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Plant Growth Regulation

, Volume 61, Issue 3, pp 277–286 | Cite as

Genomic analysis of allelopathic response to low nitrogen and barnyardgrass competition in rice (Oryza sativa L.)

  • Chang-Xun Fang
  • Hai-Bin He
  • Qing-Shui Wang
  • Long Qiu
  • Hai-Bin Wang
  • Yue-E Zhuang
  • Jun Xiong
  • Wen-Xiong Lin
Original Research

Abstract

To explore the molecular mechanism of allelopathic rice in response to low nitrogen (N) supply or accompanying weed stress, allelopathic rice PI 312777 and its counterpart Lemont were grown under low N supply or co-cultured with barnyardgrass [Echinochloa crus-galli (L.) Beauv.] in hydroponics. The suppression subtractive hybridization (SSH) technique was employed to isolate the up-regulated genes in the treated rice accession. The results indicated that the expression of the genes associated with N utilization was significantly up-regulated in allelopathic rice PI 312777, and the higher efficiency of N uptake and its utilization were also detected in PI 312777 than that in Lemont when the two rice accessions were exposed to low N supply. This result suggested that the allelopathic rice had higher ability to adapt to low N stress than its non-allelopathic counterpart. However, a different response was observed when the allelopathic rice was exposed to accompanying weed (barnyardgrass) co-cultured in full Hoagland solution (normal N supply). It showed that the expression of the genes associated with allelochemical synthesis and its detoxification were all up-regulated in the allelopathic rice when co-cultured with the target weed under normal N supply. The results suggested that the allelopathic rice should be a better competitor in the rice-weed co-culture system, which could be attributed to increasing de novo biosynthesis and detoxification of allelochemicals in rice, consequently resulting in enhanced allelopathic effect on the target and preventing the autotoxicity in this process. These findings suggested that the accompanying weed, barnyardgrass is not only the stressful factor, but also one of the triggers in activating allelopathy in rice. This implies that the allelopathic rice is sensible of the existing target in chemical communication.

Keywords

Allelopathy Barnyardgrass Gene expression Low nitrogen Plant defense Rice 

Abbreviations

AOC

Allene oxide cyclase

4CL

4-coumarate-CoA ligase

ER

Endoplasmic reticulum

GDH

Glutamate dehydrogenase

GST

Glutathione S-transferase

JA

Jasmonic acid

MT

Metallothionein-like protein

MYB

V-myb avian myeloblastosis viral oncogene homolog

N

Nitrogen

NUE

Nitrogen use efficiency

P450s

Cytochrome P450 monooxygenase

PAL

Phenylalanine ammonia-lyase

SSH

Suppression subtractive hybridization

TRAP

Translocon-associated protein complex

Notes

Acknowledgments

This work was supported by the National Natural Science Foundation of China (No. 30471028) and Provincial Natural Science Foundation of Fujian, China (No. 2009J05045). The authors thank Dr. C.C. Chen for his critical reading of the manuscript.

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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Chang-Xun Fang
    • 1
    • 2
  • Hai-Bin He
    • 1
    • 2
  • Qing-Shui Wang
    • 1
    • 2
  • Long Qiu
    • 1
    • 2
  • Hai-Bin Wang
    • 1
    • 2
  • Yue-E Zhuang
    • 1
    • 2
  • Jun Xiong
    • 1
    • 2
  • Wen-Xiong Lin
    • 1
    • 2
  1. 1.Key Laboratory of Biopesticide and Chemical BiologyMinistry of EducationFuzhouPeople’s Republic of China
  2. 2.Agroecological Institute/School of Life SciencesFujian Agriculture and Forestry University (FAFU)FuzhouPeople’s Republic of China

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