Abstract
Citron watermelon (Citrullus lanatus var. citroides) is an important tropical crop belonging to the Cucurbitaceae family. It possesses novel genes for biotic and abiotic stress tolerance and horticultural traits useful for breeding including sweet dessert watermelon. There is a lack of research priority limiting the genetic improvement and cultivar deployment of citron watermelon. Genetically diverse citron watermelon genetic resources are important to identify and select suitable genotypes for cultivar development incorporating horticultural attributes according to the needs and preferences of clients and marketplace. The objective of this review was to present progress on genetic improvement of citron watermelon with emphasis on cultivar development with biotic and abiotic stress resistance and desired horticultural attributes. Further, the review highlighted genetic and molecular resources in citron watermelon and other cross compatible cucurbits that may aid in accelerated breeding and cultivar release. Finally, the paper summarized research focus areas with potential to enhance effective utilization of citron watermelon genetic resources for targeted breeding of this crop or related taxa such as dessert watermelon.
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Achigan-Dako EG, Avohou ES, Linsoussi C, Ahanchede A, Vodouhe RS, Blattner FR (2015) Phenetic characterization of Citrullus spp. (Cucurbitaceae) and differentiation of egusi-type (C. mucosospermus). Genet Resour Crop Evol 62:1159–1179
Akashi K, Miyake C, Yokota A (2001) Citrulline, a novel compatible solute in drought-tolerant wild watermelon leaves, is an efficient hydroxyl radical scavenger. FEBS Lett 508:438–442
Bang H, Kim S, Leskovar D, King S (2007) Development of a codominant CAPS marker for allelic selection between canary yellow and red watermelon based on SNP in lycopene b-cyclase (LCYB) gene. Mol Breed 20:63–72
Bang H, Davis AR, Kim S, Leskovar DI, King SR (2010) Flesh color inheritance and gene interactions among canary yellow, pale yellow, and red watermelon. J Am Soc Hortic Sci 135:362–368
Ben-Naim Y, Cohen Y (2015) Inheritance of resistance to powdery mildew race 1 W in watermelon. Phytopathology 105:1446–1457
Boyhan G, Norton JD, Abrahams BR (1994) Screening for resistance to anthracnose (race 2), gummy stem blight, and root knot nematode in watermelon germplasm. Cucurbit Genet Coop 17:106–110
Boyhan GE, Langston DB, Granberry DM, Lewis PM, Linton DO (2003) Resistance to Fusarium wilt and root-knot nematode in watermelon germplasm. Cucurbit Genet Coop Rep 26:18–25
Branham SE, Vexler L, Meir A, Tzuri G, Frieman Z, Levi A, Wechter WP, Tadmor Y, Gur A (2017a) Genetic mapping of a major codominant QTL associated with β-carotene accumulation in watermelon. Mol Breed 37:146
Branham SE, Levi A, Farnham MW, Wechter P (2017b) A GBS-SNP-based linkage map and quantitative trait loci (QTL) associated with resistance to Fusarium oxysporum f. sp. niveum race 2 identified in Citrullus lanatus var. citroides. Theor Appl Genet 130:319–330
Brotman Y, Kovalski I, Dogimont C, Pitrat M, Portnoy V, Katzir N, Perl-Treves R (2005) Molecular markers linked to papaya ring spot virus resistance and Fusarium race 2 resistance in melon. Theor Appl Genet 110:337–345
Cheng Y, Luan F, Wang X, Gao P, Zhu Z, Liu S, Baloch AM, Zhang Y (2016) Construction of a genetic linkage map of watermelon (Citrullus lanatus) using CAPS and SSR markers and QTL analysis for fruit quality traits. Sci Hortic 202:25–31
