Genetic Resources and Crop Evolution

, Volume 63, Issue 4, pp 595–600 | Cite as

SSR assessment of the genetic diversity of emmer wheat with emphasis on Iranian landraces (Triticum dicoccon Schrank)

  • Mostafa Rafeipour
  • Ghader Mirzaghaderi
  • Salar Shaaf
  • Hedyeh Badakhshan
Short Communication


The cultivation of emmer wheat (Triticum dicoccon Schrank) in Iran has been decreased and limited to the marginal agricultural lands of western parts of the country. In the present study, 40 emmer wheat accessions including 13 Iranian landraces and 27 wild and domesticated accessions obtained from CIMMYT, were evaluated for genetic diversity using 24 SSR and four EST-SSR markers, distributed over all the chromosomes. The number of detected alleles was 168 (for all the studied accessions) and 84 (for the Iranian landraces), with average polymorphic information contents of 0.61 and 0.30, respectively. Based on the allelic information and cluster analysis, wild emmer wheats were more variable than domestic emmers and the Iranian emmer landraces had low genetic variability making an isolated cluster distinct from the domesticated emmers of Transcaucasia. The low genetic diversity in Iranian emmer landraces may be due to genetic drift and decreased cultivation area during the past years proving a need to manage emmer wheat genetic resources in Iran towards increasing its genetic diversity, breeding and conservation.


Genetic diversity Iran SSR marker Triticumdicoccon T.dicoccoides 


Complaince with ethical standards

Conflict of interest

The authors declare that they have no competing interests.


