Abstract
Adventitious shoot regeneration via callus phase from in vitro leaf explants is reported for the first time in tea. Callus was obtained on Murashige and Skoog medium supplemented with varied concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) (2.5, 5.0, 7.5 and 10.0 mg/l). Rhizogenesis was observed at all concentrations of 2,4-D. Adventitious shoot buds developed indirectly on leaf explants after prolonged culture for 16 weeks on medium supplemented with 10.0 mg/l 2,4-D. GC analysis of the medium and the tissues at different stages of development showed that specific levels of 2,4-D in the tissue were responsible for morphogenesis. Shoot buds developed on rhizogenic calli, only when 2,4-D declined to undetectable or negligible concentrations in the tissue probably due to detoxification and metabolism. Alternatively, shoot buds could also be evoked when rhizogenic calli were transferred to medium supplemented with low concentration of 2,4-D (1.5 mg/l). The adventitious nature of the shoots was confirmed through histological studies.
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Abbreviations
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- BA:
-
6-benzyladenine
- IAA:
-
indole-3-acetic acid
- IBA:
-
indole-3-butyric acid
- MS:
-
Murashige and Skoog (1962) medium
- NAA:
-
naphthalene acetic acid
- TDZ:
-
thidiazuron (N-phenyl-N-1,2,3-thiadiazol-5-yl urea)
- PGR:
-
plant growth regulator
References
G.C. Abraham K. Rahman (1986) Somatic embryogenesis in tissue culture of immature cotyledons of tea (Camellia spp.) D.A. Somers B.G. Gengenbach D.D. Biesboer W.P. Hackett C.E. Green (Eds) Abstr. 6th International Congress of Plant Tissue and Cell Culture University of Minnesota Minneapolis 294 pp
Z. Bano S. Rajarathnam B.D. Mohanty (1991) ArticleTitleSomatic embryogenesis in cotyledon culture of tea (Thea sinensis L.) J. Hort. Sci. 66 465–470
A. Bhattacharya P.S. Ahuja (2003) Prospects of transgenics in tea crop improvement R.P. Singh P.K. Jaiwal (Eds) Plant Genetic Engineering. Improvement of Commercial Plants -I Scientific Technology Publishers LLC, USA
Chkanikov D.I. and Pavlova N.N. 1966. Proteins responsible for 2,4-D detoxication in resistant plants. Agrokhimiya 5: 115–119.
A.W. Dodd (1994) ArticleTitleTissue culture of tea (Camellia sinensis (L.) O. Kuntze – a review Int. J. Trop. Agric. XII IssueID3 & 4 212–247
C.S. Feung R.H. Hamilton R.O. Mumma (1975) ArticleTitleMetabolism of 2,4-D VII. Comparison of metabolites from five species of plant callus tissue cultures J. Agric. Food Chem. 23 IssueID3 373–376 Occurrence Handle10.1021/jf60199a065 Occurrence Handle1150979 Occurrence Handle1:CAS:528:DyaE28XhsVGrurw%3D
J. Jankun S.H. Selman R. Swiercz E. Skrzypczak-Jankun (1997) ArticleTitleWhy drinking green tea could prevent cancer Nature 387 561 Occurrence Handle10.1038/42381 Occurrence Handle9177339 Occurrence Handle1:CAS:528:DyaK2sXktFCisbg%3D
T.B. Jha S. Jha S.K. Sen (1992) ArticleTitleSomatic embryogenensis from immature cotyledons of an elite Darjeeling tea clone Plant Sci. 84 209–213 Occurrence Handle10.1016/0168-9452(92)90136-A Occurrence Handle1:CAS:528:DyaK38XltVygs7k%3D
M. Kato (1982) ArticleTitleResult of organ culture on Camellia japonica C. sinensis Jpn. J. Breed. 32 (Supplement 2) 267–277
M. Kato (1996) ArticleTitleSomatic embryogenesis from immature leaves of in vitro grown tea shoots Plant Cell Rep. 15 920–926 Occurrence Handle1:CAS:528:DyaK28Xmt1art7c%3D
K.B. Koens (1993) The growth regulator 2,4-D as a signal molecule in tobacco cell cultures Leiden University Leiden, The Netherlands
K.B. Koens F.T. Nicoloso T.B. VanVliet M. Harteveld C.J.M. Boot F. VanIren K.R. Libbenga J.W. Kijne (1995) ArticleTitleKinetics of 2,4-dichlorophenoxyacetic acid content in an auxin-dependant suspension culture of Nicotiana tabaccum cells J. Plant Physiol. 147 383–390 Occurrence Handle1:CAS:528:DyaK28XnvVegug%3D%3D
