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Plant Growth Regulation

, Volume 64, Issue 2, pp 119–128 | Cite as

Isolation and characterization of a harvest-induced promoter of an alfalfa gene, hi7

  • Jian Zhang
  • Wen-Jin Yu
  • Ai-Sheng Xiong
  • Bahman Bahramnejad
  • Larry R. Erickson
Original paper

Abstract

A cDNA library was made by subtractive hybridization of cDNA from non-harvested and harvested alfalfa plants. One cDNA clone, hi7, in Northern blot analysis showed a high level of transcription of this gene in harvested tissue, but not in non-harvested tissue, nor in wounded or heat shock treated tissue. A 640 bp promoter region isolated by genomic walking based on hi7 cDNA sequence, was fused to a GUS gene and transferred to tobacco and Medicago truncatula. When leaf tissue from transgenic tobacco plants was harvested, incubated for 24 h at room temperature, and then stained for GUS activity, a strong blue colour was observed throughout the tissue, but not in tissue that was stained immediately following harvesting. The expression of the GUS gene was also induced in tobacco plants sprayed with abscissic acid (ABA) and in callus derived from these plants when transferred to medium containing ABA.

Keywords

Harvest-induced gene hi7 Promoter ABA Transgenic plants Alfalfa 

Notes

Acknowledgments

Extensive funding for this research was provided by the Natural Sciences and Engineering Research Council of Canada, Strategic Grants Program. As well, we would like to express our appreciation to Dina Hodi for technical supports.

Supplementary material

10725_2010_9546_MOESM1_ESM.doc (1.8 mb)
(DOC 1,839 kb)

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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  1. 1.Plant Agriculture DepartmentUniversity of GuelphGuelphCanada
  2. 2.Biotechnology Research InstituteShanghai Academy of Agricultural SciencesShanghaiChina
  3. 3.Department of Agronomy and Plant BreedingUniversity of KurdistanSanandajIran
  4. 4.Alberta Innovates-Technology Futures/Alberta Research CouncilVegrevilleCanada
  5. 5.Syngenta Biotechnologies, Inc.Research Triangle ParkUSA
  6. 6.Department of Plant AgricultureUniversity of GuelphGuelphCanada

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