Skip to main content
SpringerLink
Log in

Molecular cloning and characterization of five novel low molecular weight glutenin subunit genes from Tibetan wheat landraces (Triticum aestivum L.)

  • Research Article
  • Published:
Genetic Resources and Crop Evolution Aims and scope Submit manuscript

Abstract

The low molecular weight glutenin subunits (LMW-GS) are the major components of glutenins and are important for the end-use quality of wheat. Five novel LMW-GS genes (designated as LMW-Jiachazharen, LMW-Bangdadongmai-3, LMW-Jiachaaigan, LMW-Maoyintumai and LMW-Rikezehongmai) were isolated from Tibetan wheat landraces. The coding regions of LMW-Jiachazharen, LMW-Bangdadongmai-3, LMW-Jiachaaigan, LMW-Maoyintumai and LMW-Rikezehongmai were 912, 897, 915, 927 and 906 bp in length, which encoded 302, 297, 303, 307 and 300 amino acid residues, respectively. Analysis of the deduced amino acid sequences showed that the five novel genes were classified as LMW-m type, with the predicted molecular weights of 32,013.97, 31,622.89, 32,107.07, 32,939.41 and 31,731.64 Da, respectively. The LMW-Jiachazharen, LMW-Jiachaaigan, LMW-Maoyintumai possessed seven cysteine residues, which resulted from a single-nucleotide polymorphism (SNP) of the G–A transition. However, except for eight conserved cysteine residues, LMW-Bangdadongmai-3 contained an extra one, as the result of a SNP of the T–C transition. In addition, the corresponding five LMW-GS were identified and confirmed by sodium SDS-PAGE and MALDI-TOF-MS, respectively. Phylogenetic analysis indicated that the five novel genes were glutenin-like proteins and designated as LMW-m type genes. The five novel genes may be new candidate LMW-GS genes with potential value for wheat quality improvement.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  • An X, Zhang Q, Yan Y, Li Q, Zhang Y, Wang A, Pei Y, Tian J, Wang H, Hsam SLK, Zeller FJ (2006) Cloning and molecular characterization of three novel LMW-i glutenin subunit genes from cultivated einkorn (Triticum monococcum L.). Theor Appl Genet 113:383–395

    Article  PubMed  CAS  Google Scholar 

  • Bietz JA, Shepherd KW, Wall JS (1975) Single kernel analysis of glutenin: use in wheat genetics and breeding. Cereal Chem 52:513–532

    CAS  Google Scholar 

  • Cassidy BG, Dvorak J, Anderson OD (1998) The wheat low-molecular-weight glutenin genes: characterization of six new genes and progress in understanding gene family structure. Theor Appl Genet 96:743–750

    Article  CAS  Google Scholar 

  • Chen F, Liu S, Zhao F, Xu C, Xia G (2010) Molecular characterisation of the low-molecular weight glutenin subunit genes of tall wheatgrass and functional properties of one clone Ee34. Amino Acids 38:991–999

    Article  PubMed  CAS  Google Scholar 

  • D’Ovidio R, Masci S (2004) The low-molecular-weight glutenin subunits of wheat gluten. J Cereal Sci 39:321–339

    Article  Google Scholar 

  • Feng B, An X, Xu Z, Liu D, Zhang A, Wu N, Wang T (2011) Molecular cloning of a novel chimeric HMW glutenin subunit gene 1Dx5′ from a common wheat line W958. Mol Breed 28:163–170

    Article  CAS  Google Scholar 

  • Gupta RB, Shepherd KW (1990) Two-step one-dimensional SDS-PAGE analysis of LMW subunits of glutenin. Theor Appl Genet 80:65–74

    CAS  Google Scholar 

  • Ikeda TM, Nagamine T, Fukupka H, Yano H (2002) Identification of new low-molecular-weight glutenin subunit genes in wheat. Theor Appl Genet 104:680–687

    Article  PubMed  CAS  Google Scholar 

  • Ikeda TM, Araki E, Fujita Y, Yano H (2006) Characterization of low-molecular-weight glutenin subunit genes and their protein products in common wheats. Theor Appl Genet 112:327–334

    Article  PubMed  CAS  Google Scholar 

  • Jackson EA, Holt LM, Payne PI (1983) Characterization of high-molecular-weight gliadin and low-molecular-weight subunits of wheat endosperm by two-dimensional electrophoresis and the chromosomal location of their controlling genes. Theor Appl Genet 66:29–37

