Microsatellite mapping of genes for semi-dwarfism and branched spike in Triticum durum Desf. var. ramosoobscurum Jakubz. “Vetvistokoloskaya”
- 272 Downloads
A combination of increased harvest index and increased seed number per plant may improve wheat yield. We found an accession Triticum durum Desf. var. ramosoobscurum Jakubz. “Vetvistokoloskaya” R-107, whose plant height was short and spike was branched. To characterize R-107, we mapped the genes for semi-dwarfism and branched spike. Semi-dwarf gene Rht_R107 (Reduced height R107) from R-107 was allelic to Rht11 and Rht-B1b on chromosome 4BS. Rht_R107, as well as Rht11, was recessive and insensitive to gibberellic acid. Microsatellite mapping indicated that Rht_R107 was linked to Xwmc48 (1.2 cM) on chromosome 4BS, and Rht11 was also linked to Xgwm495 marker (19.4 cM) on chromosome 4BS. The locus responsible for branched spike in R-107 was mapped by genotyping three F2 populations using microsatellite markers. Phenotypic analysis revealed that branched spike was under control of a recessive allele at a single locus. The bh (branched head) locus was located on chromosome 2AS and the marker Xgwm425 flanked the bh gene proximally.
KeywordsBranched spike Genetic resource GA3-insensitive Plant height Semi-dwarfism Triticum durum
We acknowledge the gift of seed of semi-dwarf accessions from the National Small Grain Collection (NGSC), Aberdeen, Idaho, USA and the Leibniz Institute of Plant Genetics and Crop Research (IPK) in Gatersleben, Germany. We thank Dr. D. L. Klindworth, USDA-ARS, Northern Crop Science Lab., Fargo, USA for helpful comments on our manuscript. M.A.H. deeply appreciates the Scholarship provided by the Ministry of Education, Science, Culture and Sports, Japanese Government, the unknown tax payers in Japan, and M.P. deeply appreciates the financial support from the Ministry of Education, Youth and Sports of the Czech Republic, project KONTAKT—ME10063.
- Evans LT (1993) Crop evolution, adaptation and yield. Cambridge University Press, Cambridge, p 500Google Scholar
- Martinek P, Bednář J (2001) Changes of spike morphology (multirow spike-MRS, long glumes-LG) in wheat (Triticum aestivum L.) and their importance for breeding. In: The proceedings of international conference “genetic collections, isogenic and alloplasmic lines”, Novosibirsk, Russia, pp 192–194Google Scholar
- Perry MW, D’Antuono MF (1989) Yield improvement and associated characteristics of some Australian spring wheat cultivars introduced between 1860 and 1982. Aust J Agric Res 40:457–472Google Scholar
- Sears ER (1954) The aneuploids of common wheat. University of Missouri, Columbia, pp 3–58Google Scholar
- Swaminathan MS, Chopra VL, Sastry GRK (1966) Expression and stability of an induced mutation for ear branching in bread wheat. Curr Sci 35:91–92Google Scholar
- Watanabe N (2004) Triticum polonicum IC12196: a possible alternative source of GA3-insensitive semi-dwarfism. Cereal Res Commun 32:429–434Google Scholar