Choi O, Park JJ, Kim J (2016) Tetranychus urticae (Acari: Tetranychidae) transmits Acidovorax citrulli, causal agent of bacterial fruit blotch of watermelon. Exp Appl Acarol 69:445–451
Chomicki G, Renner SS (2015) Watermelon origin solved with molecular phylogenetics including Linnaean material: another example of museomics. New Phytol 205:526–532
Cohen R, Tyutyunik J, Fallik E, Oka Y, Tadmor Y, Edelstein M (2014) Phytopathological evaluation of exotic watermelon germplasm as a basis for rootstock breeding. Sci Hortic 165:203–210
Dane F, Lang P (2004) Sequence variation at cpDNA regions of watermelons and related wild species: implications for evolution of Citrullus haplotypes. Amer J Bot 91:1922–1929
Dane F, Liu J (2007) Diversity and origin of cultivated and citron type watermelon (Citrullus lanatus). Genet Resour Crop Evol 54:1255–1265
Dane F, Hawkins LK, Norton JD, Kwon YS, Om YH (1998) New resistance to race 2 of Fusarium oxysporum f.sp. niveum in watermelon. Cucurbit Genet Coop Rep 21:37–39
Davis AR, Collins J, Fish WW, Tadmor Y, Webber CL, Perkins-Veazie P (2007) Rapid method for total carotenoid detection in canary yellow-fleshed watermelon. J Food Sci 72:S319–S323
Davis A, Perkins-Veazie P, Collins J, Levi A (2008) LSW-177 and LSW-194: red-fleshed watermelon lines with low total soluble solids. HortScience 43:538–539
Dou J, Zhao S, Lu X, He N, Zhang L, Ali A, Kuang H, Liu W (2018) Genetic mapping reveals a candidate gene (ClFS1) for fruit shape in watermelon (Citrullus lanatus L.). Theor Appl Genet 131:947–958
Edelstein M, Tyutyunik J, Fallik E, Meir A, Tadmor Y, Cohen R (2014) Horticultural evaluation of exotic watermelon germplasm as potential rootstocks. Sci Hortic 165:196–202
Fox FW, Norwood ME (1982) Food from the veld. Delta Books, Johannesburg
Fredes A, Roselló S, Beltrán J, Cebolla-Cornejo J, Pérez-de-Castro A, Gisbert C, Picó MB (2017) Fruit quality assessment of watermelons grafted onto citron melon rootstock. J Sci Food Agric 97:1646–1655
Gillaspie AG, Wright JM (1993) Evaluation of Citrullus sp. germplasm for resistance to watermelon mosaic virus 2. Plant Dis 77:352–354
Guner N (2004) Papaya Ringspot Virus Watermelon Strain and Zucchini Yellow Mosaic Virus resistance in watermelon. PhD Thesis, Department of Horticultural Science, North Carolina State University, Raleigh, pp 257
Guo S, Liu J, Zheng Y, Huang M, Zhang H, Gong G, He H, Ren Y, Zhong S, Fei Z, Xu Y (2011) Characterization of transcriptome dynamics during watermelon fruit development: sequencing, assembly, annotation and gene expression profiles. BMC Genom 12:454
Guo S, Zhang J, Sun H, Salse J, Lucas WJ, Zhang H, Zheng Y, Mao L, Ren Y, Wang Z (2013) The draft genome of watermelon (Citrullus lanatus) and resequencing of 20 diverse accessions. Nat Genet 45:51–58
Guo S, Sun H, Zhang H, Liu J, Ren Y, Gong G, Jiao C, Zheng Y, Yang W, Fei Z, Xu Y (2015) Comparative transcriptome analysis of cultivated and wild watermelon during fruit development. PLoS ONE 10:e0130267
Gusmini G, Wehner TC (2005) Genes determining rind pattern inheritance in watermelon: a review. HortScience 40:1928–1930
Gusmini G, Wehner TC (2006) Qualitative inheritance of rind pattern and flesh color in watermelon. J Hered 97:177–185
Gusmini G, Wehner TC (2007) Heritability and genetic variance estimates for fruit weight in watermelon. HortScience 42:1332–1336
Gusmini G, Song G, Wehner TC (2005) New sources of resistance to Gummy stem blight in watermelon. Crop Sci 45:582–588
Gusmini G, Rivera-Burgos LA, Wehner TC (2017) Inheritance of resistance to gummy stem blight in watermelon. HortScience 52:1477–1482
