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Do faba bean (Vicia faba L.) accessions from environments with contrasting seasonal moisture availabilities differ in stomatal characteristics and related traits?

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Abstract

Drought is a major constraint to faba bean (Vicia faba L.) production, and there are many mechanisms by which leaves can regulate water loss. Our primary objective was to test if the origin of the faba bean accessions, from drought-prone and non-drought-prone environments, was associated with differences in measurable aspects of stomatal morphology and physiology related to water use. Two sets, each consisting of 201 faba bean accessions, were chosen from environments with contrasting seasonal moisture profiles following the focused identification of germplasm strategy (FIGS), and then screened under well watered conditions. From these, two subsets of 10 accessions each were chosen to test for differences in response to drought. Parameters related to stomatal function and water status were measured. The dry-adapted set had bigger stomata, higher leaf relative water content (LRWC) and cooler leaves under well watered conditions. Stomatal density and stomatal area per unit area of leaflet were negatively correlated with gas exchange parameters and positively correlated with intrinsic water use efficiency. Drought caused stomatal densities to increase in the dry set while stomatal length decreased in both sets. The moisture deficit was sufficient to decrease gas exchange in both sets to similar levels, but the dry-adapted set maintained warmer leaves and a higher LRWC that showed no significant correlations with leaf morphology or gas exchange, demonstrating more effective stomatal regulation. The results also support that collection site data from the environment where genetic resources are collected can be used as indicators of adaptive traits in an herbaceous annual species.

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Abbreviations

Anet :

Photosynthetic rate

CTd:

Canopy temperature difference from air temperature

E:

Transpiration rate

FIGS:

Focused identification of germplasm strategy

gs :

Stomatal conductance

LRWC:

Leaf relative water content

LTd:

Leaflet temperature difference from air temperature

WU:

Water used

WUEb :

Biomass water use efficiency

WUEi :

Intrinsic water use efficiency

References

  • Abdelmula AA, Link W, von Kittlitz E, Stelling S (1999) Heterosis and inheritance of drought tolerance in faba bean, Vicia faba L. Plant Breed 118:845

    Google Scholar 

  • Abrams MD (1994) Genotypic and phenotypic variation as stress adaptation in temperate tree species: a review of several case studies. Tree Physiol 14:833–842

    Article  PubMed  Google Scholar 

  • Abrams MD, Kubiske ME, Steiner KC (1990) Drought adaptations and responses in five genotypes of Fraxinus pennsylvanica Marsh: photosynthesis, water relations and leaf morphology. Tree Physiol 6:305–315

    Article  PubMed  Google Scholar 

  • Amani I, Fischer RA, Reynolds MP (1996) Canopy temperature depression associated with yield of irrigated spring wheat cultivars in a hot climate. J Agron Crop Sci 176:119–129

    Article  Google Scholar 

  • Amede T, von Kittlitz E, Schubert S (1999) Differential drought responses of faba bean (Vicia faba L.) inbred lines. J Agron Crop Sci 183:35–45

    Article  Google Scholar 

  • Aminian A, Mohammadi S, Hoshmand S, Khodambashi M (2011) Chromosomal analysis of photosynthesis rate and stomatal conductance and their relationships with grain yield in wheat (Triticum aestivum L.) under water-stressed and well-watered conditions. Acta Physiol Plant 33:755–764

    Article  Google Scholar 

  • Barrs HD, Weatherley PE (1962) A re-examination of the relative turgidity technique for estimating water deficit in leaves. Aust J Biol Sci 15:413–428

    Google Scholar 

  • Blum A (2009) Effective use of water (EUW) and not water-use efficiency (WUE) is the target of crop yield improvement under drought stress. Field Crops Res 112:119–123

    Article  Google Scholar 

  • Blum A (2011) Plant breeding for water limited environments. Springer, New York

    Book  Google Scholar 

  • Blum A, Sullivan CY (1986) The comparative drought resistance of landraces of sorghum and millet from dry and humid regions. Ann Bot 57:835–846

    Article  Google Scholar 

  • Boyer JS (1982) Plant productivity and environment. Science 218:443–448

    Article  PubMed  CAS  Google Scholar 

  • Buttery BR, Tan CS, Buzzell RI, Gaynor JD, Mac Tavish DC (1993) Stomatal numbers of soybean and response to water stress. Plant Soil 149:283–288

    Article  Google Scholar 

  • Centritto M, Lauteri M, Monteverdi MC, Serraj R (2009) Leaf gas exchange, carbon isotope discrimination, and grain yield in contrasting rice genotype subjected to water deficits during the reproductive stage. J Exp Bot 60:2325–2339

    Article  PubMed  CAS  Google Scholar 

  • Darwish DS, Fahmy GM (1997) Transpiration decline curves and stomatal characteristics of faba bean genotypes. Biol Plant 39:243–249

