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High levels of E4-PHA-reactive oligosaccharides: potential as marker for cells with characteristics of hepatic progenitor cells

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Abstract

Oligosaccharides serve as markers of the cell surface and have been used as certain kinds of tumor markers. In the present study, we established a simple method for isolating hepatic progenitor cells using a lectin, which recognizes a characteristic oligosaccharide structure. Rat liver epithelial (RLE) cells, which have been established as a hepatic stem-like cell, were used to identify characteristic oligosaccharide structures on hepatic stem cells. As a result from lectin micro array, several types of lectin including E4-PHA were identified to bind RLE cells specifically. Furthermore, lectin blot and lectin flow cytometry analyses showed that binding to E4-PHA lectin was significantly increased in RLE cells, compared to hepatocytes, and hepatoma cells. The induction of differentiation into a hepatocyte lineage of RLE cells by treatment with Oncostatin M and dexamethasone resulted in a decrease in E4-PHA binding. Using an E4-PHA column, we succeeded in isolating hepatic stem cells from LEC (Long-Evans with cinnamon coat color) rat livers with fluminant hepatitis. The characteristics of the established cells were similar to RLE cells and had a potential of proliferating in rat liver. These results suggest that oligosaccharides can serve as a novel marker for the isolation of the hepatic progenitor cells.

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Abbreviations

RLE cell:

rat liver epithelial cell

E4-PHA:

Erythroagglutinating phytohemagglutinin

LCA:

Lens culinaris agglutinin

WGA:

Wheat germ agglutinin

LEC rats:

Long-Evans with cinnamon coat color rats

GFP:

Green Fluorescence Protein

LEC-HP cells:

LEC rat-derived hepatic progenitor cells

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Acknowledgement

We would like to thank Dr. Snorri S Thorgeirsson (National Cancer Institute/National Institutes Health) for providing RLE cells. Japan Science and Technology Agency (JST) and the 21st Century Center of Excellence (COE) program and the Global COE program of Osaka University from the Ministry of Education, Culture, Sports, Science, and Technology of Japan supported this work, in part.

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Authors and Affiliations

  1. Department of Molecular Biochemistry & Clinical Investigation, Osaka University Graduate School of Medicine, 1-7 Yamada-oka, Suita, 565-0871, Japan

    Nozomi Sasaki, Kenta Moriwaki, Katsuhisa Noda & Eiji Miyoshi

  2. CREST, Japan Science and Technology Agency, 4-1-8 Honcho Kawaguchi, Saitama, 332-0012, Japan

    Naofumi Uozumi, Nobuto Koyama & Eiji Miyoshi

  3. Department of Disease Glycomics, Research Institute for Microbial Diseases, Osaka University, 2-1 Yamada-oka, Suita, 565-0871, Japan

    Naoyuki Taniguchi

  4. Glycogene Function Team, Research Center for Glycoscience, National Institute of Advanced Industrial Science and Technology, OSL C-2, 1-1-1 Umezono, Tsukuba, Ibaraki, 305-8568, Japan

    Akihiko Kameyama & Hisashi Narimatsu

  5. Glycomics Research Laboratory, Moritex Co., Yokohama, Kanagawa, 225-0012, Japan

    Shunsaku Takeishi & Masao Yamada

  6. Takara Bio Inc., 3–4–1, Seta, Otsu, Shiga, 520–2193, Japan

    Nobuto Koyama

Authors
  1. Nozomi Sasaki
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  11. Eiji Miyoshi
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Corresponding author

Correspondence to Eiji Miyoshi.

Additional information

Nozomi Sasaki and Kenta Moriwaki equally contributed to this study.

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Sasaki, N., Moriwaki, K., Uozumi, N. et al. High levels of E4-PHA-reactive oligosaccharides: potential as marker for cells with characteristics of hepatic progenitor cells. Glycoconj J 26, 1213–1223 (2009). https://doi.org/10.1007/s10719-009-9240-2

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  • DOI: https://doi.org/10.1007/s10719-009-9240-2

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