Guzzon F, Müller JV, do Nascimento Araujo M, Cauzzi P, Orsenigo S, Mondoni A, Abeli T (2017) Drought avoidance adaptive traits in seed germination and seedling growth of Citrullus amarus landraces. S Afr J Bot 113:382–388
Harris KR, Ling KS, Wechter WP, Levi A (2009) Identification and utility of markers linked to the Zucchini Yellow Mosaic Virus resistance gene in watermelon. J Am Soc Hortic Sci 134:529–534
Hashizume T, Shimamoto I, Harusima YM, Sato T, Imai T, Hirai M (1996) Construction of a linkage map for watermelon (Citrullus lanatus) using random amplified polymorphic DNA (RAPD). Euphytica 90:265–273
Hashizume T, Shimamoto I, Hirai M (2003) Construction of a linkage map and QTL analysis of horticultural traits for watermelon [Citrullus lanatus (Thumb.) Matsum & Nakai] using RAPD, RFLP and ISSR markers. Theor Appl Genet 106:779–785
Hawkins LK, Dan F, Kubisiak TL (2001a) Molecular markers associated with morphological traits in watermelon. HortScience 36:1318–1322
Hawkins LK, Dane F, Kubisiak TL, Rhodes BB, Jarret RL (2001b) Linkage mapping in a watermelon population segregating for fusarium wilt resistance. J Am Soc Hortic Sci 126:344–350
Hawkins LK, Dane F, Kubisiak TL, Rhodes BB, Jarret RL (2001c) Linkage mapping in a watermelon population segregating for Fusarium wilt resistance. J Am Soc Hort Sci 126:344–350
Holkar SK, Choudhary H, Mandal B, Jain RK (2018) Evaluation of watermelon genotypes against bud necrosis disease caused by a distinct watermelon bud necrosis orthotospovirus under field and glasshouse conditions. Sci Hortic 235:106–115
Hu JB, Zhou XY, Li JW (2010) Development of novel EST–SSR markers for cucumber (Cucumis sativus) and their transferability to related species. Sci Hortic 125:534–538
Hwang JH, Ahn SG, Oh JY, Choi YW, Kang JS, Park YH (2011) Functional characterization of watermelon (Citrullus lanatus L.) EST–SSR by gel electrophoresis and high resolution melting analysis. Sci Hortic 130:715–724
Jarret RL, Merrick LC, Holms T, Evans J, Aradhya MK (1997) Simple sequence repeats in watermelon (Citrullus lanatus (Thunb.) Matsum. & Nakai). Genome 40:433–441
Ji Y, Luo Y, Hou B, Wang W, Zhao J, Yang L, Xue Q, Ding X (2012) Development of polymorphic microsatellite loci in Momordica charantia (Cucurbitaceae) and their transferability to other cucurbit species. Sci Hortic 140:115–118
Joobeur T, Gusmini G, Zhang X, Levi A, Xu Y, Wehner TC, Oliver M, Dean RA (2006) Construction of a watermelon BAC library and identification of SSRs anchored to melon or Arabidopsis genomes. Theor Appl Genet 112:1553–1562
Kang B, Zhao W, Hou Y, Tian P (2010) Expression of carotenogenic genes during the development and ripening of watermelon fruit. Sci Hortic 124:368–375
Kawasaki S, Miyake C, Kohchi T, Fujii S, Uchida M, Yokota A (2000) Responses of wild watermelon to drought stress: accumulation of drought response of wild watermelon roots an ArgE homologue and citrulline in leaves during water deficits. Plant Cell Physiol 42:864–873
Kim KH, Ahn SG, Hwang JH, Choi YM, Moon HS, Park YH (2013) Inheritance of resistance to powdery mildew in the watermelon and development of a molecular marker for selecting resistant plants. Hortic Environ Biotechnol 54:134–140
Kim H, Han D, Kang J, Choi Y, Levi A, Lee GP, Park Y (2015a) Sequence-characterized amplified polymorphism markers for selecting rind stripe pattern in watermelon (Citrullus lanatus L.). Hortic Environ Biotechnol 56:341–349
Kim KH, Hwang JH, Han DY, Park M, Kim S, Choi D, Kim Y, Lee GP, Kim ST, Park YH (2015b) Major quantitative trait loci and putative candidate genes for powdery mildew resistance and fruit-related traits revealed by an intraspecific genetic map for watermelon (Citrullus lanatus var. lanatus). PLoS ONE 10:e0145665
