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Glycoconjugate Journal

, 26:1259 | Cite as

Glycosyltransferase and sulfotransferase gene expression profiles in human monocytes, dendritic cells and macrophages

  • François Trottein
  • Lana Schaffer
  • Stoyan Ivanov
  • Christophe Paget
  • Catherine Vendeville
  • Aurélie Cazet
  • Sophie Groux-Degroote
  • Suzanna Lee
  • Marie-Ange Krzewinski-Recchi
  • Christelle Faveeuw
  • Steven R. Head
  • Philippe Gosset
  • Philippe Delannoy
Article

Abstract

Using a focused glycan-gene microarray, we compared the glycosyltransferase (GT) and sulfotransferase gene expression profiles of human monocytes, dendritic cells (DCs) and macrophages (Mϕs), isolated or differentiated from the same donors. Microarray analysis indicated that monocytes express transcripts for a full set of enzymes involved in the biosynthesis of multi-multiantennary branched N-glycans, potentially elongated by poly-N-acetyl-lactosamine chains, and of mucin-type Core 1 and Core 2 sialylated O-glycans. Monocytes also express genes involved in the biosynthesis and modification of glycosaminoglycans, but display a limited expression of GTs implicated in glycolipid synthesis. Among genes expressed in monocytes (90 out of 175), one third is significantly modulated in DCs and Mϕ respectively, most of them being increased in both cell types relative to monocytes. These changes might potentially enforce the capacity of differentiated cells to synthesize branched N-glycans and mucin-type O-glycans and to remodel cell surface proteoglycans. Stimulation of DCs and Mϕs with lipopolysaccharide caused a general decrease in gene expression, mainly affecting genes found to be positively modulated during the differentiation steps. Interestingly, although a similar set of enzymes are modulated in the same direction in mature DCs and Mϕs, cell specific genes are also differentially regulated during maturation, a phenomenon that may sustain functional specificities. Validation of this analysis was provided by quantitative real-time PCR and flow cytometry of cell surface glycan antigens. Collectively, this study implies an important modification of the pattern of glycosylation in DCs and Mϕs undergoing differentiation and maturation with potential biological consequences.

Keywords

Dendritic cells Glycosyltransferases Monocytes Macrophages Microarray 

Abbreviations

APC

Antigen presenting cell

DC

Dendritic cell

Ag

Antigen

GSL

Glycosphingolipid

GT

Glycosyltransferase

Macrophage

TLR

Toll-like receptor

sLex

Sialyl Lex

GAG

Glycosaminoglycan

ST

Sialyltransferase

PSA

Polysialic acid

HS

Heparan sulfate

qPCR

Quantitative real-time PCR

LPS

Lipopolysaccharide

MFI

Mean fluorescence intensity

GM-CSF

Granulocyte Mϕ-colony stimulating factor

Notes

Acknowledgments

We acknowledge Dr Juan J. Garcia-Vallejo (VU Medical Center, Amsterdam, The Netherlands) for the gift of some oligonucleotides used for the qPCR analysis. We also thank Pr. R. Gerardy-Schahn (Medizinische Hochschule, Hannover, Germany) for the gift of the anti-PSA Ab.

This work was supported by the Institut National de la Santé et de la Recherche Médicale, the Pasteur Institute of Lille, the University of Lille 2, the Contrat de Plan Etat Région 2000–2006 (CPER)/FEDER (Fonds Européen de Développement Régional) and l’Agence Nationale de la Recherche (ANR) (program MIE grant R08066ES/RPV08036ESA). This work was also supported by Consortium for Functional Glycomics Grant GM-62116 from the National Institutes of Health. CP was recipient of a doctoral fellowship from the Conseil Régional Nord—Pas-de-Calais / INSERM. FT is supported by the Centre National de la Recherche Scientifique, CF and PG by the INSERM and SGD, MAKR and PD by the University of Lille 1.

Conflict of interest statement

None declared.

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Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • François Trottein
    • 1
    • 2
    • 3
  • Lana Schaffer
    • 4
  • Stoyan Ivanov
    • 1
    • 2
    • 3
  • Christophe Paget
    • 1
    • 2
    • 3
  • Catherine Vendeville
    • 1
    • 2
    • 3
  • Aurélie Cazet
    • 3
    • 5
  • Sophie Groux-Degroote
    • 3
    • 5
  • Suzanna Lee
    • 4
  • Marie-Ange Krzewinski-Recchi
    • 3
    • 5
  • Christelle Faveeuw
    • 1
    • 2
    • 3
  • Steven R. Head
    • 4
  • Philippe Gosset
    • 2
    • 3
    • 6
  • Philippe Delannoy
    • 3
    • 5
  1. 1.Inserm, U547LilleFrance
  2. 2.Institut Pasteur de LilleLilleFrance
  3. 3.Université Lille Nord de FranceLilleFrance
  4. 4.The SCRIPPS Research InstituteLa JollaUSA
  5. 5.UMR 8576CNRSVilleneuve d’AscqFrance
  6. 6.INSERM, U774LilleFrance

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