Plant Growth Regulation

, Volume 51, Issue 1, pp 21–31 | Cite as

Changes in concentrations of soluble carbohydrates during germination of Amaranthus caudatus L. seeds in relation to ethylene, gibberellin A3 and methyl jasmonate

  • B. Białecka
  • J. Kępczyński
Original Paper


Unimbibed Amaranthus caudatus seeds were found to contain stachyose, raffinose, verbascose, sucrose, galactinol, myo-inositol, glucose and fructose, while no galactose, maltose and maltotriose was detected. During imbibition, seed concentrations of verbascose, stachyose, raffinose, galactinol, myo-inositol (temporary) and fructose (transient) were observed to decrease; concentrations of galactose and maltose remained fairly constant, while those of sucrose, glucose and maltotriose increased, the increase in sucrose concentration was only temporary. Effects of gibberellin A3 (GA3) at 3 × 10−4 M and ethephon at 3 × 10−4 M alone or in the presence of methyl jasmonate (Me-JA) at 10−3 M on concentrations of soluble sugars during germination of A. caudatus seeds were examined. Me-JA was found to inhibit seed germination and fresh weight of the seeds, but did not affect sucrose, myo-inositol, galactose and maltose concentrations during imbibition for up to 20 h. The exogenously applied GA3 was observed to enhance germination, stachyose breakdown and glucose concentration after 20 h of incubation. Ethephon stimulated seed germination as well as utilisation of stachyose, galactinol (both after 14 and 20 h) and raffinose (after 14 h of incubation). Although the stimulatory effect of either GA3 or ethephon on seed germination was blocked by Me-JA; these stimulators increased mobilisation of raffinose and stachyose, but only ethephon enhanced both glucose and fructose after 14 and/or 20 h of incubation in the presence of Me-JA. The maltose concentration was increased by both GA3 and ethephon alone and in the presence of Me-JA. Of the growth regulators studied, ethephon alone and/or in combination with Me-JA significantly increased the concentrations of glucose, fructose, galactose, maltose and maltotriose. The differences in sugar metabolism appear to be linked to ethylene or GA3 applied simultaneously with Me-JA.


Amaranthus caudatus seeds Ethylene GA3 Germination Methyl jasmonate Soluble sugars 



We wish to thank you Dr. E. Demole, Firmenich S.A. Geneva, Switzerland for the gift of methyl jasmonate. We are very grateful Editor in Chief C.J. Atkinson for correcting of English grammar.


