Genetic diversity assessment for Eugenia uniflora L., E. pyriformis Cambess., E. brasiliensis Lam. and E. francavilleana O. Berg neotropical tree species (Myrtaceae) with heterologous SSR markers
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In this study, a set of nuclear microsatellite markers were transferred and characterized from two Myrtaceae sub-families to four neotropical Eugenia fruit tree species (E. uniflora L., E. pyriformis Cambess., E. brasiliensis Lam. and E. francavilleana O. Berg), which are neglected and underutilized foods of great ecological and potential economic value found in the Atlantic forest regions of South America. Leaf samples of mature E. uniflora, E. pyriformis, E. brasiliensis and E. francavilleana trees, which are popularly termed “pitanga”, “uvaia”, “grumixama” and “guamirim”, respectively, were collected from two areas greatly impacted by the agricultural practices of the sugar cane industry in the state of São Paulo in the southeastern region of Brazil. A total of 19 simple sequence repeat (SSR) markers from the Myrtaceae sub-families Leptospermoideae and Myrtoideae were tested, and 15 polymorphic heterologous SSR markers were identified. The expected heterozygosities for E. uniflora, E. pyriformis, E. brasiliensis and E. francavilleana were 0.64, 0.75, 0.54 and 0.71, respectively. These results suggest that these SSR markers may be useful for population genetic studies. A total of 4, 9, 11 and 4 heterologous SSR polymorphic markers for E. uniflora, E. pyriformis, E. brasiliensis and E. francavilleana, respectively, are now available for genetic diversity, gene flow and mating system analyses in these species.
KeywordsEugenia uniflora E. pyriformis E. brasiliensis E. francavilleana Forest conservation Genetic diversity Heterologous SSR Neglected and underutilized fruit trees
This study was supported by Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP, Grant #03/04199-4 to ALAM), Provost for Research of São Paulo University (ALAM), Fundação de Apoio ao Ensino, Pesquisa e Assistência (FAEPA) (MAM) and CAPES-PROEX grants. RFR, KAGA, AR and MCG were supported by FAPESP (Grants 08/01559-3, 08/04121-9, 09/02714-5 and 07/04787-4, respectively). ALAM was supported by a research assistantship from FAPESP (2004/01378-8) and CNPq (PD Senior 150277/2009-1, PV 300140/2011-8). CAM is fellow CNPq researcher. The authors thank Antônio Justino da Silva (PCARP/USP), Marcelo Antonio de Pinho Ferreira and Vanderlei Aparecido Alves de Miranda (Laboratório de Ecologia e Restauração Florestal (LERF), Departamento de Ciências Biológicas da ESALQ/USP) for collaboration regarding sample collections.
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