M. Kreuger E. Postma Y. Brouwer G.J. Van Holst (1995) ArticleTitleSomatic embryogenesis of Cyclamen persicum in liquid medium Physiol. Plant. 94 605–612 Occurrence Handle10.1034/j.1399-3054.1995.940410.x Occurrence Handle1:CAS:528:DyaK2MXpt1Kqt78%3D
J.J. Leguay J. Guern (1977) ArticleTitleQuantitative effects of 2,4-dichlorophenoxyacetic acid on growth of suspension-cultured Acer pseudoplatanus cells Plant Physiol. 60 265–270 Occurrence Handle1:CAS:528:DyaE2sXlvFGgsbY%3D Occurrence Handle10.1104/pp.60.2.265 Occurrence Handle16660072
E.A. Meijer S.C. DeVries A.P. Mordhorst (1999) ArticleTitleCo-culture with Daucus carrota somatic embryos reveals high uptake and release rates of Arabidopsis thaliana cultured cells Plant Cell Rep. 18 656–663 Occurrence Handle10.1007/s002990050638 Occurrence Handle1:CAS:528:DyaK1MXitVOlsrY%3D
S.A. Merkle W.A. Parrott E.G. Williams (1990) Applications of somatic embryogenesis and embryo cloning S.S. Bhojwani (Eds) Developments in Crop Science 1 Plant Tissue Culture: Applications and Limitation Elsevier Amsterdam 67–101
T. Mitsuoka H. Honda X.H. Xing H. Unno (1994) ArticleTitleEffect of intracellular 2,4-D concentration on plantlet regeneration of rice (Oryza sativa L.) callus Appl. Microbiol. Biotechnol. 42 364–366 Occurrence Handle1:CAS:528:DyaK2MXjvFKktb4%3D
T.K. Mondal A. Bhattacharya A. Sood P.S. Ahuja (1998) ArticleTitleMicropropagation of tea Camellia sinensis (L.) O. Kuntze) using thidiazuron Plant Growth Regul. 26 57–61 Occurrence Handle10.1023/A:1006019206264 Occurrence Handle1:CAS:528:DyaK1cXntlemt7w%3D
T.K. Mondal A. Bhattacharya M. Lakshmikumaran P.S. Ahuja (2004) ArticleTitleRecent advances in tea biotechnology Plant Cell Tiss. Org. Cult. 76 IssueID3 195–254 Occurrence Handle10.1023/B:TICU.0000009254.87882.71 Occurrence Handle1:CAS:528:DC%2BD3sXpvVGgt7Y%3D
T. Murashige F.A. Skoog (1962) ArticleTitleA revised medium for rapid growth and bioassay with tobacco tissue cultures Physiol. Plant. 15 473–497 Occurrence Handle1:CAS:528:DyaF3sXksFKm
Y. Nakamura (1984) ArticleTitleCultivation and characteristics of tea callus Tea Res. J. 60 7–14
Y. Nakamura (1985) ArticleTitleEffect of origin of explants on differentiation of root and its varietal difference in tissue culture of tea plant Tea Res. J. 62 1–8
I. Sandal A. Bhattacharya P.S. Ahuja (2001) ArticleTitleAn efficient liquid culture system for tea shoot proliferation Plant Cell Tiss. Organ Cult. 65 75–80 Occurrence Handle10.1023/A:1010662306067 Occurrence Handle1:CAS:528:DC%2BD3MXlt1Srs7w%3D
T.M. Sarathchandra P.D. Upali R.G.A. Wijevardena (1988) ArticleTitleStudies on the tissue culture of tea Camellia sinensis (L.) O. Kuntze 4. Somatic embryogenesis in stem and leaf callus cultures SL. J. Tea Sci. 52 50–54
M. Sarwar (1985) ArticleTitleCallus formation from explanted organs of tea (Camellia sinensis L.) J. Tea Sci. 54 18–22
Y.J. Wang Y. Xuecheng J. ShuYi W. XinJie (1994) ArticleTitleHisto-cytological study on somatic embryogenesis from the leaf of Camellia sinensis J. Trop. Sub-Trop. Bot. 2 47–51
Wilcox M., Moreland D.E. and Klingman G.C. 1963. Aryl hydroxylation of phenoxy-aliphatic acids by excised roots. Physiol. Plant. 16: 565–571.
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Sandal, I., Kumar, A., Bhattacharya, A. et al. Gradual Depletion of 2,4-D in the Culture Medium for Indirect Shoot Regeneration from Leaf Explants of Camellia sinensis (L.) O. Kuntze. Plant Growth Regul 47, 121–127 (2005). https://doi.org/10.1007/s10725-005-3254-y
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DOI: https://doi.org/10.1007/s10725-005-3254-y