Plant Growth Regulation

, Volume 51, Issue 1, pp 33–39 | Cite as

Repetitive somatic embryogenesis in carnation on picloram supplemented media

Original Paper


An efficient method of repetitive somatic embryogenesis and plant regeneration of two carnation cultivars (Sagres and Impulse) was established using a two-step protocol. In the first step, embryogenic tissue were induced from petal explants on MS culture medium containing 9% sucrose (w/v), 9 μM 2,4-D and 0.8 μM BA. In the second step, embryogenic tissue transferred onto the MS medium containing 3% sucrose supplemented with different concentrations of picloram (0.8, 2, 4, 8 and 16 μM) to produce primary somatic embryos. Precotyledonary clumps and cotyledonary somatic embryos were then isolated and subcultured onto the same media as the second step where they formed secondary somatic embryos in repetitive cycles. Cotyledony somatic embryos were converted into plantlets when they transferred onto the growth regulator-free half-strength MS medium followed by being acclimated to the greenhouse conditions.


Somatic embryogenesis Embryogenic tissue Carnation 





2,4-Dichlorophenoxyacetic acid


Murashige and Skoog (1962) basal medium


4-amino-3,5,6-trichloro-2-pyridinecarboxylic acid


α-naphthalene acetic acid


Indole-3-butyric acid


Indole acetic acid


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Copyright information

© Springer Science+Business Media B.V. 2006

Authors and Affiliations

  1. 1.Department of Biotechnology, Faculty of AgricultureBu-Ali Sina UniversityHamadanIran
  2. 2.Department of Horticulture, Faculty of AgricultureBu-Ali Sina UniversityHamadanIran

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