Plant Growth Regulation

, Volume 60, Issue 2, pp 133–141 | Cite as

Effect of plant growth regulators on the biosynthesis of vinblastine, vindoline and catharanthine in Catharanthus roseus

  • Qifang Pan
  • Yu Chen
  • Quan Wang
  • Fang Yuan
  • Shihai Xing
  • Yuesheng Tian
  • Jingya Zhao
  • Xiaofen Sun
  • Kexuan Tang
Original Paper


Catharanthuse roseus is a well-known medicinal plant for its two valuable anticancer compounds: vinblastine and vincristine, which belongs to terpenoid indole alkaloids. Great efforts have been made to study the principles of its secondary metabolic pathways to regulate the alkaloids biosynthesis. In this article, different plant growth regulators were shortly applied to Catharanthus roseus plants during the blooming period to study their effects on the biosynthesis of vinblastine, vindoline and catharanthine. Salicylic acid and ethylene (ethephon) treatments resulted in a significant increase of vinblastine, vindoline and catharanthine while abscisic acid and gibberellic acid had a strongly negative influence on the accumulation of the three important alkaloids. Methyl jasmonate showed no great effect on the production of these valuable alkaloids. Chlormequat chloride highly enhanced the accumulation of vinblastine but greatly decreased the contents of vindoline and catharanthine.


Catharanthus roseus Plant growth regulators Vinblastine Vindoline Catharanthine Blooming period 


C. roseus

Catharanthus roseus


Plant growth regulators


Terpenoid indole alkaloids




Salicylic acid


Gibberellic acid


Abscissic acid


Chlormequat chloride


3′, 4′-anhydrovinblastine synthase


Duncan’s multiple range test


Analysis of variance



This research was supported by China National High-Tech “863” Program (grant number 2007AA10Z189), China “973” Program (grant number 2007CB108805), Shanghai Science and Technology Committee (grant number 08391911800) and Shanghai Leading Academic Discipline Project (Project Number B209).


