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Genetic Resources and Crop Evolution

, Volume 54, Issue 6, pp 1235–1244 | Cite as

RAPD and ISSR fingerprinting in cultivated chickpea (Cicer arietinum L.) and its wild progenitor Cicer reticulatum Ladizinsky

  • L. S. Rao
  • P. Usha Rani
  • P. S. Deshmukh
  • P. A. Kumar
  • S. K. Panguluri
Original Paper

Abstract

Detection of genetic relationships between 19 chickpea cultivars and five accessions of its wild progenitor Cicer reticulatum Ladizinsky were investigated by using RAPD and ISSR markers. On an average, six bands per primer were observed in RAPD analysis and 11 bands per primer in ISSR analysis. In RAPD, the wild accessions shared 77.8% polymorphic bands with chickpea cultivars, whereas they shared 79.6% polymorphic bands in ISSR analysis. In RAPD analysis 51.7% and 50.5% polymorphic bands were observed among wild accessions and chickpea cultivars, respectively. Similarly, 65.63% and 56.25% polymorphic bands were found in ISSR analysis. The dendrogram developed by pooling the data of RAPD and ISSR analysis revealed that the wild accessions and the ICCV lines showed similar pattern with the dendrogram of RAPD analysis. The ISSR analysis clearly indicated that even with six polymorphic primers, reliable estimation of genetic diversity could be obtained, while nearly 30 primers are required for RAPD. Moreover, RAPD can cause genotyping errors due to competition in the amplification of all RAPD fragments. The markers generated by ISSR and RAPD assays can provide practical information for the management of genetic resources. For the selection of good parental material in breeding programs the genetic data produced through ISSR can be used to correlate with the relationship measures based on pedigree data and morphological traits to minimize the individual inaccuracies in chickpea.

Keywords

Chickpea Genetic diversity ISSR’s RAPD’s Cicer arietinum 

Abbreviations

ISSR

Inter Simple Sequence Repeats

RAPD

Random Amplified Polymorphic DNA

Notes

Acknowledgements

Authors are grateful to Dr. S S Yadav, Principal Scientist and Dr. Jitender Kumar, Senior Scientist, Pulse Research Laboratory, IARI, New Delhi for providing seed material.

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Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  • L. S. Rao
    • 1
  • P. Usha Rani
    • 2
  • P. S. Deshmukh
    • 1
  • P. A. Kumar
    • 2
  • S. K. Panguluri
    • 3
  1. 1.Division of Plant PhysiologyIARINew DelhiIndia
  2. 2.NRC on Plant BiotechnologyIARINew DelhiIndia
  3. 3.Department of Entomology, College of AgricultureUniversity of KentuckyLexingtonUSA

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