Genetic Resources and Crop Evolution

, Volume 57, Issue 7, pp 1091–1107 | Cite as

Genetic characterization and species relationships among selected Asiatic Vigna Savi

  • Ruchi Vir
  • Tabassum Jehan
  • K. V. Bhat
  • S. Lakhanpaul
Research Article


Twenty-four ISSR primers were employed for diversity analysis, genotyping and assessment of species relationships in Asiatic Vigna from the Indian subcontinent. A total of 396 fragments were scored with an average of 16.5 products per primer. Primer no 827 was the most informative primer with highest resolving power (Rp), marker index (MI) as well as genotypic index (GI). Linear correlation was observed between Rp, MI and GI. The wild forms, V. mungo var. silvestris, V. radiata var. sublobata and V. radiata var. setulosa grouped closer to their respective cultigens. V. hainiana emerged as a distinct taxon and seems to be more primitive in comparison to the other close wild relatives of greengram and blackgram. Moderate gene flow and genetic differentiation was detected between the species analyzed. Analysis of molecular variance performed for the group (mungoradiatahainiana) in three different combinations revealed that nearly half of the variation exists within the populations while the remaining half is equally distributed among species and among populations within species.


Asiatic Vigna Genotypic index ISSR Marker index Resolving power 



RV gratefully acknowledges the financial support from the Council of Scientific and Industrial Research, Government of India. Project Director, National Research Centre on DNA Fingerprinting and Director, National Bureau of Plant Genetic Resources, New Delhi are gratefully acknowledged for the encouragement and consent to undertake this work.


