Production of Strigolactones by Arabidopsis thaliana responsible for Orobanche aegyptiaca seed germination
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The germination stimulants produced by Arabidopsis thaliana, a host of root parasitic plants Orobanche spp. but not of arbuscular mycorrhizal (AM) fungi were examined. Root exudates from the hydroponically grown A. thaliana plants were subjected to reverse phase high performance liquid chromatography (HPLC) and retention times of germination stimulants inducing O. aegyptiaca seed germination were compared with those of strigolactone standards. In addition, the root exudates were analyzed by using HPLC linked with tandem mass spectrometry (LC/MS/MS). A. thaliana was found to exude at least three different germination stimulants of which one was identified as orobanchol. This is the first report of strigolactone production by a non-mycotrophic plant. These results together with recent knowledge imply that strigolactones have other unrevealed functions in plant growth and development.
KeywordsArbuscular mycorrhizal fungi Brassicaceae Germination stimulant Orobanchol Root parasitic plants
Days after planting
High-performance liquid chromatography
Liquid chromatography-tandem mass spectrometry
Multiple reaction monitoring
We are grateful to Emeritus Prof. Kenji Mori (The University of Tokyo), Prof. Yukihiro Sugimoto (Kobe University) and Prof. Kohki Akiyama (Osaka Prefecture University) for their generous gifts of strigolactone standards. This study was supported by the JSPS Invitation Fellowship for Research in Japan to Y. G. and by a Grant-in-Aid for Scientific Research (1820810) to K. Y. from Japanese Society for the Promotion of Science (JSPS).
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