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Auxin pretreatment promotes regeneration of sugarcane (Saccharum spp. hybrids) midrib segment explants

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Abstract

We have developed a new, simple, quick and genotype-independent method for direct regeneration of sugarcane using novel midrib segment explants. Our protocol involves two steps: the pretreatment of starting material on MS (Murashige and Skoog (1962) Physiol Plant 15:473–497) medium containing 3.0 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D) for 8 days under continuous dark and subsequent transfer of the explants to MS medium augmented with 0.1 mg/l benzyladenine (BA) and 0.1 mg/l naphthaleneacetic acid (NAA) under light-dark conditions. On the regeneration medium, numerous globular structures appeared from the explants and subsequently differentiated into shoots. Regenerated shoots attained 2–5 cm height within 30 days of culture initiation and readily rooted on MS basal medium. Hardened plants were successfully established in the greenhouse. The regulation of sugarcane morphogenesis by auxin pretreatment is discussed.

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Abbreviations

BA:

6-Benzyladenine

2,4-D:

2,4-Dichlorophenoxyacetic acid

MS:

Murashige & Skoog

NAA:

Naphthaleneacetic acid

PGR:

Plant Growth Regulator

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Authors and Affiliations

  1. Department of Biology, Universidade do Minho, Campus de Gualtar, 4710-057, Braga, Portugal

    G. Franklin & C. J. Sheeba

  2. Crop Improvement Division, Sugarcane Breeding Institute (ICAR), Coimbatore, 641 007, India

    S. Arvinth, M. Kanchana & N. Subramonian

Authors
  1. G. Franklin
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  2. S. Arvinth
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  3. C. J. Sheeba
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  4. M. Kanchana
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  5. N. Subramonian
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Correspondence to G. Franklin.

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Franklin, G., Arvinth, S., Sheeba, C.J. et al. Auxin pretreatment promotes regeneration of sugarcane (Saccharum spp. hybrids) midrib segment explants. Plant Growth Regul 50, 111–119 (2006). https://doi.org/10.1007/s10725-006-9108-4

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  • DOI: https://doi.org/10.1007/s10725-006-9108-4

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