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Glycoconjugate Journal

, Volume 35, Issue 2, pp 161–163 | Cite as

On the use of cholera toxin

  • Elena Chiricozzi
  • Laura Mauri
  • Maria Grazia Ciampa
  • Alessandro Prinetti
  • Sandro Sonnino
Short Communication

Studies in the early 1970s revealed that the first step in cholera infection is represented by the interaction between the Vibrio cholerae exotoxin (Cholera Toxin, CT,) and the mucosal surface [1]. CT consists of a single catalytically active component A and a nontoxic pentamer of identical B subunits (B5).

Using in vitro approaches, the surface receptor was identified as the oligosaccharide β-Gal-(1–3)-β-GalNAc-(1–4)-[α-Neu5Ac-(2–3)-]β-Gal-(1–4)-β-Glc-, i.e., the ganglioside GM1 oligosaccharide [2]. Following studies established an association constant with CT in the range of 10−7 - 10−12 M, depending on the procedure used, and that the subunit A or the ganglioside ceramide moiety are not necessary for the association.

Then, CT derivatives and anti-CT antibodies for analytical immunostaining were rapidly developed for analytical purposes. Today immune-absorbent-, ELISA- and TLC- procedures, are available for detection and quantitation of the ganglioside GM1 through CT binding. In...

Keywords

Cholera toxin GM1 Glycolipids Glycoconjugates Immunostaining 

Notes

Compliance with ethical standards

Conflicts of interest

The authors declare that they have no conflicts of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

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© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Medical Biotechnology and Translational MedicineUniversity of MilanMilanItaly

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