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Glycoconjugate Journal

, Volume 29, Issue 4, pp 199–209 | Cite as

Bacterial species-characteristic profiles of molecular species, and the antigenicity of phospholipids and glycolipids in symbiotic Lactobacillus, Staphylococcus and Streptococcus species

  • Masao Iwamori
  • Masanori Nakasa
  • Kentaro Yamazaki
  • Yuriko Iwamori
  • Kyoko Tanaka
  • Daisuke Aoki
  • Shigeki Adachi
  • Taisei Nomura
Article

Abstract

Human symbiotic bacteria, Lactobacillus reuteri (LR) in the intestines, Staphylococcus epidermidis (SE) in skin and Streptococcus salivalis (SS) in the oral cavity, contain dihexaosyl diglycerides (DH-DG) in concentrations equivalent to those of phosphatidyl glycerol (PG) and cardiolipin (CL), together with mono- to tetrahexaosyl DGs. The molecular species, as the combination of fatty acids in the DG moiety, were revealed to be bacterial species-characteristic, but to be similar between glycolipids and phospholipids in individual bacteria, the major ones being 16:0 and cy19:0 for LR, ai15:0 and ai17:0 for SE, and 16:0 and 18:1 for SS, respectively. The carbohydrate structures of DH-DGs were also bacterial species-characteristic, being Galα1-2Glcα for LR, Glcβ1-6Glcβ for SE, and Glcα1-2Glcα for SS, respectively. Also, bacterial glycolipids were revealed to provide antigenic determinants characteristic of bacterial species on immunization of rabbits with the respective bacteria. Anti-L. johnsonii antiserum intensely reacted with tri- and tetrahexaosyl DGs, in which Galα was bound to DH-DG through an α1-6 linkage, as well as with DH-DG from LR. Although anti-SE antiserum preferentially reacted with DH-DG from SE, anti-SS antiserum reacted with DH-DG from SS and, to a lesser extent, with DH-DGs from LR and SE. But, both anti-SE and anti-SS antiserum did not react at all with monohexaosyl DG or glycosphingolipids with the same carbohydrates at the nonreducing terminals. In addition, 75 % of human sera, irrespective of the ABO blood group, were found to contain IgM to tri- and tetrahexaosyl DGs from LR, but not to DH-DGs from LR, SE and SS.

Keywords

Symbiotic bacteria Glycerophospholipids Glyceroglycolipids Sphingoglycolipids Anti-bacterial antiserum TLC-immunostaining 

Abbreviations: The nomenclature for glycosphingolipids is based on the recommendations of the IUPAC-IUB Commission on Biochemical Nomenclature [1].

CL

cardiolipin

DG

diglycerides

MH-DG

monohexaosyl diglycerides

DH-DG

dihexaosyl diglycerides

PA

phosphatidic acid

PG

phosphatidyl glycerol

TH-DG

trihexaosyl diglycerides

TetH-DG

tetrahexaosyl diglycerides

cy

cyclopropane

ai

anteiso

LJ

Lactobacillus johnsonii

LR

Lactobacillus reuteri

SE

Staphylococcus epidermidis

SS

Streptococcus salivalis

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Masao Iwamori
    • 1
  • Masanori Nakasa
    • 1
  • Kentaro Yamazaki
    • 1
  • Yuriko Iwamori
    • 1
  • Kyoko Tanaka
    • 2
  • Daisuke Aoki
    • 2
  • Shigeki Adachi
    • 3
  • Taisei Nomura
    • 3
  1. 1.Department of Biochemistry, Faculty of Science and TechnologyKinki UniversityHigashiosakaJapan
  2. 2.Department of Obstetrics and Gynecology, School of MedicineKeio UniversityTokyoJapan
  3. 3.Animal Models of Human DiseasesNational Institute of Biomedical InnovationIbarakiJapan

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