Variations of vinblastine accumulation and redox state affected by exogenous H2O2 in Catharanthus roseus (L.) G. Don
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Effects of exogenous H2O2 application on vinblastine (VBL) and its precursors, vindoline (VIN), catharanthine (CAT) and α-3′,4′-anhydrovinblastine (AVBL), were measured in Catharanthus roseus seedlings in order to explore possible correlation of VBL formation with oxidative stress. VBL accumulation has previously been shown to be regulated by an in vitro H2O2-dependent peroxidase (POD)-like synthase. Experimental exposure of plants to different concentrations of H2O2 showed that endogenous H2O2 and alkaloid concentrations in leaves were positively elevated. The time-course variations of alkaloid concentrations and redox state, reflected by the concentrations of H2O2, ascorbic acid (AA), oxidative product of glutathione (GSSG) and POD activity, were significantly altered due to H2O2 application. The further correlation analysis between alkaloids and redox status indicated that VBL production was tightly correlated with redox status. These results provide a new link between VBL metabolisms and redox state in C. roseus.
KeywordsCatharanthus roseus (L.) G. Don H2O2 Redox state Vinblastine
The authors sincerely thank Dr. Thomas Efferth of German Cancer Research Center for his kind suggestions for this article. This research was financially supported by National Natural Science Foundation of China (30770338) and the Natural Science Foundation of Heilongjiang province (C200511).
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