Glycoconjugate Journal

, Volume 29, Issue 1, pp 47–55 | Cite as

Asn54-linked glycan is critical for functional folding of intercellular adhesion molecule-5



Intercellular adhesion molecule-5 (ICAM-5, telencephalin) is a dendritically polarized type I membrane glycoprotein, and promotes dendritic filopodia formation. Although we have determined the N-glycan structures of ICAM-5 in a previous report, their function is unknown. Here, we produced fifteen ICAM-5 gene constructs, in which each potential N-glycosylation site was mutated, to elucidate the function of the N-glycans of ICAM-5, and observed the effects of transfection of them on a neuronal cell line, Neuro-2a (N2a). Only the N54Q mutant, which is the mutant for the most N-terminal glycosylation site, failed to induce filopodia-like protrusions in N2a cells. Immunofluorescence staining and cell surface biotinylation revealed that N54Q ICAM-5 was confined to the ER and also could not be expressed on the cell surface. This is further supported by the biochemical evidence that almost all N-glycans of N54Q ICAM-5 were digested by Endo glycosidase H and peptide:N-glycanase, indicating that almost all of them retain high-mannose-type structures in ER. In additon, it also failed to form disulfide bonds or functional protein complexes. The stable transformants of N54Q ICAM-5 showed retarded cell growth, but it was interesting that there was no apparent ER stress, because the mutant was sequentially degraded via ER associated degradation pathway by comparing the susceptibilities of the responses to various inhibitors of this pathway in wild-type and N54Q ICAM-5 transfectants. Taken together, the Asn54-linked glycan is necessary for normal trafficking and function of ICAM-5, but is unassociated with ER-associated degradation of it.


ICAM-5 N-glycan Site-directed mutagenesis Protein trafficking 

Supplementary material

10719_2011_9363_MOESM1_ESM.pdf (47 kb)
Fig. S1 DNA contents of parental N2a cells and, stable transfectannts expressing empty vector (mock), WT ICAM-5, or the N54Q mutant. (PDF 46 kb)
10719_2011_9363_MOESM2_ESM.pdf (39 kb)
Fig. S2 Amino-terminal N-glycan-deficient ICAM-1 is expressed on cell surfaces. (PDF 39 kb)
10719_2011_9363_MOESM3_ESM.pdf (35 kb)
Supplementary data 3 Supplemental materials and methods. (PDF 34 kb)


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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.Department of Applied Biological Science, Faculty of Science and TechnologyTokyo University of ScienceNodaJapan
  2. 2.Department of BiochemistryNagoya University Graduate School of MedicineNagoyaJapan
  3. 3.Department of Cell Biology and NeuroscienceJuntendo University Graduate School of MedicineBunkyo-kuJapan
  4. 4.Department of Radiation Medicine, Research Institute for Radiation Biology and MedicineHiroshima UniversityMinami-kuJapan

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