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Participation of polyamines in the flowering of the short-day plant Pharbitis nil

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Abstract

The effect of the exogenous application of polyamines on the flowering induction of the short-day plant Pharbtis nil was investigated. Putrescine, spermidine and spermine applied on the cotyledons of 4-day seedlings had no significant effect on the flowering of this plant under conditions of full induction caused by a 16-hour-long inductive night. Under the conditions of partial induction caused by a 13-hour-long subinductive night, polyamines inhibit or stimulate flowering, depending on the time of application. Also, inhibitors of the biosynthesis of polyamines influenced the flowering process. Analysis of endogenous polyamines revealed significant fluctuations in their content in cotyledons during an inductive night, as well as under continuous light conditions. Particularly large changes occurred in spermidine and spermine levels. The putrescine level in induced seedlings was lower than in non-induced ones. However, induced seedlings contained a higher level of spermine and spermidine. The highest spermidine and spermine levels were observed at the 8th h of the night, although the total concentration of spermine during photoinduction was always 2–3 times lower than that of spermidine. A break in the inductive night, leading to a complete inhibition of flowering, had caused significant changes in the polyamine level by the end of the night. The results suggest that the flowering induction of Pharbitis nil took place at a low putrescine level and increased spermidine and spermine levels.

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Abbreviations

CHA:

Cyclohexylamine

DFMO:

Difluoromethylornithine

MGBG:

Methylglyoxal bis-(guanylhydrazone)

PAs:

Polyamines

Put:

Putrescine

SAM:

S-adenosylmethionine

SAMDC:

S-adenosylmethionine decarboxylase

Spd:

Spermidine

Spm:

Spermine

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Acknowledgements

This research was supported by grant No 3P04C 051 24 from the National Committee for Scientific Research (KBN).

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Authors and Affiliations

  1. Didactical Laboratory, Institute of General and Molecular Biology, Nicolaus Copernicus University, 9 Gagarina Street, 87-100, Torun, Poland

    Marlena Zielińska

  2. Department of Physiology and Molecular Biology of Plants, Institute of General and Molecular Biology, Nicolaus Copernicus University, 9 Gagarina Street, 87-100, Torun, Poland

    Jacek Kęsy & Jan Kopcewicz

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  1. Marlena Zielińska
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  2. Jacek Kęsy
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  3. Jan Kopcewicz
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Correspondence to Jacek Kęsy.

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Zielińska, M., Kęsy, J. & Kopcewicz, J. Participation of polyamines in the flowering of the short-day plant Pharbitis nil . Plant Growth Regul 50, 149–158 (2006). https://doi.org/10.1007/s10725-006-9111-9

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