Effect of plant growth regulators on the biosynthesis of vinblastine, vindoline and catharanthine in Catharanthus roseus
Catharanthuse roseus is a well-known medicinal plant for its two valuable anticancer compounds: vinblastine and vincristine, which belongs to terpenoid indole alkaloids. Great efforts have been made to study the principles of its secondary metabolic pathways to regulate the alkaloids biosynthesis. In this article, different plant growth regulators were shortly applied to Catharanthus roseus plants during the blooming period to study their effects on the biosynthesis of vinblastine, vindoline and catharanthine. Salicylic acid and ethylene (ethephon) treatments resulted in a significant increase of vinblastine, vindoline and catharanthine while abscisic acid and gibberellic acid had a strongly negative influence on the accumulation of the three important alkaloids. Methyl jasmonate showed no great effect on the production of these valuable alkaloids. Chlormequat chloride highly enhanced the accumulation of vinblastine but greatly decreased the contents of vindoline and catharanthine.
KeywordsCatharanthus roseus Plant growth regulators Vinblastine Vindoline Catharanthine Blooming period
- C. roseus
Plant growth regulators
Terpenoid indole alkaloids
3′, 4′-anhydrovinblastine synthase
Duncan’s multiple range test
Analysis of variance
This research was supported by China National High-Tech “863” Program (grant number 2007AA10Z189), China “973” Program (grant number 2007CB108805), Shanghai Science and Technology Committee (grant number 08391911800) and Shanghai Leading Academic Discipline Project (Project Number B209).
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