  1. Bentsink L, Alonso-Blanco C, Vreugdenhil D, Tesnier K, Groot SPC, Koornneef M (2000) Genetic analysis of seed-soluble oligosaccharides in relation to seed storability of Arabidopsis. Plant Physiol 124:1595–1604PubMedCrossRefGoogle Scholar
  2. Bewley JD, Black M (1994) Seeds. Physiology of development and germination, 2nd edn. Plenum Press, New York/LondonGoogle Scholar
  3. Białecka B, Kępczyński J (2003) Regulation of α-amylase activity in Amaranthus caudatus seeds by methyl jasmonate, gibberellin A3, benzyladenine and ethylene. Plant Growth Regul 39:51–56CrossRefGoogle Scholar
  4. Bogatek R, Come D, Corbineau F, Ranjan R, Lewak S (2002) Jasmonic acid affects dormancy and sugar catabolism in germinating apple embryos. Plant Physiol Biochem 40:167–173CrossRefGoogle Scholar
  5. Brillouet JM, Riochet D (1983) Cell wall polysaccharides and lignin in cotyledons and hulls of seeds from various lupin (Lupinus L.) species. J Sci Food Agric 34:861–868CrossRefGoogle Scholar
  6. Buckeridge MS, Dietrich SMC (1996) Mobilisation of the raffinose family oligosaccharides and galactomannan in germinating seeds of Sesbania marginata Benth. (Leguminosae-Faboideae). Plant Sci 117:33–43CrossRefGoogle Scholar
  7. Buckeridge MS, Pessoa dos Santos H, Tine MAS (2000) Mobilisation of storage cell wall polysaccharides in seeds. Plant Physiol Biochem 38:141–156CrossRefGoogle Scholar
  8. Cerning-Beroard J, Filiarte A (1976) A comparison of the carbohydrate composition of legume seeds: horse, beans, peas and lupines. Cereal Chem 53:968–978Google Scholar
  9. Colmenares de Ruiz AS, Bressani R (1990) Effect of germination on the chemical composition and nutritive value of amaranth grain. Cereal Chem 67:519–522Google Scholar
  10. Dirk LMA, van der Krol AR, Vreugdenhil D, Hilhorst HWM, Bewley JD (1999) Galactomannan, soluble sugar and starch mobilization following germination of Trigonella foenum-graecum seeds. Plant Physiol Biochem 37:41–50CrossRefGoogle Scholar
  11. Drewes FE, Van Staden J (1991) Reserve mobilization during germination of Tagetes minuta L. Ann Bot 68:79–83Google Scholar
  12. Górecki RJ, Piotrowicz-Cieślak AI, Lahuta LB, Obendorf RL (1997) Soluble carbohydrates in desiccation tolerance of yellow lupin seeds during maturation and germination. Seed Sci Res 7:107–115Google Scholar
  13. Gurusinghe S, Bradford KJ (2001) Galactosyl-sucrose oligosaccharides and potential longevity of primed seeds. Seed Sci Res 11:121–133Google Scholar
  14. Hill LM, Morley-Smith ER, Rawsthorne S (2003) Metabolism of sugars in the endosperm of developing seeds of oilseed rape. Plant Physiol 131:228–236PubMedCrossRefGoogle Scholar
  15. Hitz WD, Carlson TJ, Kerr PS, Sebastian SA (2002) Biochemical and molecular characterization of a mutation that confers a decreased raffinosaccharide and phytic acid phenotype on soybean seeds. Plant Physiol 128:650–660PubMedCrossRefGoogle Scholar
  16. Horbowicz M, Obendorf RL (1994) Seed desiccation tolerance and storability: dependence on flatulence-producing oligosaccharides and cyclitols-review and survey. Seed Sci Res 4:385–405Google Scholar
  17. Kamalavalli D, Prathapasenan G, Rao R, Pathak CH (1972) Metabolic changes during germination of cotton and sorghum and the role of gibberellic acid. Biochem J 128:55Google Scholar
  18. Kaur S, Gupta AK, Kaur N (1998) Gibberellin A3 reverses the effect of salt stress in chickpea (Cicer arietinum L.) seedlings by enhancing amylase activity and mobilization of starch in cotyledons. Plant Growth Regul 26:85–90CrossRefGoogle Scholar
  19. Kaur S, Gupta AK, Kaur N (2000) Effect of GA3, kinetin and indole acetic acid on carbohydrate metabolism in chickpea seedlings germinating under water stress. Plant Growth Regul 30:61–70CrossRefGoogle Scholar
  20. Kępczyński J, Białecka B (1994) Stimulatory effect of ethephon, ACC, gibberellin A3 and A4+7 on germination of methyl jasmonate inhibited Amaranthus caudatus L. seeds. Plant Growth Regul 14:211–216CrossRefGoogle Scholar
  21. Kępczyński J, Białecka B, Kępczyńska E (1999) Ethylene biosynthesis in Amaranthus caudatus seeds in response to methyl jasmonate. Plant Growth Regul 28:59–65CrossRefGoogle Scholar
  22. Kontos F, Spyropulos CG (1996) Effect of linoleic, linolenic and jasmonic acid on the production of α-galactosidase and endo-β-mannanase activity in the endosperms of carob and fenugreek seeds. J Plant Physiol 149:629–632Google Scholar
  23. Lahuta LB, Górecki RJ, Michalczyk D, Piotrowicz-Cieślak AI (2000) Apha-d-galactosidase activity in stored yellow lupin (Lupinus luteus L.) seeds. Electronic J Polish Agric. Univ. Available online:
  24. Loewus FA, Murthy PPN (2000) myo-Inositol metabolism in plants. Plant Sci 150:1–19CrossRefGoogle Scholar
  25. Obendorf RL (1997) Oligosaccharides and galactosyl cyclitols in seed desication tolerance. Seed Sci Res 7:63–74CrossRefGoogle Scholar
  26. Peterbauer T, Richter A (2001) Biochemistry and physiology of raffinose family oligosaccharides and galactosyl cyclitols in seeds. Seed Sci Res 11:185–197Google Scholar
  27. Spyropoulos CG, Reid JSG (1985) Regulation of α-galactosidase activity and the hydrolases of galactomannan in the endosperm of the fenugreek (Trigonella foenu-graecum) seeds. Planta 166:271–275CrossRefGoogle Scholar
  28. Thind SK, Malik CP (1995) Carbohydrate metabolism in okra (Abelmoschus esculentus L.). Acta Physiol Plant 17:321–326Google Scholar

Copyright information

© Springer Science+Business Media B.V. 2006

Authors and Affiliations

  1. 1.Department of Plant Physiology and BiotechnologyUniversity of SzczecinSzczecinPoland

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