Kousik CS, Adkins S, Turechek WW, Roberts PD (2009) Sources of resistance in US plant introductions to watermelon vine decline caused by squash vein yellowing virus. HortScience 44:256–262
Kousik CS, Ikerd JL, Wechter P, Harrison H, Levi A (2012) Resistance to phytophthora fruit rot of watermelon caused by Phytophthora capsici in US plant introductions. HortScience 47:1682–1689
Laghetti G, Hammer K (2007) The Corsican citron melon (Citrullus lanatus (Thunb.) Matsum. et Nakai subsp. lanatus var. citroides (Bailey) Mansf. ex Greb.) a traditional and neglected crop. Genet Resour Crop Evol 54:913–916
Lambel S, Lanini B, Vivoda E, Fauve J, Wechter PW, Harris-Shultz KR, Massey L, Levi A (2014) A major QTL associated with Fusarium oxysporum race 1 resistance identified in genetic populations derived from closely related watermelon lines using selective genotyping and genotyping-by-sequencing for SNP discovery. Theor Appl Genet 127:2105–2115
Levi A, Thomas CE (2005) Polymorphisms among chloroplast and mitochondrial geneomes of Citrullus species and subspecies. Genet Resour Crop Evol 52:609–617
Levi A, Thomas CE, Keinath AP, Wehner TC (2000) Estimation of genetic diversity among Citrullus accessions using RAPD markers. Acta Hortic 510:385–390
Levi A, Thomas CE, Keinath AP, Wehner TC (2001a) Genetic diversity among watermelon (Citrullus lanatus and Citrullus colocynthis) accessions. Genet Resour Crop Evol 48:559–566
Levi A, Thomas CE, Wehner TC, Zhang X (2001b) Low genetic diversity indicates the need to broaden the genetic base of cultivated watermelon. HortScience 36:1096–1101
Levi A, Thomas CE, Zhang X, Joobeur T, Dean RA, Wehner TC (2001c) A genetic linkage map for watermelon based on randomly amplified polymorphic DNA markers. J Am Hortic Sci 126:730–737
Levi A, Wechter WP, Massey LM, Carter L, Hopkins D (2011) An extended genetic linkage map for watermelon based on a testcross and a BC2F2 population. Am J Plant Sci 2:93–110
Levi A, Thomas E, Joobeur T, Zhang X, Davis A (2002) A genetic linkage map for watermelon derived from a testcross population: (Citrullus lanatus var. citroides × C. lanatus var. lanatus) × Citrullus colocynthis. Theor Appl Genet 105:555–563
Levi A, Thies JA, Wechter WP, Harrison HFJ, Simmons AM, Reddy U, Nimmakayala P, Fei Z (2012) High frequency oligonucleotides—targeting active gene (HFO–TAG) markers reveal wide genetic diversity among Citrullus spp. accessions useful for enhancing disease or pest resistance in watermelon cultivars. Genet Resour Crop Evol 60:427–440
Levi A, Thies JA, Wechter PW, Farnham M, Weng Y, Hassell R (2014) USVL-360, a novel watermelon tetraploid germplasm line. HortScience 49:354–357
Levi A, Jarret R, Kousik S, Wechter PW, Nimmakayala P, Reddy UK (2017) Genetic resources of watermelon. In: Katzir R, Garcia-Mas GNJ (eds) Genetics and genomics of Cucurbitaceae. Plant genetics and genomics: crops and models. Springer, Berlin, pp 87–110
Ling KS, Harris KR, Meyer JDF, Levi A, Guner N, Wehner TC, Bendahmane A, Havey MJ (2009) Non-synonymous single nucleotide polymorphisms in the watermelon eIF4E gene are closely associated with resistance to zucchini yellow mosaic virus. Theor Appl Genet 120:191–200
Liu J, Guo S, He H, Zhang H, Gong G, Ren Y, Xu Y (2013) Dynamic characteristics of sugar accumulation and related enzyme activities in sweet and non-sweet watermelon fruits. Acta Physiol Plant 35:3213–3222
Ma S, Wehner TC (2015) Flowering stage resistance to bacterial blotch in the watermelon germplasm collection. Crop Sci 55:727–736