  1. Barcaccia G, Molinari L, Porfiri O, Veronesi F (2002) Molecular characterization of emmer (Triticum dicoccon Schrank) Italian landraces. Genet Resour Crop Evol 49:417–428CrossRefGoogle Scholar
  2. Botstein D, White RL, Skolnick M, Davis RW (1980) Construction of a genetic linkage map in man using restriction fragment length polymorphisms. Am J Hum Genet 32:314PubMedPubMedCentralGoogle Scholar
  3. Creste S, Neto AT, Figueira A (2001) Detection of single sequence repeat polymorphisms in denaturing polyacrylamide sequencing gels by silver staining. Plant Mol Biol Report 19:299–306CrossRefGoogle Scholar
  4. De Vita P, Riefolo C, Codianni P, Cattivelli L, Fares C (2006) Agronomic and qualitative traits of T. turgidum ssp. dicoccum genotypes cultivated in Italy. Euphytica 150:195–205CrossRefGoogle Scholar
  5. Doyle JJ, Doyle JL (1990) Isolation of plant DNA from fresh tissue. Focus 12:13–15Google Scholar
  6. Dvorak J, Zhang HB (1990) Variation in repeated nucleotide sequences sheds light on the phylogeny of the wheat B and G genomes. Proc Natl Acad Sci USA 87:9640–9644CrossRefPubMedPubMedCentralGoogle Scholar
  7. Fahima T, Röder M, Grama A, Nevo E (1998) Microsatellite DNA polymorphism divergence in Triticum dicoccoides accessions highly resistant to yellow rust. Theor Appl Genet 96:187–195CrossRefGoogle Scholar
  8. Figliuolo G, Perrino P (2004) Genetic diversity and intra-specific phylogeny of Triticum turgidum L. subsp. dicoccon (Schrank) Thell. revealed by RFLPs and SSRs. Genet Resour Crop Evol 51:519–527CrossRefGoogle Scholar
  9. Hammer Ø, Harper DAT, Ryan PD (2001) PAST: paleontological statistics software package for education and data analysis. Palaeontol Electron 4:9Google Scholar
  10. Huang X, Börner A, Röder M, Ganal M (2002) Assessing genetic diversity of wheat (Triticum aestivum L.) germplasm using microsatellite markers. Theor Appl Genet 105:699–707CrossRefPubMedGoogle Scholar
  11. Huson DH, Bryant D (2006) Application of phylogenetic networks in evolutionary studies. Mol Biol Evol 23:254–267CrossRefPubMedGoogle Scholar
  12. Lage J, Skovmand B, Peña RJ, Andersen SB (2006) Grain quality of emmer wheat derived synthetic hexaploid wheats. Genet Resour Crop Evol 53:955–962CrossRefGoogle Scholar
  13. Liu K, Muse SV (2005) PowerMarker: an integrated analysis environment for genetic marker analysis. Bioinformatics 21:2128–2129CrossRefPubMedGoogle Scholar
  14. Marcussen T, Sandve SR, Heier L, Spannagl M, Pfeifer M, Jakobsen KS, Wulff BB, Steuernagel B, Mayer KF, Olsen O-A (2014) Ancient hybridizations among the ancestral genomes of bread wheat. Science 345:1250092CrossRefPubMedGoogle Scholar
  15. Mondini L, Grausgruber H, Pagnotta MA (2014) Evaluation of European emmer wheat germplasm for agro-morphological, grain quality traits and molecular traits. Genet Resour Crop Evol 61:69–87CrossRefGoogle Scholar
  16. Mullan DJ, Platteter A, Teakle NL, Appels R, Colmer TD, Anderson JM, Francki MG (2005) EST-derived SSR markers from defined regions of the wheat genome to identify Lophopyrum elongatum specific loci. Genome 48:811–822CrossRefPubMedGoogle Scholar
  17. Pagnotta M, Mondini L, Atallah M (2005) Morphological and molecular characterization of Italian emmer wheat accessions. Euphytica 146:29–37CrossRefGoogle Scholar
  18. Pagnotta M, Mondini L, Codianni P, Fares C (2009) Agronomical, quality, and molecular characterization of twenty Italian emmer wheat (Triticum dicoccon) accessions. Genet Resour Crop Evol 56:299–310CrossRefGoogle Scholar
  19. Plaschke J, Ganal M, Röder M (1995) Detection of genetic diversity in closely related bread wheat using microsatellite markers. Theor Appl Genet 91:1001–1007PubMedGoogle Scholar
  20. Pujar S, Tamhankar SA, Rao VS, Gupta VS, Naik S, Ranjekar PK (1999) Arbitrarily primed-PCR based diversity assessment reflects hierarchical groupings of Indian tetraploid wheat genotypes. Theor Appl Genet 99:868–876CrossRefGoogle Scholar
  21. Röder MS, Korzun V, Wendehake K, Plaschke J, Tixier M-H, Leroy P, Ganal MW (1998) A microsatellite map of wheat. Genetics 149:2007–2023PubMedPubMedCentralGoogle Scholar
  22. Röder M, Wendehake K, Korzun V, Bredemeijer G, Laborie D, Bertrand L, Isaac P, Rendell S, Jackson J, Cooke R (2002) Construction and analysis of a microsatellite-based database of European wheat varieties. Theor Appl Genet 106:67–73PubMedGoogle Scholar
  23. Salamini F, Özkan H, Brandolini A, Schäfer-Pregl R, Martin W (2002) Genetics and geography of wild cereal domestication in the Near East. Nat Rev Genet 3:429–441PubMedGoogle Scholar
  24. Salunkhe A, Tamhankar S, Tetali S, Zaharieva M, Bonnett D, Trethowan R, Misra S (2013) Molecular genetic diversity analysis in emmer wheat (Triticum dicoccon Schrank) from India. Genet Resour Crop Evol 60:165–174CrossRefGoogle Scholar
  25. Song QJ, Shi JR, Singh S, Fickus EW, Costa JM, Lewis J, Gill BS, Ward R, Cregan PB (2005) Development and mapping of microsatellite (SSR) markers in wheat. Theor Appl Genet 110:550–560CrossRefPubMedGoogle Scholar
  26. Tautz D (1993) Notes on the definition and nomenclature of tandemly repetitive DNA sequences. In: Pena SDJ, Chakraborty R, Epplen JT, Jeffreys AJ (eds) DNA fingerprinting: state of the science. Birkhäuser Verlag, Basel, pp 21–28CrossRefGoogle Scholar
  27. Teklu Y, Hammer K (2006) Farmers’ perception and genetic erosion of tetraploid wheats landraces in Ethiopia. Genet Resour Crop Evol 53:1099–1113CrossRefGoogle Scholar
  28. Teklu Y, Hammer K, Huang XQ, Röder MS (2006) Analysis of microsatellite diversity in Ethiopian tetraploid wheat landraces. Genet Resour Crop Evol 53:1115–1126CrossRefGoogle Scholar
  29. Teklu Y, Hammer K, Röder M (2007) Simple sequence repeats marker polymorphism in emmer wheat (Triticum dicoccon; Schrank): analysis of genetic diversity and differentiation. Genet Resour Crop Evol 54:543–554CrossRefGoogle Scholar
  30. Willi Y, Van Buskirk J, Hoffmann AA (2006) Limits to the adaptive potential of small populations. Ann Rev Ecol Syst 37:433–458CrossRefGoogle Scholar
  31. Zaharieva M, Ayana N, Hakimi A, Misra S, Monneveux P (2010) Cultivated emmer wheat (Triticum dicoccon Schrank), an old crop with promising future: a review. Genet Resour Crop Evol 57:937–962CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2016

Authors and Affiliations

  1. 1.Agronomy and Plant Breeding Department, Faculty of AgricultureUniversity of KurdistanSanandajIran
  2. 2.Department of Agronomy and Plant Breeding, College of Agriculture and Natural Resources, Sanandaj BranchIslamic Azad UniversitySanandajIran

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