Abstract
The Sicilian grape cultivar ‘Nero d’Avola’ is among the oldest and most cultivated in the island, taking part in the production of several red wines exported worldwide, including DOC wines (Etna Rosso and Cerasuolo di Vittoria). Due to the ancient origin and repeated clonally propagation of the cultivar, phenotypic variability has been observed. Clone identification in this important cultivar has so far relied on phenotypic and chemical traits analyses, often affected by environmental conditions. Genetic markers, such as microsatellites, are particularly useful for cultivar identification, parentage testing, pedigree reconstruction and population structure studies. In the present paper, microsatellites were used to analyze the intra-varietal genetic diversity among 118 plants of ‘Nero d’Avola’, collected in 30 vineyards displaced in different areas of Sicily. Out of 22 microsatellites, 11 showed polymorphism among samples and 15 different phylogenetic groups were identified. Results show that ‘Nero d’Avola’ actually comprises different genetic profiles, although most of clones share a common origin.
References
Brookfield JFY (1996) A simple new method for estimating null allele frequency from heterozygote deficiency. Mol Ecol 5:453–455
Carimi F, Mercati F, Abbate L, Sunseri F (2010) Microsatellite analyses for evaluation of genetic diversity among Sicilian grapevine cultivars. Genet Resour Crop Evol 57:703–719
Costantini L (1989) Plant exploitation at Grotta dell’Uzzo, Sicily: new evidence for the transition from Mesolithic to Neolithic subsistence in southern Europe. In: Harris DR, Hillman GC (eds) Foraging and farming: the evolution of the plant exploitation. Unwin & Hyman, London, pp 197–206
Costantini L, Monaco A, Vouillamoz JF, Forlani M, Grando MS (2005) Genetic relationships among local Vitis vinifera cultivars from Campania. Vitis 44:25–34
Cupani F (1696) Hortus Catholicus seù illustrissimi, and excellentissimi principis Catholicae. Ed Benzi, Neapoli
Di Rosa C, Gringeri F (1966) Nero d’Avola, il vitigno dei più prestigiosi vini di Sicilia. Associazione Turistica Pro-Loco di Avola, AAPIT, Siracusa
Doyle JJ, Doyle LJ (1990) Isolation of plant DNA from fresh tissue. Focus 12:13–15
Geremia G (1839) Vertunno Etneo ovvero Stafulegrafia, storia delle varietà delle uve che trovasi nel d’intorno dell’Etna. Atti dell’Accademia Gioenia, Catania 14:3–55 and 57–68
González-Techera A, Jubany S, Ponce de Leon I, Boido E, Dellacasa E, Carrau FM (2004) Molecular diversity within clones of cv. Tannat (Vitis vinifera L.). Vitis 43:179–185
Gryta H, Carriconde F, Charcosset JY, Jargeat P, Gardes M (2005) Population dynamics of the ectomycorrhizal fungal species Tricholoma populinum and Tricholoma scalpturatum associated with black poplar under differing environmental conditions. Environ Microbiol 8:773–786
Moncada X, Hinrichsen P (2007) Limited genetic diversity among clones of red wine cultivar “Carmenère” as revealed by microsatellite an AFLP markers. Vitis 46:174–181
Moncada X, Munoz L, Castro M, Hinrichsen P, Merdinoglu D (2005) Clonal polymorphism in the red wine cultivars ‘Carmenere’ and ‘Cabernet Sauvignon’. Acta Hortic 689:513–519
Moncada X, Pelsy F, Merdinoglu D, Hinrichsen P (2006) Genetic diversity and geographical dispersal in grapevine clones revealed by microsatellite markers. Genome 49:1459–1472
Paetkau D, Calvert W, Stirling I, Stroberk C (1995) Microsatellite analysis of population structure in Canadian polar bears. Mol Ecol 4:347–354
Peakall R, Smouse PE (2006) GenAlEx 6: genetic analysis in excel. Population genetic software for teaching and research. Mol Ecol Notes 6:288–295
Regner F, Wiedeck E, Stadlbauer A (2000) Differentiation and identification of white Riesling clones by genetic markers. Vitis 39:103–107
Regner F, Hack R, Santiago JL (2006) Highly variable Vitis microsatellite loci for the identification of Pinot Noir clones. Vitis 45:85–91
Rohlf FJ (1998) Numerical taxonomy and multivariate analysis system ver. 2.02. Applied Biostatics Inc., New York
Sefc KM, Lopes MS, Lefort F, Botta R, Roubelakis-Angelakis KA, Ibanez J, Pejic I, Wagner HW, Glossl J, Steinkellner H (2000) Microsatellite variability in grapevine cultivars from different European regions and evaluation of assignment testing to assess the geographic origin of cultivars. Theor Appl Genet 100:498–505
Siegfried LK (2000) Accurate gene diversity estimates from amplified fragment length polymorphism (AFLP) markers. Mol Ecol 9:1241–1245
Tessier C, David J, Thip P, Boursiquot JM, Charrier A (1999) Optimization of the choice of molecular markers for varietal identification in Vitis vinifera L. Theor Appl Genet 98:171–177
Wagner HW, Sefc KM (1999) Identity 1.0. Centre for Applied Genetics, University of Agricultural Sciences
Weber JL (1990) Informativeness of human (dC-dA)n-(dGdT)n polymorphisms. Genomics 7:524–530
Yeh FC, Yang RC, Boyle T (1999) Popgene, version 1.31. Centre for International Forestry Research and University of Alberta, Edmonton
Acknowledgments
This research was partly supported by a grant from “Assessorato Agricoltura e Foreste, Regione Sicilia” in the frame of the project “Caratterizzazione tecnologica e genetica di microrganismi autoctoni ed interazione con i migliori cloni dei vitigni ‘Nero d’Avola’ ed Inzolia per migliorare alcune produzioni vitivinicole tipiche della Regione Sicilia” (Project Leader Prof. Patrizia Romano—Università degli Studi della Basilicata) and by the grant from the Italian Ministry of the Research in the frame of the project ‘Tracciabilità della filiera vitivinicola’ (OR 2.1.2—CISIA DGLS 191/2009).
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Carimi, F., Mercati, F., De Michele, R. et al. Intra-varietal genetic diversity of the grapevine (Vitis vinifera L.) cultivar ‘Nero d’Avola’ as revealed by microsatellite markers. Genet Resour Crop Evol 58, 967–975 (2011). https://doi.org/10.1007/s10722-011-9731-4
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DOI: https://doi.org/10.1007/s10722-011-9731-4