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Characterization of fertile amphidiploid between Raphanus sativus and Brassica alboglabra and the crossability with Brassica species

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Abstract

A fertile amphidiploid × Brassicoraphanus (RRCC, 2n = 36) between Raphanus sativus cv. HQ-04 (2n = 18, RR) and Brassica alboglabra Bailey (2n = 18, CC) was synthesized and successive selections for seed fertility were made from F4 to F10. F10 plants exhibited good fertility with 14.9 seeds per siliqua and 32.3 g seeds per plant. Cytological observation revealed that frequent secondary pairing occurred among 3 chromosome pairs in pollen mother cells of plants (F4) with lower fertility, but not of plants with high fertility (F10). GISH analysis indicated that these F10 plants included the expected 18 chromosomes from R. sativus and B. alboglabra, respectively, but they lost approximately 27.6% R. sativus and 35.6% B. alboglabra AFLP (amplified fragment length polymorphism) bands. The crossability of the Raphanobrassica with R. sativus and 5 Brassica species (13 cultivars) were investigated. Seeds or F1 seedlings were easy to be produced from crosses × Brassicoraphanus × R. sativus, and B. napus, B. juncea and B. carinata × Brassicoraphanus. Fewer seeds or seedlings were obtained from crosses × Brassicoraphanus × B. napus, B. juncea and B. carinata. However, few seeds were harvested in the reciprocals of × Brassicoraphanus with B. rapa and B. oleracea. The possible cause of fertility improvements and the potential of the present × Brassicoraphanus for breeding were discussed.

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Acknowledgements

This work was supported by Huibei provincial project (2004AA204A-2), Chinese Ministry of Science and Technology (863 project, 2001AA241101), Changjiang Scholars and Innovative Research Team in University (IRT0442) PR China.

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Authors and Affiliations

  1. Faculty of Urban Construction and Environmental Engineering, Wuhan University of Science and Engineering, Wuhan, 430071, China

    H. G. Chen

  2. National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, 430070, China

    H. G. Chen & J. S. Wu

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  1. H. G. Chen
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  2. J. S. Wu
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Correspondence to J. S. Wu.

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Chen, H.G., Wu, J.S. Characterization of fertile amphidiploid between Raphanus sativus and Brassica alboglabra and the crossability with Brassica species. Genet Resour Crop Evol 55, 143–150 (2008). https://doi.org/10.1007/s10722-007-9223-8

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  • DOI: https://doi.org/10.1007/s10722-007-9223-8

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