RAPD and ISSR fingerprinting in cultivated chickpea (Cicer arietinum L.) and its wild progenitor Cicer reticulatum Ladizinsky
- 342 Downloads
Detection of genetic relationships between 19 chickpea cultivars and five accessions of its wild progenitor Cicer reticulatum Ladizinsky were investigated by using RAPD and ISSR markers. On an average, six bands per primer were observed in RAPD analysis and 11 bands per primer in ISSR analysis. In RAPD, the wild accessions shared 77.8% polymorphic bands with chickpea cultivars, whereas they shared 79.6% polymorphic bands in ISSR analysis. In RAPD analysis 51.7% and 50.5% polymorphic bands were observed among wild accessions and chickpea cultivars, respectively. Similarly, 65.63% and 56.25% polymorphic bands were found in ISSR analysis. The dendrogram developed by pooling the data of RAPD and ISSR analysis revealed that the wild accessions and the ICCV lines showed similar pattern with the dendrogram of RAPD analysis. The ISSR analysis clearly indicated that even with six polymorphic primers, reliable estimation of genetic diversity could be obtained, while nearly 30 primers are required for RAPD. Moreover, RAPD can cause genotyping errors due to competition in the amplification of all RAPD fragments. The markers generated by ISSR and RAPD assays can provide practical information for the management of genetic resources. For the selection of good parental material in breeding programs the genetic data produced through ISSR can be used to correlate with the relationship measures based on pedigree data and morphological traits to minimize the individual inaccuracies in chickpea.
KeywordsChickpea Genetic diversity ISSR’s RAPD’s Cicer arietinum
Inter Simple Sequence Repeats
Random Amplified Polymorphic DNA
Authors are grateful to Dr. S S Yadav, Principal Scientist and Dr. Jitender Kumar, Senior Scientist, Pulse Research Laboratory, IARI, New Delhi for providing seed material.
- Bornet B, Branchard M (2001) Non anchored inter simple sequence repeats (ISSR) markers reproducible and specific tools for genome finger printing. Plant Mol Biol Rep 19:209–215Google Scholar
- Doyle JJ, Doyle JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 19:11–15Google Scholar
- FAO (2004) http://faostat.fao.orgGoogle Scholar
- Gowda CLL (1981) Natural outcrossing in chickpea. Int Chickpea Newsl 5:6Google Scholar
- Malhotra RS, Pundir RPS, Slinkard AE (1987) Genetic resources of chickpea. In: Saxena MC, Singh KB (eds) The chickpea. CAB International, Wallingford, UK, pp 67–81Google Scholar
- Moussa EH, Millan T, Gil J, Cubero JI (1996) Variability and genome length estimation in chickpea (Cicer arietinum L.) revealed by RAPD analysis. J Genet Breed 51:83–85Google Scholar
- Simon CJ, Muehlbauer FJ (1997) Construction of chickpea linkage map and its comparison with the maps of pea and lentil. J Heridity 88:115–119Google Scholar
- Winter P, Benko-Iseppon AM, Huttel B, Ratnaparkhe MB, Tullu A, Sonnante G, Pfaff T, Tekeoglu M, Santra D, Sant VJ, Rajesh PN, Kahl G, Muehlbauer FJ (2000) A linkage map of the chickpea (Cicer arietinum L.) genome based on recombinant inbred lines from a C. arietinum × C. reticulatum cross: localization of resistance genes for fusarium races 4 and 5. Theor Appl Genet 101:1155–1163CrossRefGoogle Scholar