Genetic diversity of Aegilops L. species from Azerbaijan and Georgia using SSR markers
- 292 Downloads
Five microsatellite (SSR) markers were used to evaluate the genetic diversity of six Aegilops species from Azerbaijan and Georgia. A total of 39 alleles were generated with an average of 7.8 alleles per primer. Twenty markers were species-specific and 6 were accession-specific. The transferability of SSR markers across six species was 100%, with exception of gwm210. The mean polymorphism information content (PIC) and expected heterozygosity (He) values for the entire collection were 0.688 and 0.725, respectively. The average PIC value was the highest in Ae. biuncialis accessions (0.55). The genetic distance (GD) indices, based on five SSR markers, ranged from 0 to 0.83, with a mean value of 0.47. The highest genetic similarity was noted between Ae. neglecta and Ae. triuncialis (GD = 0.26), and the lowest between Ae. neglecta and Ae. tauschii (GD = 0.66). The dendrogram created based on SSR data grouped 72 Aegilops accessions into six clusters according to their taxonomic classification. The accessions from the same province were often placed in the same subclusters, indicating that grouping based on genetic parameters was closely related to the geographic region within countries. The PCoA analysis could differentiate Aegilops accessions according to their species and confirmed subgrouping obtained by cluster analysis.
KeywordsAegilops Species SSR Genetic diversity Genetic relationship
Funding was provided by Norman Borlaug Fellowship, Fulbright fellowship.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
- Abbasov M, Akparov Z, Gross T, Babayeva S, Izzatullayeva V, Hajiye E, Rustamov K, Gross P, Tekin M, Akar T, Chao S (2018) Genetic relationship of diploid wheat (Triticum spp.) species assessed by SSR markers. Genet Resour Crop Evol:1–13Google Scholar
- Aliyev RT, Abbasov MA, Mammadov AC (2007) Genetic identification of diploid and tetraploid wheat species with RAPD markers. Turk J Biol 31(3):173–180Google Scholar
- Alnaddaf LM, Moualla MY, Haider N (2012) The Genetic Relationships among Aegilops L. and Triticum L. species. Asian J Agric Sci 4(5):352–367Google Scholar
- Dvorak J, Luo MC, Yang ZL (1998) Genetic evidence on the origin of Triticum aestivum L. In: Damania AB, Valkoun J, Willcox G, Qualset CO (eds) The origins of agriculture and crop domestication. Proceedings of Harlan symposium. ICARDA, Aleppo, pp 235–251Google Scholar
- Gascuel O (1997) Concerning the NJ algorithm and its unweighted version, UNJ. In: Mathematical hierarchies and biology. DIMACS workshop, Series in Discrete Mathematics and Theoretical Computer Science. American Mathematical Society vol 37, pp 149–170Google Scholar
- Hedge SG, Valkoun J, Waines JG (2002) Genetic diversity in wild and weedy Aegilops, Amblyopyrum and Secale species: preliminary survey. Crop Sci 42:608–614Google Scholar
- Karaca M, Ince AG (2011) New non-redundant microsatellite and CAPS-microsatellite markers for cotton (Gossypium L.). Turk J Field Crops 16:172–178Google Scholar
- Karcicio M, Izbirak A (2003) Isozyme variations in some Aegilops L. and Triticum L. species collected from Central Anatolia. Turk J Bot 27(6):433–440Google Scholar
- Kilian B, Mammen K, Millet E, Sharma R, Graner A, Salamini F, Hammer K, Özkan H (2011) Aegilops. Wild crop relatives: genomic and breeding resources. Springer, Berlin, pp 1–76Google Scholar
- Naghavi MR, Aghaei MJ, Taleei AR, Omidi M, Hassani ME (2008) Genetic diversity of hexaploid wheat and three Aegilops species using microsatellite markers. https://ses.library.usyd.edu.au/bitstream/2123/3231/1/P028.pdf. Accessed 18 Nov 2018
- Perrier X, Jacquemoud-Collet JP (2006) DARwin software. http://darwin.cirad.fr/darwin. Accessed 18 Nov 2018
- Sears ER (1956) The transfer of leaf rust resistance from Aegilops umbellulata to wheat. Brookhaven Symp Biol 9:1–22Google Scholar
- Van Slageren MW (1994) Wild wheats: a monograph of Aegilops L. and Amblyopyrum (Jaub. & Spach) Eig (Poaceae). Agricultural University Papers, Wageningen, NetherlandsGoogle Scholar
- Zhang XY, Wang RRC, Dong YS (1996) RAPD polymorphisms in Aegilops geniculata Roth (Ae. ovata auct. non L.). Genet Resour Crop Evol 43:429–433Google Scholar