Glycoconjugate Journal

, Volume 27, Issue 5, pp 525–531 | Cite as

SIGNR1 ligation on murine peritoneal macrophages induces IL-12 production through NFκB activation



We have previously shown that murine resident peritoneal macrophages (PEMs) are activated in response to uptake of oligomannose-coated liposomes (OMLs), leading to production of interleukin (IL)-12. To understand the mechanism of activation of PEMs by OMLs, in the present study we investigated the role of a mannose-binding C-type lectin receptor, SIGNR1, in production of proinflammatory cytokines by PEMs, in which SIGNR1 acts as a physiological receptor for OMLs. Engagement of SIGNR1 on PEMs with an anti-SIGNR1-specific rat IgM antibody, ERTR9, induced production of IL-12 and tumor necrosis factor (TNF)-α from PEMs, while secretion of IL-6 and IL-1β was not detected with the same treatment. The level of phosphorylated IκB kinase in PEMs also increased in response to ERTR9 treatment of the cells. Treatment of PEMs with a specific nuclear factor kappa-B (NFκB) inhibitor, BAY11-7082, reduced ERTR9-dependent IL-12 production. Intraperitoneal treatment with BAY11-7082 also led to reduction of subsequent OML-induced IL-12 production from PEMs. These results indicate that SIGNR1-mediated intercellular signaling may induce production of cytokines such as IL-12 through NFκB activation.


C-type lectin Interleukin-12 Macrophage Nuclear factor kappa-B SIGNR1 



antigen-presenting cell


dendritic cell-specific ICAM-3-grabbing nonintegrins


oligomannose-coated liposome


resident peritoneal macrophage


SIGN-related 1



This work was supported by the Program for Promotion of Basic Research Activities for Innovative Biosciences (PROBRAIN), and in part by a Grant-in-Aid for Special Research from The Promotion and Mutual Aid Corporation for Private Schools of Japan.


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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  1. 1.Institute of GlycoscienceTokai UniversityHiratsukaJapan
  2. 2.Hiratsuka-shiJapan

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