Maggs-Kölling GL, Christiansen JL (2003) Variability in Namibian landraces of watermelon (Citrullus lanatus). Euphytica 123:251–258
Maggs-Kölling GL, Madsen S, Christiansen JL (2000) A phenetic analysis of morphological variation in Citrullus lanatus in Namibia. Genet Resour Crop Evol 47:385–393
Mashilo J, Shimelis H, Odindo A (2015) Genetic diversity of bottle gourd (Lagenaria siceraria (Molina) Standl.) landraces of South Africa assessed by morphological traits and simple sequence repeat markers. S Afr J Plant Soil 33:113–124
Mashilo J, Shimelis H, Odindo A, Amelework B (2016) Simple sequence repeat markers reveal genetic diversity within and among landrace collections of citron and dessert watermelon from South Africa. J Am Soc Hortic Sci 141:598–608
Mashilo J, Shimelis H, Odindo AO, Amelework BA (2017) Genetic diversity and differentiation in citron watermelon [Citrullus lanatus var. citroides] landraces assessed by simple sequence repeat markers. Sci Hortic 214:99–106
Mcgregor C (2012) Citrullus lanatus germplasm of southern Africa. Isr J Plant Sci 60:403–413
McGregor C, Waters V (2013) Pollen viability of F1 hybrids between watermelon cultivars and diseaseresistant, infraspecific crop wild relatives. HortScience 48:1428–1432
Meru G, McGregor C (2014) Quantitative trait loci associated with fatty acid content of watermelon seed. J Am Soc Hortic Sci 139:433–441
Mo Y, Yang R, Liu L, Gu X, Yang X, Wang Y, Zhang X, Li H (2016) Growth, photosynthesis and adaptive responses of wild and domesticated watermelon genotypes to drought stress and subsequent re-watering. Plant Growth Reg 79:229–241
Mujaju C, Sehic J, Werlemark G, Garkava-Gustavsson L, Fatih M, Nybom H (2010) Genetic diversity in watermelon (Citrullus lanatus) landraces from Zimbabwe revealed by RAPD and SSR markers. Hereditas 147:142–153
Mujaju C, Sehie J, Nybom H (2013) Assessment of EST–SSR markers for evaluating genetic diversity in watermelon accessions from Zimbabwe. Am J Plant Sci 4:1448–1456
Munnisse P, Jensen BD, Andersen SB (2013) Genetic differentiation of watermelon landraces in Mozambique using microsatellite markers. Afr J Biotechnol 12:5513–5521
Nantoumé AD, Andersen SB, Jensen BD (2013) Genetic differentiation of watermelon landrace types in Mali revealed by microsatellite (SSR) markers. Genet Resour Crop Evol 60:2129–2141
Navot N, Zamir D (1987) Isozyme and seed protein phylogeny of the genus Citrullus (Cucrbitaceae). Plant Syst Evol 156:61–76
Nimmakayala P, Tomason YR, Jeong J, Ponniah SK, Karunathilake A, Levi A, Perumal R, Reddy UK (2009) Genetic reticulation and interrelationships among Citrullus species as revealed by joint analysis of shared AFLPs and species-specific SSR alleles. Plant Genet Resour 8:16–25
Nimmakayala P, Levi A, Abburi L, Abburi VL, Tomason YR, Saminathan T, Vajja VG, Malkaram S, Reddy R, Wehner TC, Mitchell SE, Reddy U (2014) Single nucleotide polymorphisms generated by genotyping by sequencing to characterize genome-wide diversity, linkage disequilibrium, and selective sweeps in cultivated watermelon. BMC Genom 15:767
Norton JD, Cosper RD, Smith DA, Rymal KS (1986) ‘AU–Jubilant’ and ‘AU–Producer’ watermelons. HortScience 21:1460–1461
Norton JD, Boyhan GE, Smith DA, Abrahams BR (1993) `AU-golden producer’ Watermelon. HortScience 28:681–682
Norton JD, Boyhan GE, Smith DA, Abrahams BR (1995) `AU-sweet scarlet’ watermelon. HortScience 30:393–394
Ocal N, Akbulut M, Osman Gulsen O, Yetisir H, Solmaz I, Sari N (2014) Genetic diversity, population structure and linkage disequilibrium among watermelons based on peroxidase gene markers. Sci Hortic 176:151–161
