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Genetic Resources and Crop Evolution

, Volume 65, Issue 6, pp 1615–1624 | Cite as

Exploring genetic diversity and disease response of cultivated rice accessions (Oryza spp.) against Pyricularia oryzae under rainfed upland conditions in Benin

  • Octaviano Igor Yelome
  • Kris Audenaert
  • Sofie Landschoot
  • Alexandre Dansi
  • Wouter Vanhove
  • Drissa Silue
  • Patrick Van Damme
  • Geert Haesaert
Research Article

Abstract

The main goal of this study is to gain insight into the relationship between the genetic profile of cultivated rice (Oryza spp.) accessions and their resistance to rice blast. Therefore, the genetic and phenotypic variability of a set of 350 cultivated rice accessions originating from Africa (Benin, Mali and Nigeria, Ivory Coast etc.) was examined. Seventy-seven fluorescent amplified fragment polymorphism (AFLP) markers were used to gain insight into the genetic variation and to classify the germplasm collection. In addition, the rice germplasm was assessed for its resistance to blast disease caused by Pyricularia oryzae in upland field conditions. Huge differences in responses of rice accessions to P. oryzae were observed, ranging from highly susceptible to highly resistant. Twelve percent of all accessions were highly resistant to P. oryzae. Based on their AFLP marker profile these highly resistant accessions could be separated from the other accessions. Stepwise regression revealed that the best prediction of the blast resistance level was achieved with a maximum number of 13 AFLP markers. Marker CTA22 was the most important for accurate prediction of blast resistance, this marker was present in all highly resistant accessions. It can be concluded that AFLP markers are a valuable tool to screen rice accessions for their susceptibility towards blast disease and that, based on a subset of markers, it is possible to predict the resistance to rice blast.

Keywords

Fluorescent-AFLP Genetic diversity Rice Blast 

Notes

Acknowledgements

Funding of this Research work by the Monsanto’s Beachell-Borlaug International Scholars Program (MBBIS) and the Flemish Fund for Scientific Research (BOF) with support of AfricaRice. Special thanks to the AfricaRice genebank for providing seed and the related information.

Authors contribution

OINY carried out the field works, the genotyping, data analysis and drafted the manuscript. KA participated in project design, genotyping, data analysis and revised the manuscript. SL participated in statistical analysis of data and revised the manuscript. DS, AD, WV, PVD, and GH participated in project design, data analysis and revised the manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

10722_2018_638_MOESM1_ESM.docx (39 kb)
Supplementary material 1 (DOCX 39 kb)

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Copyright information

© Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  • Octaviano Igor Yelome
    • 1
  • Kris Audenaert
    • 1
  • Sofie Landschoot
    • 1
    • 2
  • Alexandre Dansi
    • 3
  • Wouter Vanhove
    • 1
  • Drissa Silue
    • 4
  • Patrick Van Damme
    • 1
    • 5
  • Geert Haesaert
    • 1
  1. 1.Department of Plants and Crops, Faculty of Bioscience EngineeringGhent UniversityGhentBelgium
  2. 2.Department of Data Analysis and Mathematical Modelling, Faculty of Bioscience EngineeringGhent UniversityGhentBelgium
  3. 3.Faculté des Sciences et Techniques de Dassa, Laboratoire de Biotechnologie, Ressources Génétiques et Amélioration des Espèces Animales et Végétales (BIORAVE)Université d’AbomeyCotonouBenin
  4. 4.AfricaRice CenterCotonouBenin
  5. 5.Faculty of Tropical AgriSciencesCzech University of Life Sciences PraguePrague 6, SuchdolCzech Republic

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