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Glycoconjugate Journal

, Volume 32, Issue 3–4, pp 113–118 | Cite as

Molecular structure of glycogen in diabetic liver

  • Bin Deng
  • Mitchell A. Sullivan
  • Jialun Li
  • Xinle Tan
  • Chengjun Zhu
  • Benjamin L. Schulz
  • Robert G. Gilbert
Short Communication

Abstract

Liver glycogen (involved in maintaining blood-sugar levels) is a hyperbranched glucose polymer containing β particles (diameter ~20 nm), which can form composite α particles (diameter ~50–300 nm), and includes a small but significant amount of bound protein. Size distributions of glycogen from livers of healthy and diabetic mice were examined using size-exclusion chromatography with two separate eluents: aqueous eluent and dimethylsulfoxide (DMSO) eluent. Morphologies were examined with transmission electron microscopy. Diabetic glycogen (DG) exhibited many α particles in the mild water-based solvent, but in DMSO, which breaks H bonds, these degraded to β particles; α particles however were always present in healthy glycogen (HG). This DG fragility shows the binding of β into α particles is different in HG and DG. The diabetic α particle fragility may be involved with the uncontrolled blood-sugar release symptomatic of diabetes: small β particles degrade more easily to glucose than α particles. This has implications for diabetes management.

Keywords

Glycogen db/db mice Size-exclusion chromatography Type 2 diabetes 

Notes

Acknowledgments

The support of the 1000-Talents program of the Chinese Foreign Experts Bureau, and an Australian Research Council Discovery grant, DP130102461, are gratefully acknowledged. We thank Dr. Enpeng Li and Mr. Shiqing Zhou for help with SEC analysis.

Supplementary material

10719_2015_9578_MOESM1_ESM.pdf (920 kb)
ESM 1 (PDF 920 kb)

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Bin Deng
    • 1
  • Mitchell A. Sullivan
    • 1
    • 2
  • Jialun Li
    • 3
  • Xinle Tan
    • 1
  • Chengjun Zhu
    • 2
  • Benjamin L. Schulz
    • 4
  • Robert G. Gilbert
    • 1
    • 2
  1. 1.School of Pharmacy, Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanChina
  2. 2.Centre for Nutrition and Food Science, Queensland Alliance for Agriculture and Food InnovationThe University of QueenslandBrisbaneAustralia
  3. 3.Department of Plastic Surgery, Wuhan Union Hospital, Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanChina
  4. 4.School of Chemistry and Molecular Biosciences, Faculty of ScienceThe University of QueenslandBrisbaneAustralia

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