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Genetic Resources and Crop Evolution

, Volume 57, Issue 3, pp 431–441 | Cite as

Cytological and RAPD data revealed genetic relationships among nine selected populations of the wild bramble species, Rubus parvifolius and R. coreanus (Rosaceae)

  • Xiao-rong Wang
  • Yuan Liu
  • Bi-feng Zhong
  • Xiao-li Dong
  • Qing Chen
  • Wu-feng Xia
  • Hanwu Zhang
  • Hao-ru Tang
Research Article

Abstract

Rubusparvifolius L. and R. coreanus Miq. are the two morphologically distinct, endemic wild brambles in East Asia that may be useful in raspberry breeding and cultivar development. In this study, genetic relationships among a total of nine populations of these two species, collected from Sichuan Province in southwestern China, were investigated by cytological and RAPD analyzing. With the exception of ploidy levels, R. parvifolius and R. coreanus had similar cytological features. Six R. parvifolius populations included diploids, triploid, tetraploid, and mixed diploid-tetraploid cytotype plants; among them, the population that produced the fewest seeds was the triploid, while the three R. coreanus populations were all diploid. But obvious genetic differentiation between R. parvifolius and R. coreanus was revealed by RAPD markers, the Euclidean’s GS coefficient generated by UPGMA cluster analysis between R. parvifolius and R. coreanus was only 0.442, about half of that observed within species. Both genetic relationships among these populations and the origin of polyploidy in R. parvifolius populations are also discussed.

Keywords

Genetic resources Karyotype Mixed cytotypes Morphological diversity Polyploidy Rubus coreanus Rubus parvifolius 

Notes

Acknowledgments

The authors are thankful to the programs of the National Natural Science Foundation of China (30671454) and the Education Bureau of Sichuan Province, China (2004A025), for financial support. We particularly thank Prof. Guang-hui Yang (Sichuan Agricultural University) for her help in the course of specimen identification.

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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Xiao-rong Wang
    • 1
  • Yuan Liu
    • 1
  • Bi-feng Zhong
    • 1
  • Xiao-li Dong
    • 1
  • Qing Chen
    • 1
  • Wu-feng Xia
    • 1
  • Hanwu Zhang
    • 1
  • Hao-ru Tang
    • 1
  1. 1.College of HorticultureSichuan Agricultural UniversityYa’an CityChina

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