Palomares-Rius FJ, Viruel MA, Yuste-Lisbona FJ, López-Sesé AI, Gómez-Guillamón ML (2011) Simple sequence repeat markers linked to QTL for resistance to Watermelon mosaic virus in melon. Theor Appl Genet 123:1207
Perkins-Veazie P, Collins JK, Davis AR, Roberts W (2006) Carotenoid content of 50 watermelon cultivars. J Agric Food Chem 54:2593–2597
Prothro J, Sandlin K, Gill R, Bachlava E, White V, Knapp S, McGregor C (2012) Mapping of the egusi seed trait locus (eg) and quantitative trait loci associated with seed oil percentage in watermelon. J Am Soc Hortic Sci 137:311–315
Provvidenti R (1991) Inheritance of resistance to the Florida strain of zucchini yellow mosaic virus in watermelon. HortScience 26:407–408
Reddy U, Aryal N, Islam-Faridi N, Tomason Y, Levi A, Nimmakayala P (2013) Cytomolecular characterization of rDNA distribution in various Citrullus species using fluorescent in situ hybridization. Gen Res Crop Evol 60:2091–2100
Reddy UK, Abburi L, Abburi VL, Saminathan T, Cantrell R, Vajja VG, Reddy R, Tomason YR, Levi A, Wehner TC, Nimmakayala P (2015) A genome-wide scan of selective sweeps and association mapping of fruit traits using microsatellite markers in watermelon. J Hered 106:166–176
Ren Y, Zhao H, Kou Q, Jiang J, Guo S, Zhang H, Hou W, Zou X, Sun H, Gong G (2012) A high resolution genetic map anchoring scaffolds of the sequenced watermelon genome. PLoS ONE. https://doi.org/10.1371/journal.pone.0029453
Ren Y, McGregor C, Zhang Y, Gong G, Zhang H, Guo S, Sun H, Cai W, Zhang J, Xu Y (2014) An integrated genetic map based on four mapping populations and quantitative trait loci associated with economically important traits in watermelon (Citrullus lanatus). BMC Plant Biol 14:33
Ren Y, Jiao D, Gong G, Zhang H, Guo S, Zhang J, Xu Y (2015) Genetic analysis and chromosome mapping of resistance to Fusarium oxysporum f. sp. niveum (FON) race 1 and race 2 in watermelon (Citrullus lanatus L.). Mol. Breed 35:183–192
Rhee SJ, Han BK, Jang YJ, Sim TY, Lee GP (2015) Contsruction of a genetic linkage map using a frame set of simple sequence repeat and high-resolution melting markers for watermelon (Citrullus spp.). Hortic Environ Biotechnol 56:669–676
Robinson RW (2000) Rationale and methods for producing hybrid cucurbit seed. J New Seeds 1:1–47
Robinson RW, Decker-Walters DS (1997) Cucurbits. CABI Publishing, Cambridge, p 226
Rubatzky VE (2001) Origin, distribution and uses. In: Maynard DN (ed) Watermelons. Characteristics, production and marketing. ASHS Press, Alexandria, pp 21–26
Sandlin K, Prothro J, Heesacker A, Khalilian N, Okashah R, Xiang W, Bachlava E, Caldwell DG, Taylor CA, Seymour DK, White V, Chan E, Tolla G, White C, Safran D, Graham E, Knapp S, Mcgregor C (2012) Comparative mapping in watermelon (Citrullus lanatus (Thunb.) Matsum. et Nakai). Theor Appl Genet 125:1603–1618
Schippers RR (2002) African indigenous vegetables, an overview of cultivated species. Natural Resources International Limited, Aylesford, p 214
Shaik RS, Burrows GE, Urwin NAR, Gopurenko D, Lepschi BJ, Weston LA (2017) The biology, phenology and management of Australian weed-camel melon (Citrullus lanatus (Thunb.) Matsum. and Nakai). Crop Prot 98:222–235
Shang J, Li N, Li N, Xu Y, Ma S, Wang J (2016) Construction of a high-density genetic map for watermelon (Citrullus lanatus L.) based on large-scale SNP discovery by specific length amplified fragment sequencing (SLAF-seq). Sci Hortic 203:38–46
Singh D, Singh R, Sandhu JS, Chunneja P (2017) Morphological and genetic diversity analysis of Citrullus landraces from India and their genetic inter relationship with continentalwatermelons. Sci Hortic 218:240–248
