Glycoconjugate Journal

, Volume 29, Issue 5–6, pp 379–387 | Cite as

Structural characterization of a homogalacturonan from Capparis spinosa L. fruits and anti-complement activity of its sulfated derivative

  • Huijun Wang
  • Hongwei Wang
  • Songshan Shi
  • Jinyou Duan
  • Shunchun Wang


A water-soluble polysaccharide CSPS-2B-2 with a molecular mass of 8.8 kDa, was obtained from the fruits of Capparis spinosa L. Chemical and NMR spectral analysis verified CSPS-2B-2 was a linear poly-(1-4)-α-D-galactopyranosyluronic acid in which 12.9 ± 0.4 % of carboxyl groups existed as methyl ester and 2.6 ± 0.1 % of D-GalpA residues were acetylated. A sulfated derivative Sul-2B-2 with a sulfation degree of 0.88 ± 0.02 was prepared via the substitution of C-2 and/or C-3 of GalpA residues in CSPS-2B-2. Bioassay on the complement and coagulation system demonstrated that Sul-2B-2 (CH50: 3.5 ± 0.2 μg/mL) had a stronger inhibitory effect on the activation of complement system through the classic pathway than that of heparin (CH50: 8.9 ± 0.3 μg/mL). Interestingly, Sul-2B-2 at low dose even middle dose (for example 52 μg/mL) had no effect on coagulation system, which was totally different from heparin. Thus, our observation indicated that Sul-2B-2 was more efficient than heparin in inhibiting the activation of the complement system through classical pathway and exhibiting a relatively less anti-coagulant activity. These results suggested that the sulfated derivative Sul-2B-2 prepared from the homogalacturonan in the fruits of Capparis spinosa L, might be a promising drug candidate in case of necessary therapeutic complement inhibition.


Anti-complement Homogalacturonan Sulfated derivative Inhibition hemolysis Anti-coagulant 





high performance gel permeation chromatography


nuclear magnetic resonance spectroscopy


classical pathway


alternative pathway


mannan binding lectin pathway


acute respiratory distress syndrome


trifluoroacetic acid


thin-layer chromatography


gel-permeation chromatography


gas chromatograph-mass spectrometer


gas chromatograph-flame ionization detector


1-cyclohexyl-3-(2-morpholinoethyl) carbodiimidemetho-p-toluenesulphonate


dimethyl sulfoxide


infrared absorption spectroscopy


normal human serum


platelet poor plasma


barbital buffered saline


recalcification time


thrombin time


degree of substitution


50 % complement hemolysis



This research was funded by New Drug Creaction and Manufacturing Program (2012ZX09301001-003 to SCW) and Program for New Century Excellent Talents in University (NCET-10-0886 to SCW). The authors are very grateful to Mr. Jian Yao and Dr. Kan Ding at Shanghai Institute of Materia Medica, Chinese Academy of Sciences for the NMR analysis.


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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Huijun Wang
    • 2
  • Hongwei Wang
    • 2
  • Songshan Shi
    • 2
  • Jinyou Duan
    • 1
  • Shunchun Wang
    • 2
    • 3
  1. 1.College of ScienceNorthwest A&F UniversityYanglingChina
  2. 2.Institute of Chinese Materia MedicaShanghai University of Traditional Chinese MedicineShanghaiChina
  3. 3.Shanghai University of Traditional Chinese MedicineShanghaiChina

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