Plant Growth Regulation

, Volume 52, Issue 3, pp 249–258 | Cite as

Identification of two new genes from drought tolerant peanut up-regulated in response to drought

  • K. M. Devaiah
  • Geetha Bali
  • T. N. Athmaram
  • M. S. Basha
Original Paper


Using differential display of mRNA transcripts, we obtained nine partial cDNA clones that were up-regulated and down-regulated by exposure to drought. Two full length cDNAs of 825 bp and 700 bp, designated as Arachis hypogaea serine rich protein (AhSrp) and Arachis hypogaea leucine rich protein (AhLrp) were obtained using RLM-RACE. Based on the cDNA sequence, the transcriptional start site of AhSrp and AhLrp was assigned. AhSrp is predicted to code for a protein of 233 amino acids containing a signal peptide, three potential transmembrane domains and a sumolyation motif. AhLrp is predicted to code for a protein of 206 amino acids containing a signal peptide. The expression of partial cDNA and full-length cDNA were confirmed by RNA dot blot analysis. Expression of AhSrp was observed between 6 and 10 days of stress and prominent expression of AhLrp was visualized at 10 days of stress by semi quanititative PCR. Our studies have resulted in the identification of two new genes that may play a role in plant response to drought stress in peanut.


Differential display Drought tolerant variety Peanut Race Semi quantitative PCR 



Arachis hypogaea leucine rich protein


Arachis hypogaea serine rich protein


Complementary DNA


Differential display reverse transcriptase PCR




Drought tolerant variety K-1375


Drought susceptible variety Jl-24


Peanut transcript in drought induced


Peanut transcript drought suppressed


RNA ligase mediated rapid amplification of cDNA ends


Reverse transcriptase PCR



We thank Prof. Gopinathan, Indian Institute of Science, Bangalore, India for providing facilities in his lab for all the DDRT-PCR experiments, Dr. K. S. S. Naik, Senior scientist, Acharya N. G. Ranga Agricultural University (ANGRAU) for providing the K-1375 and Jl-24 peanut varieties and USAID-Peanut CRSP Grant No. FAM # 51 for providing financial support to this work.


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Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • K. M. Devaiah
    • 1
  • Geetha Bali
    • 1
  • T. N. Athmaram
    • 1
  • M. S. Basha
    • 2
  1. 1.Department of Biotechnology, Jnanabharthi CampusBangalore UniversityBangaloreIndia
  2. 2.Plant Biotechnology DivisionFlorida A&M UniversityTallahasseeUSA

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