeds, while both ROS and CN might act through NO-dependent signaling cascades.
KeywordsNitric oxide Nitrite Potassium ferricyanide Reactive oxygen species Seed dormancy Sodium nitroprusside
Coefficient of rate of germination
Reactive oxygen species
We are grateful to Minggao He, Qingyun Wang, Xie Li and Jianqing Ye (Institute of Botany, the Chinese Academy of Sciences) for assisting with germination assays. We also thank Prof. Xiaobai Jin (Institute of Botany, the Chinese Academy of Sciences) for revising the manuscript. This research was financially supported by National Natural Sciences Foundation of China (30870223).
- Bewley JD, Black M (1982) Physiology and biochemistry of seeds in relation to germination, vol. 2. Springer-Verlag, HeidelbergGoogle Scholar
- Bewley JD, Black M (1994) Seed: physiology of development and germination. Plenum Press, New YorkGoogle Scholar
- Fontaine O, Huault C, Pavis N, Billard JP (1994) Dormancy breakage of Hordeum vulgare seeds: effects of hydrogen peroxide and scarification on glutathione level and glutathione reductase activity. Plant Physiol Biochem 32:677–683Google Scholar
- Gallagher RS, Cardina J (1998a) Phytochrome-mediated Amaranthus germination I: effect of seed burial and germination temperature. Weed Sci 46:48–52Google Scholar
- Gallagher RS, Cardina J (1998b) Phytochrome-mediated Amaranthus germination II: development of very low fluence sensitivity. Weed Sci 46:53–58Google Scholar
- Oracz K, El-Maarouf-Bouteau H, Kranner I, Bogatek R, Corbineau F, Bailly C (2009) The mechanisms involved in seed dormancy alleviation by hydrogen cyanide unravel the role of reactive oxygen species as key factors of cellular signaling during germination. Plant Physiol 150:494–505PubMedCrossRefGoogle Scholar
- Schonbeck MW, Egley GH (1980) Redroot pigweed (Amaranthus retroflexus) seed germination responses to afterripening, temperature, ethylene, and some other environmental factors. Weed Sci 28:543–548Google Scholar