PgWUS expression during somatic embryo development in a Panax ginseng 2c3 cell culture expressing the rolC oncogene
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It is well known that embryogenesis in plants is regulated by the WOX family of transcription factors (W US homeobox); the WUS gene is the founding member of this family. Panax ginseng is one of the most valuable traditional Chinese medicinal herbs, and its reproduction must be maintained to fulfill production demands. A ginseng embryogenic cell culture 2c3 was previously obtained as a result of the transfer of a bacterial oncogene, rolC. This cell line has been used as a model system for studying plant somatic embryogenesis. Several genes that are homologous to the plant WUS genes have been shown to be expressed in the embryogenic 2c3 cell culture; no sequences homologous to WOX genes have been detected. We show that the expression levels of PgWUSII-6, PgWUSII-5, and PgWUSII-8 are significantly higher in the early developmental stages of somatic embryogenesis compared to vector-transformed cells. During late stages of embryogenesis, PgWUSI-3, PgWUSI-7, and PgWUSII-5 expression is activated. We represent the results suggesting that the initiation of P. ginseng somatic embryogenesis may correlate with significant activation of PgWUS expression.
KeywordsPanax ginseng rolC gene Somatic embryogenesis WUS gene
Calcium-dependent protein kinase
This work was supported by a grant from the Russian Foundation for Basic Research, a grant from the President of Russia (MK-714.2008.4), grant from the Far East Division of the Russian Academy of Sciences (06-III-A-06-146), and a grant from the Russian Science Support Foundation.
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