Solmaz I, Sari N (2009) Characterization of watermelon (Citrullus lanatus) accessions collected from Turkey for morphological traits. Genet Resour Crop Evol 56:173–188
Solmaz I, Sari N, Aka-Kacar Y, Yalcin-Mendi NY (2010) The genetic characterization of Turkish watermelon (Citrullus lanatus) accessions using RAPD markers. Genet Resour Crop Evol 57:763–771
Sowell GJ, Rhodes BB, Norton JD (1980) New sources of resistance to watermelon anthracnose. J Am Soc Hort Sci 105:197–199
Strange EB, Guner N, Pesic-Van Esbroeck Z, Wehner TC (2002) Screening the watermelon germplasm collection for resistance to Papaya Ringspot virus type-W. Crop Sci 32:1324–1330
Suvanprakorn K, Norton JD (1980) Inheritance of resistance to race 2 anthracnose in watermelon. J Am Soc Hortic Sci 105:862–865
Szamosi C, Solmaz I, Sari N, Barsony C (2009) Morphological characterization of Hungarian and Turkish watermelon (Citrullus lanatus) (Thunb.) Matsum. et Nakai) genetic resources. Genet Resour Crop Evol 56:1091–1105
Tadmor Y, King S, Levi A, Davis A, Meir A, Wasserman B, Hirschberg J, Lewinsohn E (2005) Comparative fruit colouration in watermelon and tomato. Food Res Inter 38:837–841
Tetteh AY, Wehner TC, Davis AR (2010) Identifying resistance to powdery mildew race 2 W in the USDA-ARS watermelon germplasm collection. Crop Sci 50:933–939
Tetteh AY, Wehner TC, Davis AR (2013a) Inheritance of resistance to powdery mildew race 2 in Citrullus lanatus var. lanatus. HortScience 48:1227–1230
Tetteh AY, Wehner TC, Davis AR (2013b) Inheritance of resistance to the new race of powdery mildew in watermelon. Crop Sci 53:880–887
Thies JA, Levi A (2007) Characterization of watermelon (Citrullus lanatus var. citroides) germplasm for resistance to root-knot nematodes. HortScience 42:1530–1533
Thies JA, Ariss JJ, Hassell RL, Olson S, Kousik CS, Levi A (2010) Grafting for management of Southern root-knot nematode, Meloidogyne incognita, in watermelon. Plant Dis 94:1195–1199
Thies JA, Ariss JJ, Hassell RL, Buckner S, Levi A (2015a) Accessions of Citrullus lanatus var. citroides are valuable rootstocks for grafted watermelon in fields infested with root-knot nematodes. HortScience 50:4–8
Thies JA, Buckner S, Horry M, Hassell R, Levi A (2015b) Influence of Citrullus lanatus var. citroides rootstocks and their F1 hybrids on yield and response to root-knot nematode, Meloidogyne incognita, in grafted watermelon. HortScience 50:9–12
Thies JA, Ariss JJ, Kousik CS, Hassell RL, Levi A (2016) Resistance to Southern root-knot nematode (Meloidogyne incognita) in wild watermelon (Citrullus lanatus var. citroides). J Nematol 48:14–19
Thomas CE, Levi A, Caniglia E (2005) Evaluation of US plant introductions of watermelon for resistance to powdery mildew. HortScience 40:154–156
Wang P, Li Q, Hu J, Su Y (2015) Comparative analysis of genetic diversity among Chinese watermelon germplasms using SSR and SRAP markers, and implications for future genetic improvement. Turk J Agric For 39:322–331
Watcharawongpaiboon N, Chunwongse J (2008) Development and characterization of microsatellite markers from an enriched genomic library of cucumber (Cucumis sativus). Plant Breed 127:74–81
Wechter WP, Kousik C, McMillan M, Levi A (2012) Identification of Resistance to Fusarium oxysporum f. sp. niveum Race 2 in Citrullus lanatus var. citroides plant introductions. HortScience 47:334–338
Wechter WP, McMillan MM, Farnham MW, Levi A (2016) Watermelon germplasm lines USVL246-FR2 and USVL252-FR2 tolerant to Fusarium oxysporum f. sp. niveum Race 2. HortScience 51:1065–1067