  1. Abdul Jaleel C, Gopi R, Gomathinayagam M, Panneerselvam R (2009) Traditional and non-traditional plant growth regulators alters phytochemical constituents in Catharanthus roseus. Process Biochem 44:205–209CrossRefGoogle Scholar
  2. Abeles FB, Morgan PW, Saltveit ME Jr (2004) Ethylene in plant biology, 2nd edn. Academic Press, LondonGoogle Scholar
  3. Aerts RJ, Gisi D, De Carolis E, De Luca V, Baumann TW (1994) Methyl jasmonate vapor increases the developmentally controlled synthesis of alkaloids in Catharanthus roseus and Cinchona seedlings. Plant J 5:635–643CrossRefGoogle Scholar
  4. Aerts RJ, Schafer A, Hesse M, Baumann TW, Slusarenko A (1996) Signalling molecules and the synthesis of alkaloids in Catharanthus roseus seedling. Phytochemistry 42:417–422CrossRefGoogle Scholar
  5. Bulgakov VP, Tchernoded GK, Mischenko NP, Khodakovskaya MV, Glazunov VP, Radchenko SV (2002) Effect of salicylic acid, methyl jasmonate, ethephon and cantharidin on anthraquinone production by Rubia cordifolia callus cultures transformed with the rolB and rolC genes. J Biotechnol 97:213–221CrossRefPubMedGoogle Scholar
  6. Decendit A, Liu D, Ouelhazi L, Doireau P, Merillon JM, Rideau M (1992) Cytokini-enhanced accumulation of indole alkaloids in Catharanthus roseus cell cultures: the factors affecting the cytokinin response. Plant Cell Rep 11:400–403CrossRefGoogle Scholar
  7. Dutta A, Singh D, Kumar S, Sen J (2007) Transcript profiling of terpenoid indole alkaloid pathway genes and regulators reveals strong expression of repressors in Catharanthus roseus cell cultures. Plant Cell Rep 26:907–915CrossRefPubMedGoogle Scholar
  8. El-Sayed M, Verpoorte R (2002) Effect of phytohormones on growth and alkaloid accumulation by a Catharanthus roseus cell suspensions cultures fed with alkaloid precursors tryptamine and loganin. Plant Cell Tiss Org Cult 68:265–270CrossRefGoogle Scholar
  9. El-Sayed M, Verpoorte R (2004) Growth, metabolic profiling and enzymes activities of Catharanthus roseus seedlings treated with plant growth regulators. Plant Growth Regul 44:53–58CrossRefGoogle Scholar
  10. El-Sayed M, Verpoorte R (2005) Methyljasmonate accelerates catabolism of monoterpenoid indole alkaloids in Catharanthus roseus during leaf processing. Fitoterapia 76:83–90CrossRefPubMedGoogle Scholar
  11. El-Sayed M, Verpoorte R (2007a) Catharanthus terpenoid indole alkaloids: biosynthesis and regulation. Phytochem Rev 6:277–305CrossRefGoogle Scholar
  12. El-Sayed M, Verpoorte R (2007b) Influence of growth retardants on serpentine accumulation in Cathranthus roseus cell suspensions cultures. Am J Plant Physiol 2:373–377CrossRefGoogle Scholar
  13. Farooqi AHA, Fatima S, Khan A, Sharma S (2005) Ameliorative effect of chloromequat chloride and IAA on drought stressed cultivars of Cymbopogon marani and C. winterianus. Plant Growth Regul 46:277–284CrossRefGoogle Scholar
  14. Godoy-Hernandez G, Loyola-Vargas VM (1997) Effect of acetylsalicylic acid on secondary metabolism of Catharanthus roseus tumor suspensions cultures. Plant Cell Rep 16:287–290CrossRefGoogle Scholar
  15. Haque S, Farooqi AHA, Gupta MM, Sangwan RS, Khan A (2007) Effect of ethrel, chlormequat chloride and paclobutrazol on growth and pyrethrins accumulation in Chrysanthemum cinerariaefolium Vis. Plant Growth Regul 51:263–269CrossRefGoogle Scholar
  16. Hisiger S, Jolicoeur M (2007) Analysis of Catharanthus roseus alkaloids by HPLC. Phytochem Rev 6:207–234CrossRefGoogle Scholar
  17. Lee-Parsons CWT, Ertuk S, Tengtrakool J (2004) Enhancement of ajmalicine production in Catharanthus roseus cell cultures with methyl jasmonate is dependent on timing and dosage of elicitation. Biotechnol Lett 26:1595–1599CrossRefPubMedGoogle Scholar
  18. Naaranlahti T, Auriola S, Lapinjoki SP (1991) Growth-related dimerization of vindoline and catharanthine in Catharanthus roseus and effect of wounding on the process. Phytochemistry 30:1451–1453CrossRefGoogle Scholar
  19. Pasquali G, Goddijn OJM, de Wall A, Verpoorte R, Schilperoort RA, Hoge JHC, Memelink J (1992) Coordinated regulation of two indole alkaloid biosynthetic genes from Catharanthus roseus by auxin and elicitors. Plant Mol Biol 18:1121–1131CrossRefPubMedGoogle Scholar
  20. Roytrakul S, Verpoorte R (2007) Role of vacuolar transporter proteins in plant secondary metabolism: Catharanthus roseus cell culture. Phytochem Rev 6:383–396CrossRefGoogle Scholar
  21. Ruiz-May E, Galaz-Avalos RM, Loyola-Vargas VM (2008) Differential secretion and accumulation of terpene indolie alkaloids in hairy roots of Catharanthus roseus treated with Methyl Jasmonate. Mol Biotechnol 41:278–285CrossRefPubMedGoogle Scholar
  22. Smith JL, Smart NJ, Kurtz WGW, Misawa M (1987) Stimulation of indole alkaloid production in cell-suspensions cultures of Catharanthus roseus by abscisic acid. Planta Med 53:470–474CrossRefPubMedGoogle Scholar
  23. Sottomayor M, López-Serrano M, DiCosmo F, Ros Barceló A (1998) Purification and characterization of alpha-3’, 4’-anhydrovinblastine synthase (peroxidase-like) from Catharanthus roseus (L.) G. Don. FEBS Lett 428:299–303CrossRefPubMedGoogle Scholar
  24. Srivastava NK, Srivastava AK (2007) Influence of gibberellic acid on 14CO2 metabolism, growth, and production of alkaloids in Catharanthus roseus. Photosynthetica 45:156–160CrossRefGoogle Scholar
  25. Verpoorte R, van der Heijden R, Moreno PRH (1997) Biosynthesis of terpenoid indole alkaloids in Catharanthus roseus cells. In: GA Cordell (ed) The alkaloids, vol. 49, pp 221–299Google Scholar
  26. Wingler A, Roitsch T (2008) Metabolic regulation of leaf senescence: interactions of sugar signaling with biotic and abiotic stress responses. Plant Biol Suppl 1:50–62CrossRefGoogle Scholar
  27. Yahia A, Kevers C, Gaspar T, Chénieux JC, Rideau M, Crèche J (1998) Cytokinins and ethylene stimulate indole alkaloid accumulation in cell suspensions cultures of Catharanthus roseus by two distinct mechanisms. Plant Sci 133:9–15CrossRefGoogle Scholar
  28. Zhao J, Verpoorte R (2007) Manipulating indole alkaloid production by Catharanthus roseus cell cultures in bioreactors: from biochemical processing to metabolic engineering. Phytochem Rev 6:435–457CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Qifang Pan
    • 1
  • Yu Chen
    • 1
  • Quan Wang
    • 1
  • Fang Yuan
    • 1
  • Shihai Xing
    • 1
  • Yuesheng Tian
    • 1
  • Jingya Zhao
    • 1
  • Xiaofen Sun
    • 1
  • Kexuan Tang
    • 1
  1. 1.Plant Biotechnology Research Center, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, School of Agriculture and BiologyShanghai Jiao Tong UniversityMinhang District, ShanghaiChina

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