Morphactin influences guggulsterone production in callus cultures of Commiphora wightii
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Guggulsterone, a hypolipidemic natural agent, is produced in resin canals of the plant Commiphora wightii. In this study, the efficacy of different plant growth regulators was evaluated for optimizing its production. Morphactin was found to be effective in enhancing the accumulation of guggulsterones in callus cultures. Maximum callus growth was recorded on medium containing morphactin (0.1 mg l−1) and 2iP (2.5 mg l−1), whereas maximum guggulsterone production occurred when the calluses were cultured on medium containing 0.1 mg l−1 morphactin and 1.0 mg l−1 2iP. Morphactin and 2iP interacted significantly to enhance the callus growth and guggulsterone production by about 8-folds in one-year-old cultures. However, the effect of morphactin on callus growth and guggulsterone production was not uniform over the levels of 2iP tested. Such an effect of morphactin has never been reported on the production of secondary metabolites.
KeywordsCallus culture Commiphora wightii Guggulsterone Morphactin Plant growth regulators Secondary metabolites
N 6-(2-Isopentenyl) adenine
Murashige and Skoog’s (1962) medium
This work was supported by financial assistance (grant no. BT/PR3214/PBD/17/210/2002) from the DBT, Government of India, New Delhi and partially by DST-FIST programme for infrastructure development and UGC-DRS under special assistance programme for medicinal plant research to KGR. We thank Dr. B. R. Ranwa, Rajasthan College of Agriculture, Udaipur for statistical analysis.
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