Wehner TC (2008) Watermelons. In: Prohens J, Nuez F (eds) Handbook of plant breeding. Vegetables I: Asteraceae, Brassicaceae, Chenopodiaceae, and Cucurbitaceae. Springer, New York, pp 381–418
Wei C, Chen X, Wang Z, Liu Q, Li H, Zhang Y, Ma J, Yang J, Zhang X (2017) Genetic mapping of the LOBED LEAF 1 (ClLL1) gene to a 127.6-kb region in watermelon (Citrullus lanatus L.). PLoS ONE 12:e0180741
Wolukau JN, Zhou X, Chen J (2009) Identification of Amplified Fragment Length Polymorphism Markers linked to gummy stem blight (Didymella bryoniae) resistance in melon (Cucumis melo L.) PI 420145. HortScience 44:32–34
Xu Y, Kang D, Shi Z, Shen H, Wehner T (2004) Inheritance of resistance to zucchini yellow mosaic virus and watermelon mosaic virus in watermelon. J Hered 95:498–502
Yoo KS, Bang H, Lee EJ, Crosby K, Patil BS (2012) Variation of carotenoid, sugar, and ascorbic acid concentrations in watermelon genotypes and genetic analysis. Hortic Environ Biotechnol 53:552–560
Yoshimura K, Masuda A, Kuwano M, Yokota A, Akashi K (2008) Programmed proteome response for drought avoidance/tolerance in the root of a C3 xerophyte (Wild watermelon) under water deficits. Plant Cell Physiol 49:226–241
Zhang XP, Rhodes B (1993) Inheritance of resistance to races 0, 1, and 2 of Fusarium oxysporum f.sp. niveum in watermelon (Citrullus sp. PI 296341). Cucurbit Genet Coop Rep 16:77–78
Zhang R, Xu Y, Yi K, Zhang H, Liu L, Gong G, Levi A (2004) A genetic linkage map for watermelon derived from recombinant inbred lines. J. Amer Soc Hortic Sci 129:237–243
Zhang H, Gong G, Guo S, Ren Y, Xu Y, Ling KS (2011) Screening the USDA watermelon germplasm collection for drought tolerance at the seedling stage. HortScience 46:1245–1248
Zhang H, Wang H, Guo S, Ren Y, Gong G, Weng Y, Xu Y (2012) Identification and validation of a core set of microsatellite markers for genetic diversity analysis in watermelon, Citrullus lanatus (Thunb.) Matsum. & Nakai. Euphytica 186:329–342
Zhang H, Fan J, Guo S, Ren Y, Gong G, Zhang J (2016) Genetic diversity, population structure, and formation of a core collection of 1197 citrullus accessions. HortScience 51:23–29
Zhang Z, Zhang Y, Sun L, Qiu G, Sun Y, Zhu Z, Luan F, Wang X (2018) Construction of a genetic map for Citrullus lanatus based on CAPS markers and mapping of three qualitative traits. Sci Hortic 233:532–538
Zhou M, Guo S, Zhang J, Zhang H, Li C, Tang X, Ren Y, Gong G (2016) Comparative dynamics of ethylene production and expression of the ACS and ACO genes in normal-ripening and non-ripening watermelon fruits. Acta Physiol Plant 38:228–241
Zhu H, Song P, Koo DH, Guo L, Li Y, Sun S, Weng Y, Yang L (2016) Genome wide characterization of simple sequence repeats in watermelon genome and their application in comparative mapping and genetic diversity analysis. BMC Genom 17:557
Zhu Q, Gao P, Liu S, Zhu Z, Amanullah S, Davis AR, Luan F (2017) Comparative transcriptome analysis of two contrasting watermelon genotypes during fruit development and ripening. BMC Genom 18:3
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The Limpopo Department of Agriculture and Rural Development (South Africa), and the University of KwaZulu-Natal (UKZN) are acknowledged for financial support of this study.
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Ngwepe, R.M., Mashilo, J. & Shimelis, H. Progress in genetic improvement of citron watermelon (Citrullus lanatus var. citroides): a review. Genet Resour Crop Evol 66, 735–758 (2019). https://doi.org/10.1007/s10722-018-0724-4
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DOI: https://doi.org/10.1007/s10722-018-0724-4