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Glycosyltransferase and sulfotransferase gene expression profiles in human monocytes, dendritic cells and macrophages

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Abstract

Using a focused glycan-gene microarray, we compared the glycosyltransferase (GT) and sulfotransferase gene expression profiles of human monocytes, dendritic cells (DCs) and macrophages (Mϕs), isolated or differentiated from the same donors. Microarray analysis indicated that monocytes express transcripts for a full set of enzymes involved in the biosynthesis of multi-multiantennary branched N-glycans, potentially elongated by poly-N-acetyl-lactosamine chains, and of mucin-type Core 1 and Core 2 sialylated O-glycans. Monocytes also express genes involved in the biosynthesis and modification of glycosaminoglycans, but display a limited expression of GTs implicated in glycolipid synthesis. Among genes expressed in monocytes (90 out of 175), one third is significantly modulated in DCs and Mϕ respectively, most of them being increased in both cell types relative to monocytes. These changes might potentially enforce the capacity of differentiated cells to synthesize branched N-glycans and mucin-type O-glycans and to remodel cell surface proteoglycans. Stimulation of DCs and Mϕs with lipopolysaccharide caused a general decrease in gene expression, mainly affecting genes found to be positively modulated during the differentiation steps. Interestingly, although a similar set of enzymes are modulated in the same direction in mature DCs and Mϕs, cell specific genes are also differentially regulated during maturation, a phenomenon that may sustain functional specificities. Validation of this analysis was provided by quantitative real-time PCR and flow cytometry of cell surface glycan antigens. Collectively, this study implies an important modification of the pattern of glycosylation in DCs and Mϕs undergoing differentiation and maturation with potential biological consequences.

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Abbreviations

APC:

Antigen presenting cell

DC:

Dendritic cell

Ag:

Antigen

GSL:

Glycosphingolipid

GT:

Glycosyltransferase

Mϕ:

Macrophage

TLR:

Toll-like receptor

sLex :

Sialyl Lex

GAG:

Glycosaminoglycan

ST:

Sialyltransferase

PSA:

Polysialic acid

HS:

Heparan sulfate

qPCR:

Quantitative real-time PCR

LPS:

Lipopolysaccharide

MFI:

Mean fluorescence intensity

GM-CSF:

Granulocyte Mϕ-colony stimulating factor

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Acknowledgments

We acknowledge Dr Juan J. Garcia-Vallejo (VU Medical Center, Amsterdam, The Netherlands) for the gift of some oligonucleotides used for the qPCR analysis. We also thank Pr. R. Gerardy-Schahn (Medizinische Hochschule, Hannover, Germany) for the gift of the anti-PSA Ab.

This work was supported by the Institut National de la Santé et de la Recherche Médicale, the Pasteur Institute of Lille, the University of Lille 2, the Contrat de Plan Etat Région 2000–2006 (CPER)/FEDER (Fonds Européen de Développement Régional) and l’Agence Nationale de la Recherche (ANR) (program MIE grant R08066ES/RPV08036ESA). This work was also supported by Consortium for Functional Glycomics Grant GM-62116 from the National Institutes of Health. CP was recipient of a doctoral fellowship from the Conseil Régional Nord—Pas-de-Calais / INSERM. FT is supported by the Centre National de la Recherche Scientifique, CF and PG by the INSERM and SGD, MAKR and PD by the University of Lille 1.

Conflict of interest statement

None declared.

Author information

Authors and Affiliations

  1. Inserm, U547, Lille, 59019, France

    François Trottein, Stoyan Ivanov, Christophe Paget, Catherine Vendeville & Christelle Faveeuw

  2. Institut Pasteur de Lille, Lille, 59019, France

    François Trottein, Stoyan Ivanov, Christophe Paget, Catherine Vendeville, Christelle Faveeuw & Philippe Gosset

  3. Université Lille Nord de France, 59000, Lille, France

    François Trottein, Stoyan Ivanov, Christophe Paget, Catherine Vendeville, Aurélie Cazet, Sophie Groux-Degroote, Marie-Ange Krzewinski-Recchi, Christelle Faveeuw, Philippe Gosset & Philippe Delannoy

  4. The SCRIPPS Research Institute, La Jolla, CA, USA

    Lana Schaffer, Suzanna Lee & Steven R. Head

  5. UMR 8576, CNRS, Villeneuve d’Ascq, 59655, France

    Aurélie Cazet, Sophie Groux-Degroote, Marie-Ange Krzewinski-Recchi & Philippe Delannoy

  6. INSERM, U774, Lille, 59019, France

    Philippe Gosset

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Correspondence to François Trottein or Philippe Delannoy.

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Trottein, F., Schaffer, L., Ivanov, S. et al. Glycosyltransferase and sulfotransferase gene expression profiles in human monocytes, dendritic cells and macrophages. Glycoconj J 26, 1259–1274 (2009). https://doi.org/10.1007/s10719-009-9244-y

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  • DOI: https://doi.org/10.1007/s10719-009-9244-y

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