Genetic Resources and Crop Evolution

, Volume 59, Issue 8, pp 1597–1603 | Cite as

Diversity of culturable bacteria and occurrence of phytopathogenic species in bean seeds (Phaseolus vulgaris L.) preserved in a germplasm bank

  • Estefanía Trapiello
  • Ana J. González
Short Communication


The preservation of plant genetic resources involves the conservation of the microbial biota associated with them. The presence of culturable bacteria in a series of 16 bean seed batches, corresponding to nine local bean varieties, stored in a germplasm bank was studied by amplifying and sequencing the 16S rDNA. Microorganisms identified in seed lots were classified into three groups: environmental biota (present in all samples), biota characteristic of humans and animals (present in 53 % of samples) and phytopathogenic biota (present in 19 % of samples). Genus diversity ranged between 0.6931 and 2.0942 according to the Shannon–Weaver Index (H’), the sample presenting the highest number of plant pathogenic bacteria being the most diverse. This result suggests that contrary to common practice in diagnostic laboratories, it is necessary to identify all culturable bacteria isolates from each sample. In addition, the fact that potentially phytopathogenic bacteria have been preserved in a genebank should emphasize the importance of rigorous sanitary controls for plant genetic resources.


Biodiversity indices Curtobacterium flaccumfaciens Germplasm conservation Phaseolus vulgaris Plant pathogenic bacteria 



This work was supported by Project INIA-RF2007-00016-C04-03. The Spanish Plant Genetic Resource Center (CRF-INIA) has provided the batches of seeds for this study.


  1. Berger WH, Parker FL (1970) Diversity of planktonic foraminifera in deep sea sediments. Science 168:1345–1347PubMedCrossRefGoogle Scholar
  2. Chupp Ch, Sherf AF (1960) Vegetable diseases and their control. The Ronald Press Company, New YorkGoogle Scholar
  3. Edwards U, Rogall T, Blöcker H, Emde M, Böttger EC (1989) Isolation and direct complete nucleotide determination of entire genes. Characterization of a gene coding for 16S ribosomal RNA. Nucleic Acids Res 17:7843–7853PubMedCrossRefGoogle Scholar
  4. González AJ, Mendoza MC, Tello JC (2004) Microorganismos patógenos transmitidos por semilla de judía tipo granja asturiana. Saneamiento de semilla. SERIDA/KRK, Oviedo, SpainGoogle Scholar
  5. González AJ, Tello JC, Rodicio MR (2005) Bacterial wilt of beans (Phaseolus vulgaris) caused by Curtobacterium flaccumfaciens in the southeast of Spain. Plant Dis 89:1361CrossRefGoogle Scholar
  6. Margalef DR (1958) Information theory in ecology. Gen Syst 3:36–71Google Scholar
  7. Neergaard P (1988) Seed pathology, vol I and II. MacMillan, HoundmillsGoogle Scholar
  8. Shannon CE, Weaver W (1949) The mathematical theory of communication. University Illinois Press, UrbanaGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2012

Authors and Affiliations

  1. 1.Laboratorio de Fitopatología, Servicio Regional de Investigación y Desarrollo Agroalimentario (SERIDA)Villaviciosa, AsturiasSpain

Personalised recommendations