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Plant Growth Regulation

, Volume 54, Issue 1, pp 13–21 | Cite as

Stock-plant etiolation causes drifts in total soluble sugars and anthraquinones, and promotes adventitious root formation in teak (Tectona grandis L. f.) coppice shoots

  • Azamal Husen
Original Paper

Abstract

The effects of stock-plant etiolation on coppice-shoot growth, drifts in total soluble sugars and anthraquinones (AQs; C14H8O2), and rooting potentiality of shoot cuttings were examined in Tectona grandis L. f. (clone FG1). When seedlings were one-year-old, they were coppiced and maintained in the dark for etiolation, with a parallel set kept under natural light in an open environment. Coppice shoots were made into single-node leafy cuttings (SNCs), which were cultured under intermittent mist for rooting. These SNCs were treated with different concentrations of NAA (0, 2000 and 3000 mg l−1). Etiolation significantly increased the coppice-shoot length, internode length, number of coppice shoots, number of leaves, number of nodes and total soluble sugars. The HPTLC analysis showed qualitative and quantitative differences in AQs in coppice shoots obtained from etiolated and non-etiolated stock plants. The study showed that AQs could be used as a marker for maturity and juvenility in teak. Stock-plant etiolation caused a significant increase in percent rooting and sprouting, shoot length, number of shoots and number of leaves per SNC, but a decrease in callusing at the base of the SNC. NAA at 2000 and 3000 mg l−1 had inhibitory effects on rooting and sprouting of SNCs. The result showed that stock-plant etiolation fostered rooting by rejuvenating the coppice shoots.

Keywords

Anthraquinones Coppice shoots Cuttings Etiolation NAA Rooting Total sugar Tectona grandis 

Abbreviations

AQ

Anthraquinone

NAA

α-naphthaleneacetic acid

HPTLC

High performance thin layer chromatography

SNC

Single-node leafy coppice-shoot cutting

Notes

Acknowledgments

This work was supported by the World Bank FREE Projects and Indian Council of Forestry Research and Education, Dehra Dun, India. I thank Director and his team (Dr. S. Mahrotra and Dr. Sayyada Khatoon) of National Botanical Research Institute (NBRI), Lucknow, India for providing facilities for HPTLC work.

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Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  1. 1.Plant Physiology, Botany DivisionForest Research InstituteDehra DunIndia
  2. 2.Department of Applied Biology, Faculty of Applied Natural SciencesUniversity of GondarGondarEthiopia

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