    Article  Google Scholar 

  • Fischer RA, Rees D, Sayre KD, Lu ZM, Condon AG, Saavedra AL (1998) Wheat yield progress associated with higher stomatal conductance and photosynthetic rate, and cooler canopies. Crop Sci 38:1467–1475

    Article  Google Scholar 

  • Franks PJ, Drake PL, Beerling DJ (2009) Plasticity in maximum stomatal conductance considered by negative correlation between stomatal size and density: an analysis using Eucalyptus globulus. Plant Cell Environ 32:1737–1748

    Article  PubMed  Google Scholar 

  • Fraser LH, Greenall A, Carlyle C, Turkington R, Friedman CR (2009) Adaptive phenotypic plasticity of Pseudoroegneria spicata: response of stomatal density, leaf area and biomass to changes in water supply and increased temperature. Ann Bot 103:769–775

    Article  PubMed  Google Scholar 

  • Galmés J, Flexas J, Savé R, Medrano H (2007) Water relations and stomatal characteristics of Mediterranean plants with different growth forms and leaf habits: responses to water stress and recovery. Plant Soil 290:139–155

    Article  Google Scholar 

  • Grzesiak S, Iijima M, Kono Y, Yamauchi A (1997a) Differences in drought tolerance between cultivars of field bean and field pea. Morphological characteristics, germination and seedling growth. Acta Physiol Plant 19:339–348

    Article  Google Scholar 

  • Grzesiak S, Iijima M, Kono Y, Yamauchi A (1997b) Differences in drought tolerance between cultivars of field bean and field pea. A comparison of drought-resistance and drought-sensitive cultivars. Acta Physiol Plant 19:349–357

    Article  Google Scholar 

  • Hetherington AM, Woodward FI (2003) The role of stomata in sensing and driving environmental change. Nature 424:901–908

    Article  PubMed  CAS  Google Scholar 

  • Khan HR, Link W, Hocking TJH, Stoddard FL (2007) Evaluation of physiological traits for improving drought tolerance in faba bean (Vicia faba L.). Plant Soil 292:205–217

    Article  CAS  Google Scholar 

  • Khan HR, Paull JG, Siddique KHM, Stoddard FL (2010) Faba bean breeding for drought-affected environments: a physiological and agronomic perspective. Field Crops Res 115:279–286

    Article  Google Scholar 

  • Khazaei H, Monneveux P, Shao HB, Mohammady S (2010) Variation for stomatal characteristics and water use efficiency among diploid, tetraploid and hexaploid Iranian wheat landraces. Genet Resour Crop Evol 57:307–314

    Article  Google Scholar 

  • Leport L, Turner NC, French RJ, Tennat D, Thomson BD, Siddique KHM (1998) Water relations, gas exchange and growth of cool-season grain legumes in a Mediterranean-type environment. Eur J Agron 9:295–303

    Article  Google Scholar 

  • Mackay M, Street K (2004) Focused identification of germplasm strategy—FIGS. In: Black CK, Panozzo JF, Rebetzke GJ (eds) Proceedings of the 54th Australian Cereal Chemistry Conference and the 11th Wheat Breeders Assembly, 21st to 24th September 2004, Canberra, ACT, Australia. Cereal Chemistry Division, Royal Australian Chemical Institute (RACI), Melbourne, Victoria, Australia, pp 138–141

  • Malik TA, Ullah S, Malik S (2006) Genetic linkage of drought tolerant and agronomic traits in cotton. Pak J Bot 38:1613–1619

    Google Scholar 

  • Merah O, Monneveux P, Deléens E (2001) Relationship between flag leaf carbon isotope discrimination and several morpho-physiological traits in durum wheat genotypes under Mediterranean conditions. Environ Exp Bot 45:63–71

    Article  PubMed  Google Scholar 

  • Miskin KE, Rasmusson DC (1970) Frequency and distribution of stomata in barley. Crop Sci 10:575–578

    Article  Google Scholar 

  • Munns R, James RA, Sirault XRR, Furbank RT, Jones HG (2010) New phenotyping methods for screening wheat and barley for beneficial responses to water deficit. J Exp Bot 61:3499–3507

    Article  PubMed  CAS  Google Scholar 

  • Nerkar YS, Wilson D, Lawes DA (1981) Genetic variation in stomatal characteristics and behaviour, water use and growth of five Vicia faba L. genotypes under contrasting soil moisture regimes. Euphytica 30:335–345

    Article  Google Scholar 

  • Ohsumi A, Kanemura T, Homma K, Horie T, Shiraiwa T (2007) Genotypic variation of stomatal conductance to stomatal density and length in rice (Oryza sativa L.). Plant Prod Sci 10:322–328

    Article  CAS  Google Scholar 

  • Passioura JB, Angus JF (2010) Improving productivity of crops in water-limited environments. Adv Agron 106:37–75

