Intra-varietal genetic diversity of the grapevine (Vitis vinifera L.) cultivar ‘Nero d’Avola’ as revealed by microsatellite markers
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The Sicilian grape cultivar ‘Nero d’Avola’ is among the oldest and most cultivated in the island, taking part in the production of several red wines exported worldwide, including DOC wines (Etna Rosso and Cerasuolo di Vittoria). Due to the ancient origin and repeated clonally propagation of the cultivar, phenotypic variability has been observed. Clone identification in this important cultivar has so far relied on phenotypic and chemical traits analyses, often affected by environmental conditions. Genetic markers, such as microsatellites, are particularly useful for cultivar identification, parentage testing, pedigree reconstruction and population structure studies. In the present paper, microsatellites were used to analyze the intra-varietal genetic diversity among 118 plants of ‘Nero d’Avola’, collected in 30 vineyards displaced in different areas of Sicily. Out of 22 microsatellites, 11 showed polymorphism among samples and 15 different phylogenetic groups were identified. Results show that ‘Nero d’Avola’ actually comprises different genetic profiles, although most of clones share a common origin.
KeywordsClonal identification Simple sequence repeat Synonymies and homonyms Vitis vinifera L.
This research was partly supported by a grant from “Assessorato Agricoltura e Foreste, Regione Sicilia” in the frame of the project “Caratterizzazione tecnologica e genetica di microrganismi autoctoni ed interazione con i migliori cloni dei vitigni ‘Nero d’Avola’ ed Inzolia per migliorare alcune produzioni vitivinicole tipiche della Regione Sicilia” (Project Leader Prof. Patrizia Romano—Università degli Studi della Basilicata) and by the grant from the Italian Ministry of the Research in the frame of the project ‘Tracciabilità della filiera vitivinicola’ (OR 2.1.2—CISIA DGLS 191/2009).
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