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Isolation and characterization of the MiCel1 gene from mango: ripening related expression and enhanced endoglucanase activity during softening

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Abstract

Fruit ripening is characterized by the progressive depolymerisation of cell wall polysaccharides of which the cellulose/hemicellulose network forms an important component. We have cloned an endo-β-1,4-glucanase (EGase) homologue, MiCel1 from ripening mango (Mangifera indica var. Dashehari) that shows sequence similarity to higher plant EGase genes. The 2.3 kb cDNA of MiCel1 encodes a putative protein of 619 amino acids with a signal peptide that can direct it to cell walls. It also possesses a cellulose binding domain that is characteristic of microbial endoglucanases. Expression of MiCel1 is fruit specific and ripening related. There is a progressive increase in MiCel1 transcript accumulation during ripening that is correlated with increased EGase activity and associated with decrease in cellulose/hemicellulose content. In control (ethylene untreated) and 1-MCP treated fruit, ripening was delayed by around 3 days. This is associated with a delayed increase in MiCel1 expression and a delayed increase in EGase activity. It is proposed that expression of MiCel1 is closely associated with ripening and may play an important role in mango softening.

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Acknowledgements

We are thankful to Central Institute for Subtropical Horticulture, Lucknow, for the mango samples. Senior Research Fellowship provided to Amita Chourasia by CSIR, India is gratefully acknowledged.

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  1. Plant Gene Expression Lab, National Botanical Research Institute, Lucknow, 226001, India

    Amita Chourasia, Vidhu A. Sane, Rajesh K. Singh & Pravendra Nath

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  1. Amita Chourasia
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  2. Vidhu A. Sane
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  3. Rajesh K. Singh
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  4. Pravendra Nath
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Correspondence to Pravendra Nath.

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Chourasia, A., Sane, V.A., Singh, R.K. et al. Isolation and characterization of the MiCel1 gene from mango: ripening related expression and enhanced endoglucanase activity during softening. Plant Growth Regul 56, 117–127 (2008). https://doi.org/10.1007/s10725-008-9292-5

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