    Article  Google Scholar 

  • Peleg Z, Fahima T, Abbo S, Krugman T, Nevo E, Yakir D, Saranga Y (2005) Genetic diversity for drought resistance in wild emmer wheat and its ecogeographical associations. Plant Cell Environ 28:176–191

    Article  Google Scholar 

  • Quarrie SA, Jones HG (1977) Effects of abscisic acid and water stress on development and morphology of wheat. J Exp Bot 28:192–203

    Article  CAS  Google Scholar 

  • R Development Core Team (2012) R: a language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. ISBN 3-900051-07-0, http://www.R-project.org

  • Ricciardi L (1989) Plant breeding for resistance to drought. II. Relationship between stomata and agronomic traits in Vicia faba L. genotypes. Agric Mediterr 119:424–434

    Google Scholar 

  • Sekiya M, Yano K (2008) Stomatal density of cowpea correlations with carbon isotope discrimination in different phosphorus, water and CO2 environment. New Phytol 179:799–807

    Article  PubMed  CAS  Google Scholar 

  • Spence RD, Wu H, Sharpe PJH, Clark KG (1986) Water stress effects on guard cell anatomy and the mechanical advantage of the epidermal cells. Plant Cell Environ 9:197–202

    Google Scholar 

  • St Paul NKM, Limousin JM, Rodriguez-Calcerrada J, Ruffault J, Rambal S, Letts MG, Misson L (2012) Photosynthetic sensitivity to drought varies among populations of Quercus ilex along a rainfall gradient. Funct Plant Biol 39:25–37

    Article  Google Scholar 

  • Suriyagoda LDB, Ryan MH, Renton M, Lambers H (2010) Multiple adaptive responses of Australian native perennial legumes with pasture potential to grow in phosphorus- and moisture-limited environments. Ann Bot 105:755–767

    Article  PubMed  CAS  Google Scholar 

  • Tanaka Y, Shiraiwa T, Nakajima A, Sato J, Nakazaki T (2008) Leaf gas exchange activities in soybean as related to leaf traits and stem growth habit. Crop Sci 48:1925–1932

    Article  CAS  Google Scholar 

  • Tanzarella OA, De Pace C, Filippetti A (1984) Stomatal frequency and size in Vicia faba L. Crop Sci 24:1070–1076

    Article  Google Scholar 

  • Wang H, Clarke JM (1993a) Genotypic, intraplant, and environmental variation in stomatal frequency and size in wheat. Can J Plant Sci 73:671–678

    Article  Google Scholar 

  • Wang H, Clarke JM (1993b) Relationship of excised-leaf water-loss and stomatal frequency in wheat. Can J Plant Sci 73:93–99

    Article  Google Scholar 

  • Xu Z, Zhou G (2008) Response of leaf stomatal density to water status and its relationship with photosynthesis in a grass. J Exp Bot 59:3317–3325

    Article  PubMed  CAS  Google Scholar 

  • Yang HM, Wang GX (2001) Leaf stomatal densities and distribution in Triticum aestivum under drought and CO2 enrichment. Chin J Plant Ecol 25:312–316

    Google Scholar 

  • Yang L, Han M, Zhou G, Li J (2007) The changes of water-use efficiency and stoma density of Leymus chinensis along Northeast China Transect. Acta Ecol Sin 27:16–24

    Article  Google Scholar 

  • Zhang YP, Wang ZM, Wu YC, Zhang X (2006) Stomatal characteristics of different green organs in wheat under different irrigation regimes. Acta Agron Sin 32:70–75

    CAS  Google Scholar 

Download references

Acknowledgments

H.K. expresses his gratitude to CIMO (Centre for International Mobility) and the Emil Aaltonen Foundation (Emil Aaltosen Säätiö) for their financial support. In addition, we would like to thank Markku Tykkyläinen and Sini Lindstrom, technical assistants of the glasshouse of Helsinki University and Guillermo Mínguez Vélaz, visiting student, for their kind assistance during the experiments.

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Authors and Affiliations

  1. Department of Agricultural Sciences, University of Helsinki, Latokartanonkaari 5, P.O. Box 27, 00014, Helsinki, Finland

    Hamid Khazaei, Arja Santanen & Frederick L. Stoddard

  2. International Centre for Agricultural Research in the Dry Areas (ICARDA), P.O. Box 5466, Aleppo, Syria

    Kenneth Street & Abdallah Bari

Authors
  1. Hamid Khazaei
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  2. Kenneth Street
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  3. Arja Santanen
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  4. Abdallah Bari
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  5. Frederick L. Stoddard
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Correspondence to Hamid Khazaei.

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Khazaei, H., Street, K., Santanen, A. et al. Do faba bean (Vicia faba L.) accessions from environments with contrasting seasonal moisture availabilities differ in stomatal characteristics and related traits?. Genet Resour Crop Evol 60, 2343–2357 (2013). https://doi.org/10.1007/s10722-013-0002-4

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  • DOI: https://doi.org/10.1007/s10722-013-0002-4

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