  1. Ajibade SR, Weeden NF, Chite SM (2000) Inter simple sequence repeat analysis of genetic relationships in the genus Vigna. Euphytica 111:47–55CrossRefGoogle Scholar
  2. Babu CR, Sharma SK, Johri BM (1985) Taxonomic revision of Indian Phaseolus, Vigna, Macroptilium and Dysolobium. Bull Bot Surv Ind 27:1–28Google Scholar
  3. Blair MW, Panaud O, McCouch SR (1999) Inter simple sequence repeat (ISSR) amplification for analysis of micro satellite motif frequency and fingerprinting in rice (Oryza sativa L.). Theor Appl Genet 98:780–792CrossRefGoogle Scholar
  4. Bornet B, Branchard M (2001) Non anchored inter simple sequence repeat (ISSR) markers: reproducible and specific tools for genome fingerprinting. Plant Mol Biol Report 19:209–215CrossRefGoogle Scholar
  5. Brunk CF, Jones KC, James TW (1979) Assay for nanogram quantities of DNA in cellular homogenates. Anal Biochem 92:497–500CrossRefPubMedGoogle Scholar
  6. Charters YM, Robertson A, Wilkinson MJ, Ramsay G (1996) PCR analysis of oilseed rape cultivars (Brassica napus L. oleifera) using 5’-anchored simple sequence repeat (SSR) primers. Theor Appl Genet 92:442–447CrossRefGoogle Scholar
  7. Cosner ME, Crawford DJ (1994) Comparison of isozyme diversity in three rare species of Coreopsis (Asteraceae). Syst Bot 19:350–358CrossRefGoogle Scholar
  8. Doi K, Kaga A, Tomooka N, Vaughan DA (2002) Molecular phylogeny of genus Vigna subgenus Ceratotropis based on rDNA ITS and atpB-rbcL intergenic spacer for cpDNA sequences. Genetica 114:129–145CrossRefPubMedGoogle Scholar
  9. Excoffier L, Smouse PE, Quattro JM (1992) Analysis of molecular variance inferred from metric distances among DNA haplotypes: applications to human mitochondrial DNA restriction sites. Genetics 131:479–491PubMedGoogle Scholar
  10. Fang DQ, Rose ML, Krueger RR, Federica CT (1997) Fingerprinting trifoliate orange germplasm accessions with isozymes, sraps, and inter simple sequence repeats markers. Theor Appl Genet 95:211–219CrossRefGoogle Scholar
  11. Fatokun CA, Danish D, Young ND (1993a) Molecular taxonomic relationships in the genus Vigna based on RFLP analysis. Theor Appl Genet 86:97–104CrossRefGoogle Scholar
  12. Fatokun CA, Danish D, Young ND, Stewart EL (1993b) Molecular taxonomic relationships in subgenus Ceratotropis (genus Vigna) as revealed by RAPD analysis. Euphytica 88:17–24Google Scholar
  13. Gilbert JE, Lewis RV, Wilkinson MJ, Calgary PDS (1999) Developing an appropriate strategy to assess genetic variability in plant germplasm collections. Theor Appl Genet 98:1125–1131CrossRefGoogle Scholar
  14. Gomez-Campo C (1980) Morphology and morphotaxonomy of the tribe brassiere. In: Sundaes P, Gomez-Campo C (eds) Brassica crops and wild allies. Japan scientific societies press, Tokyo, pp 3–31Google Scholar
  15. Gomez-Campo C (1999) Taxonomy. In: Gomez-Campo C (ed) Biology of Brassica cenospecies. Elsevier, Amsterdam, pp 3–23CrossRefGoogle Scholar
  16. Gopinathan MC (1984) Population biological studies in Vigna minima (Roxb.) Ohwi et Ohashi and its allies. Ph D. thesis, Delhi University, Delhi, IndiaGoogle Scholar
  17. Gopinathan MC, Babu CR (1986) Meiotic studies of F1 hybrids between rice bean (Vigna umbellata) and its wild relative (V. minima). Genetica 71:115–117CrossRefGoogle Scholar
  18. Gopinathan MC, Babu CR, Shivanna KR (1986) Interspecific hybridization between rice bean (Vigna umbellata) and its wild relative (V. minima): fertility-sterility relationships. Euphytica 35:1017–1022CrossRefGoogle Scholar
  19. Hamrick JL, Godt MJ (1990) Allozyme diversity in plant species. In: Brown AHD, Clegg MT, Kahler AL, Weir BS (eds) Plant population genetics, breeding and germplasm resources. Sinauer, Sunderland, pp 43–63Google Scholar
  20. Iruela M, Pubio J, Cubero JI, Gil J, Millan T (2002) Phylogenetic analysis in the genus Cicer and cultivated chickpea using RAPD and ISSR markers. Theor Appl Genet 103:643–651CrossRefGoogle Scholar
  21. Jaaska V, Jaaska V (1990) Isozyme variation in Asian beans. Bot Acta 103:281–290Google Scholar
  22. Jaccard P (1908) Etude comparative de a distribution florale dans portiondes Alpes et des Jura. Bull Soc Vaudoise Sci Nat 37:547–579Google Scholar
  23. Joshi SP, Gupta VS, Aggarwal RK, Ranjekar PK, Brar DS (2000) Genetic diversity and phylogenetic relationship as revealed by inter simple sequence repeat (ISSR) polymorphism in the genus Oryza. Theor Appl Genet 100:1311–1320CrossRefGoogle Scholar
  24. Kaga A, Tomooka N, Egawa Y, Hosaka K, Kamijima O (1996) Species relationships in the subgenus Ceratotropis (genus Vigna) as revealed by RAPD analysis. Euphytica 88:17–24CrossRefGoogle Scholar
  25. Kajiwara H, Tomooka N (1998) Comparative analysis of genus Vigna seeds using antiserum against synthesized multiple antigenic peptide. Electrophoresis 19:3110–3113CrossRefPubMedGoogle Scholar
  26. Kocheiva EZ, Ryzhova NN, Khrapalova IA, Pukhalskyi VA (2002) Genetic diversity and phylogenetic relationships in the genus Lycopersicon (Tourn.) Mill. as revealed by inter simple sequence repeat (ISSR) analysis. Russ J Genet 38:958–966CrossRefGoogle Scholar
  27. Lewontin R (1972) The apportionment of human diversity. Evol Biol 6:381–398Google Scholar
