Skip to main content
SpringerLink
Log in

Effects of Short-term Red Radiation and Choline Compounds on Cytokinin Content, Chlorophyll Accumulation and Photomorphogenesis in Wheat Seedlings

  • Published:
Plant Growth Regulation Aims and scope Submit manuscript

Abstract

Effects of choline compounds (2-chloroethyltrimethylammonium chloride and 2-ethyltrimethylammonium chloride) as well as red radiation (R) pulse on the dynamics of cytokinin changes, growth and chlorophyll (a + b) accumulation were studied during the growth and greening of etiolated wheat seedlings (Triticum aestivum L., var. Mironovskaya-808). The seedlings were grown for 120 h in the dark and then exposed for 72 h to white light. Pre-treatment of caryopses with cholines and pre-irradiation of etiolated seedlings with R inhibited elongation of both coleoptile and first leaf; but the same factors accelerated these growth responses when seedlings were exposed to white light. Chlorophyll (Chl) accumulation and the first leaf appearance from coleoptile were accelerated by the pre-treatments as well. Far-red radiation (FR) reversed all effects of R but choline pre-treatment eliminated partly R/FR photoreversibility. Two compounds with high cytokinin activity (tested on a fresh weight basis by the bioassay with Amaranthus caudatus L.) were found in shoots and first leaves. One of them had Rf, UV absorbance spectrum and the biological activity similar to N6-(Δ2-isopentenyl)adenosine. Another cytokinin-like substance was not identified with the used standards. Stimulation of greening by R pulse and cholines was accompanied with accelerated accumulation of both cytokinin-like substances. We conclude that the influence of R and cholines on the concentration of substances with cytokinin activities detected in the leaves might be involved in the stimulation of Chl accumulation.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

CC:

2-ethyltrimethylammonium chloride (choline chloride)

CCC:

(2-chloroethyltrimethylammonium chloride (chlorocholine chloride)

Chl:

chlorophyll (a + b)

Cyt:

cytokinin-like substance

FR:

far-red radiation

iP:

N6-(Δ2-isopentenyl)adenine

iPA:

N6-(Δ2-isopentenyl)adenosine

R:

red radiation

Z:

zeatin

ZR:

zeatin riboside

References

  • G. Banowetz (1997) ArticleTitleCultivars of hexaploid wheat of contrasting stature and chlorophyll retention differ in cytokinin content and responsiveness Ann. Bot. 79 185–190 Occurrence Handle10.1006/anbo.1996.0325

    Article  Google Scholar 

  • F.J. Behringer P.J. Davies J.B. Reid (1990) ArticleTitleGenetic analysis of the role of gibberellins in the red light inhibition of stem elongation in etiolated seedlings Plant Physiol. 94 432–439

    Google Scholar 

  • N.L. Biddington T.H. Thomas (1973) ArticleTitleA modified Amaranthus betacyanin bioassay for the rapid determination of cytokinins in plant extracts Planta 111 183–186 Occurrence Handle10.1007/BF00386279

    Article  Google Scholar 

  • P.H. Blum B.N. Ames (1989) ArticleTitleImmunochemical identification of t-RNA-independent cytokinin-like compound in Salmonella typhimurium Biochem. Biophys. Acta 1007 196–202 Occurrence Handle2645936

    PubMed  Google Scholar 

  • W.R. Briggs E. Mosinger E. Shafer (1988) ArticleTitlePhytochrome regulation of greening in barley-effects on chlorophyll accumulation Plant Physiol. 86 435–440

    Google Scholar 

  • J. Chory D. Reinecke S. Sim T. Washburn M. Brenner (1994) ArticleTitleA role for cytokinins in de-etiolation in Arabidopsis: det mutants have an altered response to cytokinins Plant Physiol. 104 339–347 Occurrence Handle12232085

    PubMed  Google Scholar 

  • J.A. Greef ParticleDe H. Friderico (1983) Photomorphogenesis and Hormones W. Shropshire H. Mohr (Eds) Photomorphogenesis Springer-Verlag Berlin, Heidelberg, New-York, Tokio 401–427

    Google Scholar 

  • V.P. Deeva (1980) Retardants as Regulators of Plant Growth Nauka Tekhnika Minsk

    Google Scholar 

  • A. Evans H. Smith (1976) ArticleTitleLocalization of phytochrome in etioplasts and its regulation in vitro of gibberellin levels Proc. Natl. Acad. Sci. USA. 73 138–142

    Google Scholar 

  • M. Ford H. Kasemir H. Mohr (1981) ArticleTitleThe influence of phytochrome and kinetin on chlorophyll accumulation in mustard cotyledons. A two factor analysis Ber. Dtsch. Bot. Ges. 94 35–41

    Google Scholar 

  • G. Haberer J.J. Kieber (2002) ArticleTitleCytokinins. New insights into classic phytohormone Plant Physiol. 128 354–362 Occurrence Handle10.1104/pp.128.2.354 Occurrence Handle11842139

    Article  PubMed  Google Scholar 

  • P.D. Hare J. Staden ParticleVan (1997) ArticleTitleThe molecular basis of cytokinin action Plant Growth Regul. 3 41–78 Occurrence Handle10.1023/A:1005902508249

