Abstract
Couepia is a genus distributed in tropical regions of America. The nut of some Couepia species is used as fresh fruit and oil source for local communities. Despite its critical situation of conservation and its economic potential, there is a lack of information on the genetic variability of Couepia species. This study examines AFLP variation among 40 accessions of Couepia collected in the Colombian Amazonian region, representing two species: Couepia dolichopoda and Couepia subcordata. The individuals were examined for 96 markers generated from four EcoRI/MseI primer pairs, with 80% polymorphism across all accessions studied. According to cluster analysis, 40 accessions were grouped into two major clusters, corresponding to the two species analyzed, except one case whose situation is discussed. In C. dolichopoda accessions, significant correlation between the clustering pattern and the geographical origin was detected; the extent of variation within and among its collect sites was examined by AMOVA. The knowledge about the genetic variability of the accessions examined contributes to development of Couepia conservational efforts.
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Acknowledgements
We thank all the teams of general project “Conservación, Manejo y Aprovechamiento Sostenible de nueces promisorias pertenecientes al género Couepia por comunidades de veredas de los municipios Puerto Rico y Morelia, Caquetá”, funded by “Fondo para la Acción Ambiental”. Special thanks to the Molecular Biology Laboratory team of SINCHI institute.
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Rozo, Y., Quintero, L., Parra, M. et al. Analysis of genetic variability in Couepia accessions using AFLP markers. Genet Resour Crop Evol 56, 77–83 (2009). https://doi.org/10.1007/s10722-008-9346-6
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DOI: https://doi.org/10.1007/s10722-008-9346-6