Changes in bacterial glycolipids as an index of intestinal lactobacilli and epithelial glycolipids in the digestive tracts of mice after administration of penicillin and streptomycin
- 232 Downloads
The major lipid constituent of symbiotic gram-positive bacteria in animals are phosphatidylglycerol, cardiolipin and dihexaosyl diglycerides (DH-DG), whose hydrophobic structures are characteristic of the environments, and the carbohydrate structures of DH-DGs are bacterial species-characteristic. Immunization of rabbits with intestinal lactobacilli generated antibodies against DH-DGs and their modified structures, among which Galα1-6-substituted DH-DG, i.e., Lactobacillus tetrahexaosyl diglyceride (LacTetH-DG), reacted with antibodies more intensely than DH-DG. Whereas, from the 16S-rRNA sequence, the intestinal lactobacilli in murine digestive tracts were revealed to be L. johnsonii, in which LacTetH-DG is present at the concentration of 2.2 ng per 1 × 106 cells. To obtain more accurate estimates of intestinal lactobacilli in several regions of the digestive tract of mice, LacTetH-DG was detected by TLC-immunostaining with anti-Lactobacillus antisera, being found in the stomach, cecum and colon of normal breeding mice, 1.0 × 109, 3.5 × 109 and 7.4 × 109 cells, respectively. Administration of penicillin and streptomycin for 6 days resulted in a reduction in the number of intestinal lactobacilli, the levels being 0 %, 30 % and 4 % of the control ones in the stomach, cecum and colon, respectively, which was associated with the accumulation of the contents in the tracts from the stomach to the cecum and with diarrhea. In addition, a reduced amount of fucosyl GA1 (FGA1) and a compensatory increase in GA1 due to the reduced activity of α1,2-fucosyltransferase in the small intestine and the enhanced discharge of FGA1 into the contents occurred in mice, probably due to the altered population of bacteria caused by administration of penicillin and streptomycin.
KeywordsBacterial glycolipids TLC-immunostaining Asialo GM1 Fucosyl asialo GM1 Antibiotic-associated diarrhea Digestive contents
Penicillin and streptomycin
Lactobacillus dihexaosyl diglycerides
Lactobacillus trihexaosyl diglycerides
Lactobacillus tetrahexaosyl diglycerides.
This work was supported by JSPS KAKENHI Grant Numbers 24570141, 23592465 and 25462610.
- 1.Iwamori, M., Nakasa, M., Yamazaki, K., Iwamori, Y., Tanaka, K., Aoki, D., Adachi, S., Nomura, T.: Bacterial species-characteristic profiles of molecular species, and the antigenicity of phospholipids and glycolipids in symbiotic Lactobacillus, staphylococcus and streptococcus species. Glycoconj. J. 29, 199–209 (2012)PubMedCrossRefGoogle Scholar
- 2.Iwamori, M., Sakai, A., Minamimoto, N., Iwamori, Y., Tanaka, K., Aoki, D., Adachi, S., Nomura, T.: Characterization of novel glycolipid antigens with an α-galactose epitope in Lactobacilli detected with rabbit anti-Lactobacillus antisera and occurrence of antibodies against them in human sera. J. Biochem. 150, 515–523 (2011)PubMedCrossRefGoogle Scholar
- 3.Iwamori, M., Shibagaki, T., Nakata, Y., Adachi, S., Nomura, T.: Distribution of receptor glycolipids for Lactobacilli in murine digestive tract and production of antibodies cross-reactive with them by immunization of rabbits with Lactobacilli. J. Biochem. 146, 185–191 (2009)PubMedCrossRefGoogle Scholar
- 4.Iwamori, M., Iwamori, Y., Adachi, S., Nomura, T.: Excretion into feces of asialo GM1 in the murine digestive tract and Lactobacillus johnsonii exhibiting binding ability toward asialo GM1. A possible role of epithelial glycolipids in the discharge of intestinal bacteria. Glycoconj. J. 28, 21–30 (2011)PubMedCrossRefGoogle Scholar
- 5.Lin, B., Hayashi, Y., Saito, M., Sakakibara, Y., Yanagisawa, M., Iwamori, M.: GDP-fucose: beta-galactoside alpha1,2-fucosyltransferase, MFUT-II, and not MFUT-I or -III, is induced in a restricted region of the digestive tract of germ-free mice by host-microbe interactions and cycloheximide. Biochim. Biophys. Acta 1487, 275–285 (2000)PubMedCrossRefGoogle Scholar
- 7.Manual of microbiological culture media, Difco & BBL Manual, Becton, Dickinson and Company, Sparks, MD, USA, 290–293 (2003)Google Scholar
- 13.Lin, B., Saito, M., Sakakibara, Y., Hayashi, Y., Yanagisawa, M., Iwamori, M.: Characterization of three members of murine α1,2-fucosyltransferases: change in the expression of the Se gene in the intestine of mice after administration of microbes. Arch. Biochem. Biophys. 388, 207–215 (2001)PubMedCrossRefGoogle Scholar
- 14.Tanaka, S., Kobayashi, T., Songjinda, P., Tateyama, A., Tsubouchi, M., Kiyohara, C., Shirakawa, T., Sonomoto, K., Nakayama, J.: Influence of antibiotic exposure in the early postnatal period on the development of intestinal microbiota. FEMS Immunol. Med. Microbiol. 56, 80–87 (2009)PubMedCrossRefGoogle Scholar