    Article  Google Scholar 

  • J.I. Kim G.V. Kozhukh P.S. Song (2002) ArticleTitlePhytochrome-mediated signal transduction pathways in plants Biochem. Biophys. Res. Commun. 298 457–63 Occurrence Handle10.1016/S0006-291X(02)02494-4 Occurrence Handle12408973

    Article  PubMed  Google Scholar 

  • E.F. Kobzar V.D. Kreslavskii E.N. Muzafarov (1999) ArticleTitleRed radiation and choline compounds influence growth and greening of wheat seedlings Photosynthetica 36 333–340 Occurrence Handle10.1023/A:1007029402368

    Article  Google Scholar 

  • G.R. Kudoyarova R.G. Farhutdinov A.N. Mitrichenko I.R. Teplova A.V. Dedov S.U. Veselov O.N. Kulaeva (1998) ArticleTitleFast changes in growth rate and cytokinin content of the shoot following rapid cooling of wheat seedling Plant Growth Regul. 26 105–108 Occurrence Handle10.1023/A:1006088827358

    Article  Google Scholar 

  • E.D. Kuznetsov V.F. Vasilenko V.D. Kreslavsky (1992) ArticleTitleStimulation effects of short-term red light and plant growth retardants on greening and formation of photosynthetic apparatus Plant Physiol. Biochem. 30 559–564

    Google Scholar 

  • V.G. Ladygin G.N. Shirshikova G.A. Semenova V.D. Kreslavski (2002) ArticleTitleEffect of choline chloride on chloroplast ultrastructure and cell growth of green alga Chlamydomonas reingardtii Biophizika 46 256–264

    Google Scholar 

  • H.K Lichtenthaler A.R. Wellburn (1983) ArticleTitleDetermination of total carotenoids and chlorophylls a and b of leaf extracts in different solvents Biochem. Soc. Trans. 11 591–592

    Google Scholar 

  • G.A. Miura T.M. Shih (1984) ArticleTitleCholinergic constituents in plants: characterization and distribution of acetylcholine and choline Physiol. Plant. 61 417–421

    Google Scholar 

  • Mohr H. 1984. Phytochrome and chloroplast development. In: Baker N.R. and Barber J. (eds.) Chloroplast Biogenesis. Elsevier Science Publishers B.V., pp. 307–345.

  • S.K. Nandi D.S. Letham L.M. Palni O.S. Wang R.E. Summons (1989) ArticleTitle6-Benzylaminopurine and its glycosides as naturally occurring cytokinins Plant Sci. 61 189–196 Occurrence Handle10.1016/0168-9452(89)90223-9

    Article  Google Scholar 

  • P.H. Quail (1994) ArticleTitlePhotosensory perception and signal transduction in plants Curr. Opin. Genet. Dev. 6 613–28

    Google Scholar 

  • E.S. Ron’zhina (2003) ArticleTitleCytokinin control of cell mesophyll division and elongation during leaf ontogenesis Russ. J. Plant Physiol. 50 IssueID5 722–732 Occurrence Handle10.1023/B:RUPP.0000003269.31706.ed

    Article  Google Scholar 

  • H. Smith G. Whitelam (1990) ArticleTitlePhytochrome is a family of photoreceptors with multiple physiological roles Plant Cell Environ. 130 695–707

    Google Scholar 

  • T.N. Thomas P.D. Hare J. Staden ParticleVan (1997) ArticleTitlePhytochrome and cytokinin responses Plant Growth Regul. 23 105–122 Occurrence Handle10.1023/A:1005906609158

    Article  Google Scholar 

  • V.F. Vasilenko V.D. Kreslavski E.D. Kuznetsov (1991) ArticleTitleChlorocholine chloride as a modifier of number of phytochrome-controlled processes of growth and photosynthesis Dokl. Acad. Nauk SSSR 316 1512–1514

    Google Scholar 

  • A. Arnim ParticleVon X-W. Deng (1996) ArticleTitleLight control of seedling development Annu. Rev. Plant Physiol. Plant Mol. Biol. 47 215–243 Occurrence Handle10.1146/annurev.arplant.47.1.215 Occurrence Handle15012288

    Article  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Basic Biological Problems of Russian Academy of Sciences, 142290, Pushchino, Moscow Region, Russia

    V. Kreslavski, E. Kobzar & E. Ivanova

  2. Institute of General Physics of Russian Academy of Sciences, Moscow, Russia

    E. Kuznetsov

Authors
  1. V. Kreslavski
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. E. Kobzar
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. E. Ivanova
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. E. Kuznetsov
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to V. Kreslavski.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kreslavski, V., Kobzar, E., Ivanova, E. et al. Effects of Short-term Red Radiation and Choline Compounds on Cytokinin Content, Chlorophyll Accumulation and Photomorphogenesis in Wheat Seedlings. Plant Growth Regul 47, 9–15 (2005). https://doi.org/10.1007/s10725-005-0861-6

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10725-005-0861-6

Key words

Search

Navigation