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Glycoconjugate Journal

, Volume 33, Issue 5, pp 797–807 | Cite as

Chemical characterization of milk oligosaccharides of the tiger quoll (Dasyurus maculatus), a marsupial

  • Tadasu Urashima
  • Tomoko Yamamoto
  • Kentaro Hirayama
  • Kenji Fukuda
  • Tadashi Nakamura
  • Tadao Saito
  • Keith Newgrain
  • Jim Merchant
  • Brian Green
  • Michael Messer
Original Article

Abstract

Milk oligosaccharides were separated from the carbohydrate fraction of milk of the tiger quoll a species of marsupial that is closely related to the eastern quoll, Dasyurus viverrinus. They were characterized by 1H – nuclear magnetic resonance spectroscopy and matrix – assisted laser desorption/ionization time-of-flight mass spectrometry. The following oligosaccharides were identified; Gal(β1–3)Gal(β1–4)Glc, Gal(β1–3)Gal(β1–3)Gal(β1–4)Glc, Gal(β1–3)[Gal(β1–4)GlcNAc(β1–6)]Gal(β1–4)Glc, Gal(β1–3)Gal(β1–3)[Gal(β1–4)GlcNAc(β1–6)]Gal(β1–4)Glc, Gal(β1–3)[Gal(β1–4)GlcNAc(β1–6)]Gal(β1–3)Gal(β1–4)Glc, Gal(β1–3)[Gal(β1–3)Gal(β1–4)GlcNAc(β1–6)]Gal(β1–4)Glc, Gal(β1–3)[Gal(β1–4)GlcNAc(β1–6)]Gal(β1–3)[Gal(β1–4)GlcNAc(β1–6)]Gal(β1–4)Glc, Neu5Ac(α2–3) Gal(β1–3)[Gal(β1–4)GlcNAc(β1–6)]Gal(β1–3)[Gal(β1–4)GlcNAc(β1–6)]Gal(β1–4)Glc, Gal(β1–3)[Gal(β1–4)GlcNAc(β1–6)]Gal(β1–3)[Gal(β1–4)GlcNAc(β1–6)]Gal(β1–4)Glc with an α(2–3)Neu5Ac linked to β(1–4)Gal residue of either branch of Gal(β1–4)GlcNAc(β1–6) units, and Gal(β1–3)[Gal(β1–4)GlcNAc(β1–6)]Gal(β1–3)[Gal(β1–4)GlcNAc(β1–6)]Gal(β1–4)Glc with a β(1–3) linked Gal and an α(2–3) linked Neu5Ac. In addition, larger oligosaccharides were characterized as follows; Gal(β1–3){Gal(β1–3)[Gal(β1–4)GlcNAc(β1–6)]Gal(β1–4)GlcNAc(β1–6)}Gal(β1–3)[Gal(β1–4)GlcNAc(β1–6)]Gal(β1–4)Glc and Gal(β1–3)[Gal(β1–4)GlcNAc(β1–6)]Gal(β1–3){Gal(β1–3)[Gal(β1–4)GlcNAc(β1–6)]Gal(β1–4)GlcNAc(β1–6)}Gal(β1–4)Glc and their α(2–3) linked Neu5Ac derivatives.

Keywords

Tiger quoll Milk oligosaccharides Marsupials 

Supplementary material

10719_2016_9675_MOESM1_ESM.pdf (65 kb)
ESM 1 (PDF 64 kb)
10719_2016_9675_MOESM2_ESM.pdf (79 kb)
ESM 2 (PDF 79 kb)
10719_2016_9675_MOESM3_ESM.pdf (112 kb)
ESM 3 (PDF 112 kb)

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Tadasu Urashima
    • 1
  • Tomoko Yamamoto
    • 1
  • Kentaro Hirayama
    • 1
  • Kenji Fukuda
    • 1
  • Tadashi Nakamura
    • 2
  • Tadao Saito
    • 3
  • Keith Newgrain
    • 4
  • Jim Merchant
    • 5
  • Brian Green
    • 6
  • Michael Messer
    • 7
  1. 1.Graduate School of Animal and Food HygieneObihiro University of Agriculture & Veterinary MedicineObihiroJapan
  2. 2.Department of Food ScienceObihiro University of Agriculture & Veterinary MedicineObihiroJapan
  3. 3.Graduate School of AgricultureTohoku UniversitySendaiJapan
  4. 4.MagillAustralia
  5. 5.CookAustralia
  6. 6.Institute for Applied EcologyUniversity of CanberraCanberraAustralia
  7. 7.School of Molecular BiosciencesThe University of SydneySydneyAustralia

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