Ultraviolet A-specific induction of anthocyanin biosynthesis and PAL expression in tomato (Solanum lycopersicum L.)
Ultraviolet A (UV-A) is an environmental stimulus, and UV-A-induced anthocyanin biosynthesis has been previously investigated in the tomato plant (Solanum lycopersicum L.). UV-A induced anthocyanin biosynthesis in tomato seedlings and co-irradiation with visible light and UV-A did not influence the content of UV-A-induced anthocyanin accumulation. UV-A irradiation induced significant accumulations of anthocyanin in both the cotyledons and hypocotyls of tomato seedlings. Anthocyanin production increased gradually in the tomato hypocotyls after exposure to UV-A and reached maximum levels at 12 h. In the cotyledons, anthocyanin accumulation was significantly increased at 1 h after UV-A exposure and was reduced afterward; however, it increased again beginning at 3 h. The expression of the phenylalanine ammonia-lyase (SlPAL5) gene was shown to be increased after UV-A exposure in a time-dependent manner. UV-A irradiation was also shown to induce anthocyanin accumulation in the epidermis of the tomato fruit; however, SlPAL5 transcripts were detected only at 3 and 24 h after UV-A treatment. After a 1 h pulse of UV-A, SlPAL5 transcripts were increased significantly in tomato cotyledons and hypocotyls after transfer to dark conditions for a short time.
KeywordsUltraviolet A Phenylalanine ammonia-lyase Solanum lycopersicum L. Anthocyanin
This study was supported by the Research Institute of Bioscience & Biotechnology, Kangwon National University.
- Brődenfeldt R, Mohr H (1988) Time courses for phytochrome-induced enzyme levels in phenylpropanoid metabolism (phenylalanine ammonia-lyase, naringenin-chalcone synthase) compared with time courses for phytochrome-mediated end-product accumulation (anthocyanin, quercetin). Planta 41:383–390CrossRefGoogle Scholar
- Matsumaru K, Kamihama T, Inada K (1971) Covering materials with different transmission properties on anthocyanin content of eggplant pericarp. Environ Control Biol 9:91–97Google Scholar
- McLennan AG (1987) DNA damage, repair, and mutagenesis. In: Bryant JA, Dunham VL (eds) DNA replication in plants. CRC Press, Boca Raton, pp 135–186Google Scholar
- Mol J, Jenkins GI, Schafer E, Weiss D (1996) Signal perception, transduction and gene expression involved in anthocyanin biosynthesis. Crit Rev Plant Sci 15:525–557Google Scholar
- Quail PH (1994) Phytochrome genes and their expression. In: Kendrick RE, Kronenberg GHM (eds) Photomorphogenesis in plants. Kluwer, London, pp 71–104Google Scholar
- Taylor RM, Tobin AK, Bray CM (1997) DNA damage and repair in plants. In: Lumsden PL (ed) Plants and UV-B: responses to environmental change. Cambridge University Press, Cambriage, pp 53–75Google Scholar
- Yarosh DB, Smiles KA (2009) DNA repair and photoprotection. In: Lim HW, Draelos ZD (eds) Clinical guide to sunscreens and photoprotection. Inform Healthcare, New York, pp 169–197Google Scholar