Genetic Resources and Crop Evolution

, Volume 55, Issue 1, pp 33–43 | Cite as

Comparative analysis of genetic diversity using molecular and morphometric markers in Andrographis paniculata (Burm. f.) Nees

  • Surrinder K. Lattoo
  • Rekha S. Dhar
  • Shabnam Khan
  • S. Bamotra
  • Maharaj K. Bhan
  • Autar K. Dhar
  • Kuldip K. Gupta
Research Article


Andrographis paniculata is a medicinal plant of immense therapeutic value. The present study was aimed to elucidate its genetic diversity based on morphochemical and RAPD markers from 53 accessions belonging to 5 eco-geographic regions. Analysis of variance and D 2 statistics revealed significant differences in all the metric traits and sufficient inter-cluster distances indicating considerable diversity among the accessions. The complementary approach of RAPD was used to evaluate the genetic dissimilarities among all the accessions using 6 highly polymorphic primers. The average proportion of polymorphic loci across primers was 96.28%. The molecular genetic diversity based on Shannon index per primer averaged 5.585 with values ranging from 3.08 to 8.70 indicating towards wide genetic base. RAPD based UPGMA and D 2 cluster analysis also revealed that various accessions available in different eco-geographic regions might have originated from native places of wild abundance. Similarity matrices were generated for molecular markers and morphometric data to determine the degree of congruence between the two. A highly significant but low correlation (r = 0.547, P < 0.001) was obtained thus implying the correspondence between the two. The species is hermaphroditic and a habitual inbreeder. The present study yielded a typical triangular congruence between its breeding system, morphometric traits and RAPD markers thus elucidating the usefulness of complementary approaches to make diversity analysis more explanatory and purposeful for optimum genetic amelioration and effective conservation of its genotypic variability.


Andrographis paniculata Andrographolide Genetic diversity Mahalanobis D2 statistics Medicinal herb Shannon index 



The authors thank Dr. G.N. Qazi, Director Regional Research Laboratory (CSIR) for useful discussions. One of us (S.B.) was in receipt of research fellowship from GBPIHED, Almora, India, whilst the work was being carried out.


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Copyright information

© Springer Science+Business Media, Inc. 2007

Authors and Affiliations

  • Surrinder K. Lattoo
    • 1
  • Rekha S. Dhar
    • 2
  • Shabnam Khan
    • 1
  • S. Bamotra
    • 1
  • Maharaj K. Bhan
    • 1
  • Autar K. Dhar
    • 1
  • Kuldip K. Gupta
    • 3
  1. 1.Department of Genetics and Plant BreedingRegional Research Laboratory (CSIR)Jammu TawiIndia
  2. 2.Department of BiotechnologyRegional Research Laboratory (CSIR)Jammu TawiIndia
  3. 3.Division of Natural Products ChemistryRegional Research Laboratory (CSIR)Jammu TawiIndia

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