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Plant Growth Regulation

, Volume 51, Issue 2, pp 159–169 | Cite as

Physiological and biochemical responses of tomato microshoots to induced salinity stress with associated ethylene accumulation

  • Rida A. Shibli
  • Mosbah Kushad
  • Gad G. Yousef
  • Mary Ann Lila
Original Paper

Abstract

Physiological and biochemical responses of open-pollinated ‘Roma’ and dwarf F1 hybrid ‘Patio’ tomato (Lycopersicon esculentum Mill.) cultivars to in vitro induced salinity were examined in light of the possible contribution of ethylene to these symptoms. Salinity was induced by incorporating 0 (control), 50, 100, 150, or 200 mM NaCl into shoot culture media. Elevated salinity treatments significantly enhanced ethylene accumulation in the headspace and were accompanied by increased leaf epinasty in both cultivars. Growth, leaf cell sap osmolarity, leaf tissue viability and shoot soluble protein content were generally depressed with elevated salinity treatments, whereas electrolyte leakage, membrane injury, raffinose, and total sugars were concomitantly increased. Macronutrients N, P, K, Ca, Mg, and S decreased with elevated salinity in both cultivars and were accompanied by a significant increase in Na content and a sharp decrease in K/Na ratio. Tissue micronutrients, Fe, B, Zn, Mn, and Cu were generally decreased with elevated salinity especially at 100 mM or more. Incorporating ethylene inhibitors CoCl2 or NiCl2 at 5.0 or 10.0 mg/l into media supplemented with 100 mM NaCl significantly reduced ethylene accumulation in the headspace and prevented epinasty, but did not eliminate the negative impacts on growth and other physiological parameters caused by salinity treatment in either cultivar. Our results indicate that the increase in ethylene under salinity stress is not the primary factor contributing to salinity’s deleterious effect on tomato plant growth and physiology.

Keywords

Ethylene In vitro Microshoot Salinity Tomato 

Notes

Acknowledgements

Authors would like to thank the Arab Fund Fellowship Program, Arab Fund for Social Development, Kuwait, Jordan University of Science and Technology, Jordan, the Office of Research, College of Agriculture, Consumer and Environmental Sciences, and the International Council at University of Illinois, for their support during the course of this study. This project was conducted under the terms of a Memorandum of Understanding between University of Illinois and Jordan University of Science and Technology which allowed author Shibli to spend his sabbatical at University of Illinois during the course of this study.

