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Plant Growth Regulation

, Volume 51, Issue 1, pp 11–19 | Cite as

The dynamics of nutrient utilization and growth of apple root stock ‘M9 EMLA’ in temporary versus continuous immersion bioreactors

  • D. Chakrabarty
  • Y. H. Dewir
  • E. J. Hahn
  • S. K. Datta
  • K. Y. Paek
Original Paper

Abstract

The present study investigated the dynamics of nutrient utilization and various growth and physiological parameters during in vitro proliferation of apple root stock ‘M9 EMLA’ in two different bioreactor systems, i.e. temporary and continuous immersions. Individual shoots obtained from temporary immersion system had higher dry mass and were of better quality than those obtained from continuous immersion. In continuous immersion bioreactor, apple shoots appeared to utilize more nutrients from liquid culture medium than that from temporary immersion. The shoot growth was limited by the availability of phosphate and nitrogen in continuous immersion system. The shoots produced in temporary immersion bioreactor showed higher photosynthetic rate, maximum quantum yield of photosystem-II and slow but steady rate of nutrient absorption, indicating the occurrence of higher photomixotrophic metabolism. The study also showed that high level of antioxidant scavenging enzymes in shoots grown in continuous immersion system induced physiological changes to foster adaptation to stresses.

Keywords

Antioxidant enzymes Apple Bioreactor Continuous immersion system Fv/Fm Nutrient utilization Temporary immersion system 

Abbreviations

APX

Ascorbate peroxidase

BTBB

Balloon-type bubble bioreactor

BA

N6-benzyladenine

CAT

Catalase

IBA

Indole-3-butyric acid

G-POD

Guaiacol peroxidase

GA3

Gibberellic acid

GR

Glutathione reductase

MS

Murashige and Skoog’s (1962) medium

PPFD

Photosynthetic photon flux density

ROS

Reactive oxygen species

SOD

Superoxide dismutase

Notes

Acknowledgements

This work was financially supported by the Ministry of education and Human Resource Development (MOE), the Ministry of Commerce, Industry and Energy (MOCIE) and the Ministry of Labor (MOLAB) through the fostering project of the Lab of Excellency. DC acknowledges the KOSEF for providing financial assistance in the form of “Long-term Foreign Scientist Program”. SKD also acknowledges the financial assistance from INSA-KOSEF Exchange Program.

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Copyright information

© Springer Science+Business Media B.V. 2006

Authors and Affiliations

  • D. Chakrabarty
    • 1
    • 3
  • Y. H. Dewir
    • 2
    • 3
  • E. J. Hahn
    • 3
  • S. K. Datta
    • 1
  • K. Y. Paek
    • 3
  1. 1.Floriculture SectionNational Botanical Research InstituteLucknowIndia
  2. 2.Department of Horticulture, Faculty of AgricultureKafr El Sheikh UniversityKafr El SheikhEgypt
  3. 3.Research Center for the Development of Advanced Horticultural TechnologyChungbuk National UniversityCheong-juKorea

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