  28. Malik M, Vyas P, Rangaswamy NS, Shivanna KR (1999) Development of two new cytoplasmic male sterile lines in Brassica juncea through wide hybridization. Plant Breed 118:75–78CrossRefGoogle Scholar
  29. Maréchal R, Mascherpa JM, Stainier F (1978) Etude taxonomique d’ un group complexe d’ espèces des genres Phaseolus et Vigna (Papilionaceae) sur la base de données morphologiques, et polliniques traitées par l’ analyse informatique. Boissiera 28:1–273Google Scholar
  30. McDermott JM, McDonald BA (1993) Gene flow in plant pathosystems. Annu Rev Phytopathol 31:353–373CrossRefGoogle Scholar
  31. McGregor CE, Lambert CE, Greyling MM, Louw JH, Warnich L (2000) A comparative assessment of DNA fingerprinting techniques (RAPD, ISSR, AFLP and SSR) in tetraploid potato (Solanum tuberosum L.) germplasm. Euphytica 113:135–144CrossRefGoogle Scholar
  32. Mohammadi SA, Prasanna BM (2003) Analysis of genetic diversity in crop plants- salient statistical tools and considerations. Crop Sci 43:1235–1248CrossRefGoogle Scholar
  33. Moreno S, Martin JB, Ortiz JM (1998) Inter simple sequence repeats PCR for characterization of closely related grapevine germplasm. Euphytica 101:117–125CrossRefGoogle Scholar
  34. Nei M (1972) Genetic distance between populations. Am Nat 106:283–292CrossRefGoogle Scholar
  35. Nei M (1987) Molecular evolutionary genetics. Columbia University Press, New YorkGoogle Scholar
  36. Pavliczek A, Hrda S, Flegr J (1999) Free-tree- freeware program for construction of phylogenetic trees on the basis of distance data and bootstrap/ jackknife analysis of the tree robustness. Application in the RAPD analysis of genus Frenkelia. Folia Biol 45:97–99Google Scholar
  37. Powell W, Machray GC, Provan J (1996) Polymorphism revealed by simple sequence repeats. Trends Plant Sci 7:216–222Google Scholar
  38. Prevost A, Wilkinson MJ (1999) A new system of comparing PCR primers applied to ISSR fingerprinting of potato cultivars. Theor Appl Genet 98:107–112CrossRefGoogle Scholar
  39. Rao GU, Batra-Sarup V, Prakash S, Shivanna KR (1994) Development of a new cytoplasmic male sterile lines in Brassica juncea through wide hybridization. Plant Breed 112:171–174CrossRefGoogle Scholar
  40. Reddy P, Sarla N, Siddiq EA (2002) Inter simple sequence repeat (ISSR) polymorphism and its application to plant breeding. Euphytica 128:9–17CrossRefGoogle Scholar
  41. Rohlf FJ (2002) NTSYS-pc numerical taxonomy and multivariate taxonomy system, version 2.11, Exeter software. Seteauket, New YorkGoogle Scholar
  42. Saini A, Jawali N (2009) Molecular evolution of 5S rDNA region in Vigna subgenus Ceratotropis and its phylogenetic implications. Plant Syst Evol 280:187–206CrossRefGoogle Scholar
  43. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring HarborGoogle Scholar
  44. Sudupak MA (2004) Inter and intra-species inter simple sequence repeat (ISSR) variations in the genus Cicer. Euphytica 135:229–238CrossRefGoogle Scholar
  45. Tateishi Y (1996) Systematics of the species of Vigna subgenus Ceratotropis. In: Srinives P, Kitbamroong C, Miyazaki S (eds) Mungbean germplasm: collection evaluation and utilization for breeding program. JIRCAS, Japan, pp 9–24Google Scholar
  46. Tateishi Y, Ohashi H (1990) Systematics of the Azuki bean in the genus Vigna. In: Fujii KA, Gatehouse MR, Johnson CD, Mitchel R, Yoshida T (eds) Bruchids and legumes: economics, ecology and coevolution. Kluwer, Dordrecht, pp 189–199Google Scholar
  47. Tomooka N, Lairungreang C, Egawa Y (1996) Taxonomic position of wild Vigna species collected in Thailand based on RAPD analysis. In: Srinives P, Kitbamroong C, Miyazaki S (eds) Mungbean germplasm: collection, evaluation and utilization for breeding program. JIRCAS, Tsukuba, pp 31–40Google Scholar
  48. Tomooka N, Yoon MS, Doi K, Kaga A, Vaughan D (2002) AFLP analysis of diploid species in the genus Vigna subgenus Ceratotropis. Genet Resour Crop Evol 49:521–530CrossRefGoogle Scholar
  49. Vaillancourt RE, Weeden NF (1993) Lack of isozyme similarity between Vigna unguiculata and other species of genus Vigna (Leguminosae). Can J Bot 71:586–591Google Scholar
  50. Verdcourt B (1970) Studies in the leguminosae-papilionoidae for the ‘Flora of Tropical East Africa’. Kew Bull 24:507–569CrossRefGoogle Scholar
  51. Yee E, Kidwell KK, Sill GR, Lumpkin TA (1999) Diversity among selected Vigna angularis (Azuki) accessions on the basis of RAPD and AFLP markers. Crop Sci 39:268–275CrossRefGoogle Scholar
  52. Yoon MS, Kaga A, Tomooka N, Vaughan DA (2000) Analysis of genetic diversity in the Vigna minima complex and related species in East Asia. J Plant Res 113:375–386CrossRefGoogle Scholar
  53. Ziekiewicz E, Rafalski A, Labuda D (1994) Genome fingerprinting by simple sequence repeat (SSR)- anchored polymerase chain reaction amplification. Genomics 20:176–183CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Ruchi Vir
    • 1
  • Tabassum Jehan
    • 1
  • K. V. Bhat
    • 2
  • S. Lakhanpaul
    • 1
  1. 1.Department of BotanyDelhi UniversityNew DelhiIndia
  2. 2.National Research Centre on DNA FingerprintingNational Bureau of Plant Genetic ResourcesNew DelhiIndia

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