Plant Growth Regulation

, Volume 51, Issue 1, pp 93–98 | Cite as

Morphactin influences guggulsterone production in callus cultures of Commiphora wightii

  • Y. S. Tanwar
  • Meeta Mathur
  • K. G. Ramawat
Original Paper


Guggulsterone, a hypolipidemic natural agent, is produced in resin canals of the plant Commiphora wightii. In this study, the efficacy of different plant growth regulators was evaluated for optimizing its production. Morphactin was found to be effective in enhancing the accumulation of guggulsterones in callus cultures. Maximum callus growth was recorded on medium containing morphactin (0.1 mg l−1) and 2iP (2.5 mg l−1), whereas maximum guggulsterone production occurred when the calluses were cultured on medium containing 0.1 mg l−1 morphactin and 1.0 mg l−1 2iP. Morphactin and 2iP interacted significantly to enhance the callus growth and guggulsterone production by about 8-folds in one-year-old cultures. However, the effect of morphactin on callus growth and guggulsterone production was not uniform over the levels of 2iP tested. Such an effect of morphactin has never been reported on the production of secondary metabolites.


Callus culture Commiphora wightii Guggulsterone Morphactin Plant growth regulators Secondary metabolites 



N 6-(2-Isopentenyl) adenine


2,4,5-trichlorophenoxyacetic acid


Dry mass


Fresh mass




Murashige and Skoog’s (1962) medium



This work was supported by financial assistance (grant no. BT/PR3214/PBD/17/210/2002) from the DBT, Government of India, New Delhi and partially by DST-FIST programme for infrastructure development and UGC-DRS under special assistance programme for medicinal plant research to KGR. We thank Dr. B. R. Ranwa, Rajasthan College of Agriculture, Udaipur for statistical analysis.


  1. Barve DM, Mehta AR (1993) Clonal propagation of mature elite trees of Commiphora wightii. Plant Cell Tiss Org Cult 35:237–244CrossRefGoogle Scholar
  2. Dev S (1999) Ancient-modern concordance in Ayurvedic plants: some examples. Environ Health Perspect 10:783–789CrossRefGoogle Scholar
  3. Kumar S, Shankar V (1982) Medicinal plants of the Indian desert: Commiphora wightii (Arnott) Bhandari. J Arid Environ 5:1–11Google Scholar
  4. Kumar S, Mathur M, Jain AK, Ramawat KG (2006) Somatic embryo proliferation in Commiphora wightii and evidence for guggulsterone production in culture. Indian J Biotechnol 5:217–222Google Scholar
  5. Kumar S, Suri SS, Sonie KC, Ramawat KG (2003) Establishment of embryonic cultures and somatic embryogenesis in callus culture of guggul-Commiphora wightii (Arnott.) Bhandari. Indian J Exp Biol 4:69–77Google Scholar
  6. Kumar S, Sonie KC, Ramawat KG (2004) Development of resin canals during somatic embryogenesis in callus cultures of Commiphora wightii. Indian J Biotechnol 3:267–270Google Scholar
  7. Mathur M, Jain AK, Ramawat KG (2005) Estimation of guggulsterones in Commiphora wightii in vivo and in vitro using HPLC. In: Proceedings of the national symposium on botanical products in new millennium: developments and challenges, University of Rajasthan, Jaipur, 5–7 February 2005Google Scholar
  8. Mesrob B, Nesbitt C, Misra R, Pandey RC (1998) High-performance liquid chromatographic method of fingerprinting and quantitative determination of E- and Z-guggulsterones in Commiphora mukul resin and its products. J Chromatography B 720:189–196CrossRefGoogle Scholar
  9. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 1:473–497CrossRefGoogle Scholar
  10. Nema RK (2004) Biotechnological and phytochemical approaches to the medicinal herbs of Aravalli hills. PhD Dissertation, ML Sukhadia UniversityGoogle Scholar
  11. Owsley E, Chiang JYL (2003) Guggulsterone antagonizes farnesoid X receptor induction of bile salt export pump but activates pregnane X receptor to inhibit cholesterol 7α-hydroxylase gene. Biochem Biophys Res Comm 30:191–195CrossRefGoogle Scholar
  12. Ramawat KG, Mathur M (2006) Factors affecting production of secondary metabolites. In: Ramawat KG, Merillon JM (eds) Biotechnology: secondary metabolites. Science Publishers, Inc., Enfield, USA, pp 59–102Google Scholar
  13. Ramawat KG, Arya HC (1977) Growth and morphogenesis in callus cultures of Ephedra gerardiana. Phytomorphology 26:395–403Google Scholar
  14. Singh AK, Suri SS, Ramawat KG (1997) Somatic embryogenesis from immature zygotic embryos of Commiphora wightii, a woody medicinal plant. Gartenbauwissenschaft 6:44–48Google Scholar
  15. Suri SS, Ramawat KG (1995) In vitro hormonal regulation of laticifers differentiation in Calotropis procera. Ann Bot 75:477–480CrossRefGoogle Scholar
  16. Urizar NL, Liverman AB, Dodds DT, Silva FV, Ordentlich P, Yan Y, Gonzalez FJ, Heyman RA, Mangelsdorf DF, Moore DD (2002) A natural product that lowers cholesterol as an antagonist ligand for FXR. Science 26:1703–1706CrossRefGoogle Scholar
  17. Wang X, Greilberger J, Ledinski G, Kager G, Paigen B, Jurgens G (2004) The hypolipidemic natural product Commiphora mukul and its component guggulsterone inhibit oxidative modification of LDL. Atherosclerosis 172:239–246PubMedCrossRefGoogle Scholar
  18. Wu J, Xia C, Meier J, Li S, Hu X, Lala DS (2002) The hypolipidemic natural product guggulsterone acts as an antagonist of bile acid receptor. Mol Endocrinol 1:1590–1597CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2006

Authors and Affiliations

  1. 1.Laboratory of Bio-Molecular Technology, Department of BotanyM. L. Sukhadia UniversityUdaipurIndia

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