    CAS  Google Scholar 

  • Jiang C, Pei Y, Zhang Y, Li X, Yao D, Yan Y, Ma W, Hsam SL, Zeller FJ (2008) Molecular cloning and characterization of four novel LMW glutenin subunit genes from Aegilops longissima, Triticum dicoccoides and T. zhukovskyi. Hereditas 145:92–98

    Article  PubMed  Google Scholar 

  • Lauriere M, Bouchez I, Doyen C, Eynard L (1996) Identification of glycosylated forms of wheat storage proteins using two-dimensional electrophoresis and blotting. Electrophoresis 17:497–501

    Article  PubMed  CAS  Google Scholar 

  • Lew EJL, Kuzmicky DD, Kasarda DD (1992) Characterization of low- molecular-weight glutenin subunits by reversed-phase high performance liquid chromatography, sodium dodecyl sulfate polyacrylamide gel electrophoresis, and N-terminal amino-acid sequencing. Cereal Chem 69:508–515

    CAS  Google Scholar 

  • Masci S, D’Ovidio R, Lafiandra D, Kasarda DD (1998) Characterization of a low-molecular-weight glutenin subunit gene from bread wheat and the corresponding protein that represents a major subunit of the glutenin polymer. Plant Physiol 118:1147–1158

    Article  PubMed  CAS  Google Scholar 

  • Murray MG, Thompson WF (1980) Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res 8(19):4321–4325

    Article  PubMed  CAS  Google Scholar 

  • Payne PI, Law CN, Mudd EE (1980) Control by homoeologous group 1 chromosome of the highmolecular-weight subunits of glutenin, a major protein of wheat endosperm. Theor Appl Genet 58:113–120

    Google Scholar 

  • Payne PI, Holt LM, Reader SM, Miller TE (1987) Chromosomal location of genes coding for endosperm proteins of Hordeum chilense, determined by two-dimensional electrophoresis of wheat-H. chilense chromosome addition lines. Biochem Genet 25:53–85

    Article  PubMed  CAS  Google Scholar 

  • Pei YH, Wang AL, An XL, Li XH, Zhang YZ, Huang XQ, Yan YM (2006) Characterization and comparative analysis of three low molecular weight glutenin C-subunit genes isolated from Aegilops tauschii. Can J Plant Sci 87:273–280

    Article  Google Scholar 

  • Shewry PR, Halford NG, Tatham AS (1992) High molecular weight subunits of wheat glutenin. J Cereal Sci 15:105–120

    Article  CAS  Google Scholar 

  • Yan ZH, Dai SF, Liu DC, Wei YM, Zheng YL (2007) Allelic variation of high molecular weight glutenin subunits in the hexaploid wheat landraces of Tibet, China. Int J Agric Res 2(9):838–843

    Article  CAS  Google Scholar 

  • Zeng XQ, Wang YJ, Li WY, Wang CY, Liu XL, Ji WQ (2010) Comparison of the genetic diversity between Triticum aestivum ssp. tibetanum Shao and Tibetan wheat landraces (Triticum aestivum L.) by using intron-splice junction primers. Genet Resour Crop Evol 57:1141–1150

    Article  Google Scholar 

  • Zhao HX, Wang RJ, Guo AG (2004) Development of primers specific for LMW-GS genes located on chromosome 1D and molecular characterization of a gene from Glu-D3 complex locus in bread wheat. Hereditas 141:193–198

    Article  PubMed  Google Scholar 

Download references

Acknowledgments

This work was supported by grants from the National Key Project of Transgenic Biologic Varieties Breeding of China (Grant No. 2011ZX08009-003) and the Knowledge Innovation Program of the Chinese Academy of Sciences (Grant No. KSCX3-EW-N-02).

Author information

Authors and Affiliations

  1. Chengdu Institute of Biology, Chinese Academy of Sciences, P.O.Box 416, Chengdu, 610041, China

    Qiuxia Lan, Bo Feng, Zhibin Xu, Guojun Zhao & Tao Wang

  2. Graduate University of Chinese, Academy of Sciences, Yuquan Road, Beijing, 100039, China

    Qiuxia Lan & Guojun Zhao

Authors
  1. Qiuxia Lan
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Bo Feng
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Zhibin Xu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Guojun Zhao
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Tao Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Tao Wang.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Lan, Q., Feng, B., Xu, Z. et al. Molecular cloning and characterization of five novel low molecular weight glutenin subunit genes from Tibetan wheat landraces (Triticum aestivum L.). Genet Resour Crop Evol 60, 799–806 (2013). https://doi.org/10.1007/s10722-012-9877-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10722-012-9877-8

Keywords

Search

Navigation