References

  1. Abed Alrahman NM, Shibli RA, Ereifej KI, Hindiyeh MY (2005) Influence of salinity on growth and physiology of in vitro grown cucumber (Cucumis sativus L.). Jordan J Agric Sci 1:93–106Google Scholar
  2. Al-Karaki GN (2000) Growth, sodium and potassium uptake and translocation in salt stressed tomato. J Plant Nutr 23:369–379Google Scholar
  3. Arbona V, Marco AJ, Iglesias DJ, Lopez-Climent MF, Talon M, Gomez-Cadenas A (2005) Carbohydrate depletion in roots and leaves of salt-stressed potted Citrus clementina L. Plant Growth Regul 46:153–160CrossRefGoogle Scholar
  4. Bais HP, Sudha GS, Ravishankar GA (2000) Putrescine and silver nitrate influences shoot multiplication, in vitro flowering and endogenous titers of polyamines in Chicorium intybus L. CV. Lucknow local. J Plant Growth Regul 19:238–248PubMedGoogle Scholar
  5. Bradford MM (1976) A rapid and sensitive method for the quntitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254PubMedCrossRefGoogle Scholar
  6. Cano EA, Perez-Alfocea F, Moreno V, Caro M, Bolarin MC (1998) Evaluation of salt tolerance in cultivated and wild tomato species through in vitro shoot apex culture. Plant Cell Tissue Org Cult 53:19–26CrossRefGoogle Scholar
  7. Cramer GR, Lauchli A, Polito A (1985) Displacement of Ca by Na from the plasma lemma of root cells. A primary response to salt stress. Plant Physiol 79:207–211PubMedGoogle Scholar
  8. Croughan TP, Stavarek SJ, Rains DW (1981) In vitro development of salt resistant plants. Envion Exp Bot 21:317–324CrossRefGoogle Scholar
  9. Essa TA (2002) Effect of salinity stress on growth and nutrient composition of three soybean (Glycine max L. Meerrill) cultivars. J Agron Crop Sci 188:86–93CrossRefGoogle Scholar
  10. Flores P, Carvajal M, Cerda A, Martinez V (2001) Salinity and ammonium/nitrate interactions on tomato plant development, nutrition, and metabolites. J Plant Nutr 24:1561–1573CrossRefGoogle Scholar
  11. Gunes A, Inal A, Alpaslan M (1996) Effect of salinity on stomatal resistance, proline, and mineral composition of pepper. J Plant Nutr 19:389–396Google Scholar
  12. Hopkins WG (1995) Introduction to plant physiology. Wiley, New York, pp 72–73Google Scholar
  13. Jones RA, El-Abd SO (1989) Prevention of salt-induced epinasty by α-aminooxy acid and cobalt. Plant Growth Regul 8:315–323CrossRefGoogle Scholar
  14. Kaya C, Kirnak H, Higgs D (2001) Enhancement of growth and normal growth parameters by foliar application of potassium and phosphorous in tomato cultivars grown at high (NaCl) salinity. J Plant Nutr 24:357–367CrossRefGoogle Scholar
  15. Kim CY, Liu Y, Thorne ET, Yang H, Fukushige H, Grassman W, Hilderbrand D, Sharp RE, Zhang S (2003) Activation of stress-responsive mutagen-activated protein kinase cascade induces the biosynthesis of ethylene in plants. Plant Cell 15:2707–2718PubMedCrossRefGoogle Scholar
  16. Knight SL, Rogers RB, Smith MAL, Spomer LA (1992) Effect of NaCl salinity on miniature dwarf tomato ‘Micro-Tom’. І. Growth analysis and nutrient composition. J Plant Nutr 15:2351–2327Google Scholar
  17. Lutts S, Kinet J-M, Bouharmont J (1996) Ethylene production by leaves of rice (Oryza sativa L.) in relation to salinity tolerance and exogenous putrescine application. Plant Sci 16:15–25CrossRefGoogle Scholar
  18. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497CrossRefGoogle Scholar
  19. Naeini MR, Koshgofratmanesh AH, Lessani H, Falahi E (2004) Effects of sodium-chloride salinity on mineral nutrient and soluble sugars in three commercial cultivars of pomegranate. J Plant Nutr 27:1319–1326CrossRefGoogle Scholar
  20. Pardossi A, Malorgio F, Tognoni F (1999) Salt tolerance and minerals relations for celery. J Plant Nutr 22:151–161Google Scholar
  21. Perez-Alfocea F, Estan MT, Caro M, Bolarin MC (1993) Responses of tomato cultivars to salinity. Plant Soil 150:203–211CrossRefGoogle Scholar
  22. Rus AM, Panoff M, Perez-Alfocea F, Bolarin M (1999) NaCl Reponses in tomato calli and whole plant. J Plant Physiol 155:727–733Google Scholar
  23. Saleh-Lakha S, Grichko VP, Sisler EC, Glick BR (2005) The effect of ethylene action inhibitor 1-cyclopropenylmethyl butyl ether on early plant growth. J Plant Growth Regul 23:307–312CrossRefGoogle Scholar
  24. Satti SME, Lopez M (1994) Effect of increasing potassium levels for alleviating sodium chloride stress on the growth and yield of tomato. Commun Soil Sci Plant Anal 25:2807–2823Google Scholar
  25. Shibli RA, Sawwan J, Swaidat I, Tahat M (2001) Increased phosphorus mitigates the adverse effects of salinity in tissue culture. Communic Soil Sci Plant Anal 32:429–440CrossRefGoogle Scholar
  26. Shibli RA, Smith MAL, Kushad M (1997a) Headspace ethylene accumulation effects on secondary metabolite production in Vaccinium pahalae cell culture. Plant Growth Regul 23:201–205CrossRefGoogle Scholar
  27. Shibli RA, Smith MAL, Nasr R (1997b) Iron source and cytokinin mitigate the incidence of chlorosis and hyperhydration in vitro. J Plant Nutr 20:773–781CrossRefGoogle Scholar
  28. Stavarek SJ, Rains DW (1983) Mechanisms for salinity tolerance in plants. Iowa State J Res 57:457–476Google Scholar
  29. Sudhakar C, Lakshmi A, Giridarakumar S (2001) Changes in the antioxidant enzyme efficacy in two high yielding genotypes of mulberry (Morus alba L.). Plant Sci 161:613–619CrossRefGoogle Scholar
  30. Watson NE, Galliher TL (2001) Comparison of Dumas and Kjeldhal methods with automatic analyzers on agricultural samples under routine rapid analysis conditions. J Plant Nutr 32:2007–2019Google Scholar

Copyright information

© Springer Science+Business Media B.V. 2006

Authors and Affiliations

  • Rida A. Shibli
    • 1
  • Mosbah Kushad
    • 1
  • Gad G. Yousef
    • 1
  • Mary Ann Lila
    • 1
  1. 1.Department of Natural Resources and Environmental SciencesUniversity of IllinoisUrbanaUSA

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